A simple LC-MS/MS method for the simultaneous quantification of endocannabinoids in biological samples

Bobrich, Maria; Schwarz, Rico; Ramer, Robert; Borchert, Philipp; Hinz, Burkhard

doi:10.1016/j.jchromb.2020.122371

Cited by 18 publications

(13 citation statements)

References 49 publications

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“…N -arachidonoyldopamine (NADA), O -arachidonoylethanolamide (VA), and 2-arachidonoylglycerol ether (2-AGE) derived from n-3 polyunsaturated fatty acids are also considered endocannabinoids. 17 Endocannabinoids, receptors, and enzymes (synthesize and degrade endocannabinoids) constitute the endocannabinoid system (ECS, Fig. 2 ).…”

Section: Chemical Structures Of Cannabinoidsmentioning

confidence: 99%

The impact of phyto- and endo-cannabinoids on central nervous system diseases：A review

Zhang

Zhang²,

Guo³

et al. 2023

Journal of Traditional and Complementary Medicine

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Section: Chemical Structures Of Cannabinoidsmentioning

confidence: 99%

The impact of phyto- and endo-cannabinoids on central nervous system diseases：A review

Zhang

Zhang²,

Guo³

et al. 2023

Journal of Traditional and Complementary Medicine

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“…Additionally, the duration of storage of serum prior to the measurement of endocannabinoids was variable, raising the potential for inconsistency due to endocannabinoid breakdown. To date the stability in canine serum has not been validated, however a human study by Bobrich et al found that AEA and 2-AG in serum were stable at 60 days when stored at −80 • C (46). Thirdly, the control group was small and was not intentionally age or weight matched to the lymphoma population.…”

Section: Discussionmentioning

confidence: 99%

Circulating Endocannabinoids in Canine Multicentric Lymphoma Patients

et al. 2022

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The endocannabinoid system is increasingly being implicated in the pathogenesis and progression of various human cancers. Specifically, increased levels of 2-arachidonoylglycerol (2-AG) and oleoythanolamide (OEA) have been demonstrated in human diffuse large B-cell lymphoma (DLBCL) and chronic lymphocytic leukemia (CLL) patients, respectively. The objectives of this paper were to compare 2-AG, OEA, N-arachidonoylethanolamine (AEA), and palmitoylethanolamide (PEA) levels between dogs with multicentric lymphoma and healthy control dogs. In addition, evaluate 2-AG, OEA, AEA, and PEA levels as biomarkers for progression free interval (PFI) and overall survival time (OST) in the dogs with lymphoma. The study consisted of 26 dogs with multicentric B cell lymphoma, 14 dogs with multicentric T cell lymphoma, and 12 healthy control dogs. Serum 2-AG, OEA, AEA, and PEA levels were measured using liquid chromatography combined with tandem mass spectrometry (LC—MS/MS) in dogs with lymphoma and in healthy dogs. OEA, AEA, and PEA levels were significantly elevated in dogs with lymphoma compared to healthy controls (p < 0.05). Total AG was significantly higher in healthy control dogs (p = 0.049). There was no significant difference between dogs with B cell and T cell lymphoma for any of the measured endocannabinoids. Elevated PEA was significantly associated with decreased PFI (p = 0.04) in dogs with lymphoma with a hazards ratio of 1.816 [95% Confidence Interval (CI): 1.020–3.232]. Overall, dogs with lymphoma have elevated levels of OEA, AEA, and PEA. PEA levels have the potential to be a prognostic biomarker.

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“…Sleep deprivation stimulates the EC system, especially 2-AG. 15 Besides the availability of competitive enzyme linked immunosorbent (ELISA) assay from various companies, to date, most of the researchers have used high performance liquid chromatography (HPLC), 16–18 ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) (UHPLC-MS/MS), 19,20 LC-MS/MS, 21 liquid chromatography electrospray ionization-mass spectrometry (LC-ESI-MS) 22,23 for identifying and monitoring 2-AG (Table 1). 28–32 Although the conventional methods have some advantages such as considerable sensitivity and specificity in the identification of 2-AG but they suffer from some disadvantages, for example time-consuming procedures, necessity for advanced equipment and experts.…”

Section: Introductionmentioning

confidence: 99%

An innovative electrochemical immuno-platform towards ultra-sensitive monitoring of 2-arachidonoyl glycerol in samples from rats with sleep deprivation: bioanalysis of endogenous cannabinoids using biosensor technology

et al. 2022

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A simple LC-MS/MS method for the simultaneous quantification of endocannabinoids in biological samples

Cited by 18 publications

References 49 publications

The impact of phyto- and endo-cannabinoids on central nervous system diseases：A review

The impact of phyto- and endo-cannabinoids on central nervous system diseases：A review

Circulating Endocannabinoids in Canine Multicentric Lymphoma Patients

An innovative electrochemical immuno-platform towards ultra-sensitive monitoring of 2-arachidonoyl glycerol in samples from rats with sleep deprivation: bioanalysis of endogenous cannabinoids using biosensor technology

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