A simple and robust LC-ESI single quadrupole MS-based method to analyze polyphenols in plant extracts using deep eutectic solvents

Letsiou, Sophia; Trapali, Maria; Tebbi, Sara Oumenoune; Debbache-Benaida, Nadjet

doi:10.1016/j.mex.2023.102303

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…The presence of polyphenols in the extracts was determined using a LC-ESI single quadrupole MS (Shimadzu, Kyoto, Japan) according to a previous work of our group [26]. Briefly, the mobile phase of the system was 95% ddH 2 O, 5% MeOH, 0.2% acetic acid (solvent A) and 50% acetonitrile (ACN)/50% ddH 2 O (solvent B) in gradient mode.…”

Section: Determination Of Total Phenolics Phenolic Acids and Flavonoidsmentioning

confidence: 99%

Exploring the Antifungal Activity of Various Natural Extracts in a Sustainable Saccharomyces cerevisiae Model Using Cell Viability, Spot Assay, and Turbidometric Microbial Assays

Letsiou,

Pyrovolou,

Konteles

et al. 2024

Applied Sciences

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Saccharomyces cerevisiae is a sustainable yeast with many applications in the food industry. Here, we study the use of a Saccharomyces cerevisiae model composed of three different industrial strains (a wine, a beer and a baker’s strain) to assess the antifungal activity of three organic plant-based extracts (Hypericum perforatum 10% w/w, Pistacia lentiscus var. Chia 20% w/w and Rosmarinus officinalis 6% w/w). Three different methods were employed (agar disc diffusion, spot assay, and growth curve analysis). Only the Rosmarinus officinalis extract (6% w/w) exhibited inhibitory activity against all the tested yeast strains in the agar disc diffusion method. In the spot assay, all extracts and their carrier oils (sunflower oil and caprylic triglyceride) exerted similar mild antifungal activity. In the growth curve analysis, all extracts significantly lowered the growth rate of the yeasts, but this was not observed for the carrier oils. The results highlighted that it is important to consider more than one method for testing the antimicrobial activity of different compounds. The three yeast strains exhibited differences in their susceptibility to pharmaceutical antifungals, and the beer and baker’s yeasts were resistant to itraconazole. Moreover, polyphenols were detected in all natural extracts which may be linked to their antifungal activity. Our results suggest that we might consider multiple use of these natural extracts in the food industry as food additives or even preservatives to delay food spoilage.

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Section: Determination Of Total Phenolics Phenolic Acids and Flavonoidsmentioning

confidence: 99%

Exploring the Antifungal Activity of Various Natural Extracts in a Sustainable Saccharomyces cerevisiae Model Using Cell Viability, Spot Assay, and Turbidometric Microbial Assays

Letsiou,

Pyrovolou,

Konteles

et al. 2024

Applied Sciences

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“…The chemical determination of the plant extract was based on LC-ESI single quadrupole MS (Shimadzu, Kyoto, Japan) using a recently published method [38] to assess the profile of the antioxidant metabolites in Origanum dictamnus L. extract. Briefly, the LC was performed on the reversed-phase SyncronisTM C-18 column 150 × 4.6 mm; particle size: 5 µm (Thermo Fisher Scientific TM , Waltham, MA, USA).…”

Section: Lc-(esi)/ms Analysis For Plant Extractmentioning

confidence: 99%

“…The identification was based on the retention times as well as overlay curves. Phenolic compounds were identified by comparison with the retention time of the standards of phenolic compounds [38]. The results were expressed as the mean value ± SD of three measurements.…”

Section: Lc-(esi)/ms Analysis For Plant Extractmentioning

confidence: 99%

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

Letsiou,

Trapali,

Vougiouklaki

et al. 2023

Cosmetics

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Skin aging mainly occurs due to intrinsic and extrinsic factors. Extrinsic aging is a consequence of exposure to ultraviolet radiation. Meanwhile, natural products exhibit protective properties against skin aging as well as photoaging. In this context, the research on natural anti-aging agents is greatly advanced, and in recent years, numerous plant-based products have been investigated. The aim of this study was to assess the antioxidant profile of Origanum dictamnus L. extract as well as its antiaging effects on 2D cultures of fibroblasts and keratinocytes under UVA irradiation to unravel the potential role of Origanum dictamnus L. in cosmetology. In an attempt to explore the antioxidant profile of the extract, we employed well-established enzymatic assays (DPPH, FRAP, ABTS, and TPC) and a phytochemical screening by LC/MS. According to our findings, the Origanum dictamnus L. extract possesses high scavenging activity (DPPH, ABTS), high phenolic content (TPC), and high Fe(III)-reduction activity (FRAP). Moreover, the LC/MS analysis revealed that the extract was rich in flavonoids, holding a high content of curcumin, kampferol, silymarin, cyanidin-3-glucoside, deosmin, rutin, and quercetin. To gain insight into the bioactivity of Origanum dictamnus L. extract in cell aging, the expression of various genes that are implicated in the skin aging process in keratinocytes and fibroblasts was studied. The gene expression analysis revealed that the extract increases cell proliferation in the cells exposed to UVA irradiation and concomitantly modulates the expression of genes related to the aging process in keratinocytes (KLK7, OCLN, GBA1) and fibroblasts (SIRT2, FOXO3, COL3A1) under the same conditions.

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Exploring the Anti-Diabetic, Antioxidant and Anti-Microbial Properties of Clematis flammula L. Leaves and Pistacia lentiscus L. Fruits Using Choline Chloride-Based Deep Eutectic Solvent

Tebbi,

Trapali,

Letsiou

2023

Waste Biomass Valor

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A simple and robust LC-ESI single quadrupole MS-based method to analyze polyphenols in plant extracts using deep eutectic solvents

Cited by 6 publications

References 17 publications

Exploring the Antifungal Activity of Various Natural Extracts in a Sustainable Saccharomyces cerevisiae Model Using Cell Viability, Spot Assay, and Turbidometric Microbial Assays

Exploring the Antifungal Activity of Various Natural Extracts in a Sustainable Saccharomyces cerevisiae Model Using Cell Viability, Spot Assay, and Turbidometric Microbial Assays

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

Exploring the Anti-Diabetic, Antioxidant and Anti-Microbial Properties of Clematis flammula L. Leaves and Pistacia lentiscus L. Fruits Using Choline Chloride-Based Deep Eutectic Solvent

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