2000
DOI: 10.1590/s0103-90162000000300028
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Abstract: A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.

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Cited by 19 publications
(9 citation statements)
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“…Further characterization of the antagonistic isolate was performed using 16SrRNA gene sequence. In brief, the total genomic DNA was isolated from ETR17 by CTAB method [ 45 ]. Amplification of the 16S rRNA gene was performed in 25μl reaction using Universal primers following specific conditions on a thermal cycler (Applied Biosystems GeneAmp PCR 2400) [ 39 ].…”
Section: Methodsmentioning
confidence: 99%
“…Further characterization of the antagonistic isolate was performed using 16SrRNA gene sequence. In brief, the total genomic DNA was isolated from ETR17 by CTAB method [ 45 ]. Amplification of the 16S rRNA gene was performed in 25μl reaction using Universal primers following specific conditions on a thermal cycler (Applied Biosystems GeneAmp PCR 2400) [ 39 ].…”
Section: Methodsmentioning
confidence: 99%
“…Few studies have been conducted regarding PCR feasibility directly from vascular fluid over the years Comstock and Irey [4] Lopes et al [15] Wang et al [7]. Results from this work shows that fast methods of DNA extraction, which took approximately 2 hours for a total of 100 samples, may be as effective as more time-demanding and elaborated protocols that require around 24 hours to complete [16][17][18][19][20]. Therefore, large scale diagnosis of leaf scald in sugarcane may be performed through the DNA extraction techniques M2, M4 or M5, since these methods are fast, simple and do not use phenol as one of its reagents [21][22][23].…”
Section: Resultsmentioning
confidence: 99%
“…DNA was precipitated by adding equal volume of isopropanol, and washed with 70% ethanol and air dried. DNA pellets were suspended in 100 µL sterilized distilled water [ 22 ].…”
Section: Methodsmentioning
confidence: 99%