A simple method for determination of the factor VII-phospholipid complex using normotest

Dalaker, Knut; Janson, T.L.; Johnsen, Berit; Skartlien, Anne Helen; Prydz, Hans

doi:10.1016/0049-3848(87)90142-3

Cited by 11 publications

(12 citation statements)

References 9 publications

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“…Factor VII levels measured using the one-stage system ranged from 0.41 to 2.02 U/ml. The positive correlations be tween the clotting activities of factors VII (r = 0.825, p < 0.001) and VIIpl (r = 0.868, p < 0.0001), respectively, both measured in the two systems, were confirming earlier ob servations [14],…”

Section: Resultssupporting

confidence: 76%

“…Factor VII in plasma before and after incubation with phospholipase C was mea sured in parallel using a standard one-stage test system and the modified Normotest sys tem [14] in plasma samples collected from 10 normal subjects and from 27 patients representing all the different types of hyper lipoproteinemia. Factor VII levels measured using the one-stage system ranged from 0.41 to 2.02 U/ml.…”

Section: Resultsmentioning

confidence: 99%

“…Factors VII and VIIP| were measured using a Normotest system (Nycomed, Oslo, Norway) [13], as recently described [14], with some modifications. 9 vol of blood was collected into 1 vol of 0.129 mol/1 sodium citrate and centrifuged at 1,000 g for 30 min at 20 ° C. The supernatant was pipetted off and tested within 3 h after collection.…”

Section: Methodsmentioning

confidence: 99%

“…This phospholipid complex can be measured using Normotest [13], as recently reported by Dalaker et al [14],…”

Section: Introductionmentioning

confidence: 99%

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Increased Activity of Factor VII and Factor VII-Phospholipid Complex Measured Using a Normotest System in Subjects with Hyperlipidemia

Nordøy

Illingworth²,

Connor³

et al. 1990

Pathophysiol Haemos Thromb

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Coagulation factor VII and factor VII-phospholipid complex (factor VII_p1), an activated form of factor VII, were measured using a Normotest system in 128 patients with various types of hyperlipoproteinemias and in 45 control subjects. A positive correlation was found between both factors VII (p < 0.001) and VII_p1 (p < 0.0001) and serum triglycerides. Similar correlations were established with very low density lipoproteins. A positive correlation was also observed between factor VII_pι and total cholesterol, whereas inverse correlations were found with high-density lipoproteins for both factors VII and VII_p1• No positive correlation could be found between low-density lipoproteins and factors VII and VII_p1• This study has shown that patients with hyperlipoproteinemias associated with high serum triglyceride levels, with or without high cholesterol levels, have increased activity of factors VII and VII_p1, both previously associated with an increased risk of coronary heart disease.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…This phospholipid complex can be measured using Normotest [13], as recently reported by Dalaker et al [14],…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increased Activity of Factor VII and Factor VII-Phospholipid Complex Measured Using a Normotest System in Subjects with Hyperlipidemia

Nordøy

Illingworth²,

Connor³

et al. 1990

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

show abstract

“…It has been assumed that the plasma concentration of factor VII is lowered due to an impaired secretion level of factor VII in the R353Q variant (Hunault et al 1997). Because a significant positive correlation between plasma factor VII activity and Normotest values has been suggested, it is considered useful to adopt Normotest for the screening of coagulation factor VII activity abnormalities (Dalaker et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms of the Factor VII Gene Associated with the Low Activities of Vitamin K-Dependent Coagulation Factors in One-Month-Old Infants

Ito

Goto

Sugiura

et al. 2007

Tohoku J. Exp. Med.

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Despite administration of vitamin K (VK), some infants show lower activity of VK-dependent coagulation factors and they could develop intracranial hemorrhage. For preventing VK deficiency bleeding (VKDB) in infants, oral administration of VK and a screening test for VK deficiency are carried out in Japan. For the screening, the total activity of VK-dependent coagulation factors is measured using a commercial product, Normotest ® . This study was undertaken to clarify the importance of the following genetic and environmental factors on the coagulation status in one-month-old infants: two polymorphisms in the factor VII gene, -323P0/10 (a 10-bp insertion in the promoter region at position -323) and R353Q (the replacement of arginine [R] with glutamine [Q] at residue 353) and sex, age, gestational age, birth weight, and feeding regimen. Two hundred Japanese infants (34.6 ± 4.0 days old) were screened for VK-dependent coagulation activity with Normotest and were genotyped for the two polymorphisms. Among the subjects screened, 18 infants (9%) carried the P10 allele and 26 (13%) carried the R353Q allele. Multiple regression analysis showed that the 10-bp inserted (P10) allele or the Q allele was associated with the lower coagulation activities. The coagulation activities for the R/Q genotype were significantly lower than those for the R/R genotype and those for the P0/P10 genotype were significantly lower than those for the P0/P0 genotype. Therefore, infants who carry the P10 allele or the Q allele show lower activity of VK-dependent coagulation factors. These infants may have a higher risk of VKDB manifestation.

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n-3 Fatty Acids in Hypertension

Bønaa

1992

Fish Oil and Vascular Disease

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A simple method for determination of the factor VII-phospholipid complex using normotest

Cited by 11 publications

References 9 publications

Increased Activity of Factor VII and Factor VII-Phospholipid Complex Measured Using a Normotest System in Subjects with Hyperlipidemia

Increased Activity of Factor VII and Factor VII-Phospholipid Complex Measured Using a Normotest System in Subjects with Hyperlipidemia

Polymorphisms of the Factor VII Gene Associated with the Low Activities of Vitamin K-Dependent Coagulation Factors in One-Month-Old Infants

n-3 Fatty Acids in Hypertension

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