A simple and rapid preparation of alditol acetates for monosaccharide analysis

Blakeney, A. B.; Harris, Philip J.; Henry, Robert J.; Stone, Bruce

doi:10.1016/0008-6215(83)88244-5

Cited by 1,879 publications

(834 citation statements)

References 22 publications

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“…Total uronic acids were measured spectrophotometrically, using authentic galacturonic acid as a standard (Blumenkrantz and Asboe-Hansen, 1973). Cell wall monosaccharide content was determined by gas chromatography essentially as described (Blakeney et al, 1983;Reiter et al, 1997), except that separations were performed on an Agilent 6890N gas chromatograph (Wilmington, DE).…”

Section: Cell Wall Analysismentioning

confidence: 99%

The PEN1 Syntaxin Defines a Novel Cellular Compartment upon Fungal Attack and Is Required for the Timely Assembly of Papillae

Assaad

Qiu

Youngs

et al. 2004

MBoC

344

382

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Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the plant secretes cell wall appositions or papillae beneath the penetration peg of the fungus. Genetic screens for mutations that result in increased penetration of Bgh on Arabidopsis have recently identified the PEN1 syntaxin. Here we examine the role of PEN1 and of its closest homologue, SYP122, identified as a syntaxin whose expression is responsive to infection. pen1 syp122 double mutants are both dwarfed and necrotic, suggesting that the two syntaxins have overlapping functions. Although syp122-1 and the cell wall mur mutants have considerably more pronounced primary cell wall defects than pen1 mutants, these have relatively subtle or no effects on penetration resistance. Upon fungal attack, PEN1 appears to be actively recruited to papillae, and there is a 2-h delay in papillae formation in the pen1-1 mutant. We conclude that SYP122 may have a general function in secretion, including a role in cell wall deposition. By contrast, PEN1 appears to have a basal function in secretion and a specialized defense-related function, being required for the polarized secretion events that give rise to papilla formation.

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Section: Cell Wall Analysismentioning

confidence: 99%

The PEN1 Syntaxin Defines a Novel Cellular Compartment upon Fungal Attack and Is Required for the Timely Assembly of Papillae

Assaad

Qiu

Youngs

et al. 2004

MBoC

344

382

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“…The neutral sugars rhamnose (Rha), fucose (Fuc), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal) and glucose (Glc), excluding the glucose derived from the cellulose, released by acid hydrolysis of the polysaccharides [2], were quantitatively determined by gas-liquid chromatography after reduction and acetylation [5,10,17]. About 1mg of freeze dried product was hydrolyzed in a screw-cap vial with 1mL of 2M trifluoroacetic acid (TFA) containing 1µmol of myo-inositol (internal standard)/90min/120°C in a heating block and submitted to stirring at 30min intervals towards the end of incubation, then cooled and centrifuged at 800g/5min.…”

Section: -Neutral Sugars Determinationmentioning

confidence: 99%

Cell wall polysaccharides of common beans (Phaseolus vulgaris L.)

Shiga¹,

Lajolo²,

Filisetti³

2003

Ciênc. Tecnol. Aliment.

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“…Component sugars were determined by the method proposed by Blakeney et al (1983). 10 mg (fresh weight basis) , and left at room temperature for 45 minutes.…”

Section: Content Analysis Of Monosacchridesmentioning

confidence: 99%

Component Analysis of Acorns of Quercus mongolica and Quercus Variabilis

Lee¹,

An²,

Han³

et al. 2016

Journal of Forest and Environmental Science

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To compare seed components of plus trees, seed powder ground after seedcoat removal was analyzed for two oak species, i. e., Quercus monglica (white oak) and Quercus variabilis (red oak), which are typical oak trees in Korea but have different fruiting characteristics. Thus we aimed at analyzing and comparing many ingredients including minerals, sugars, etc. Two species were similar to each other in the content of water, crude ash, crude protein and carbohydrates, but crude lipid content in Q. variabilis was 2.5 times higher than that in Q. mongolica. Crude proteins of Clone 124 was 1.5 times higher than that of Clone 75 in Q. mongolica. Crude lipid content showed the highest value in Clone 0511 of Q. variabilis, and more phosphate and iron was found in Q. monglica than in Q. variabilis. Glucose showed 85.4% and 88.3% on average of the total monosacchrides in two species, and galactose and arabinose were also found. In the content of phosphate, iron, and crude lipid, differences were found between two species and among clones of two species.

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A simple and rapid preparation of alditol acetates for monosaccharide analysis

Cited by 1,879 publications

References 22 publications

The PEN1 Syntaxin Defines a Novel Cellular Compartment upon Fungal Attack and Is Required for the Timely Assembly of Papillae

The PEN1 Syntaxin Defines a Novel Cellular Compartment upon Fungal Attack and Is Required for the Timely Assembly of Papillae

Cell wall polysaccharides of common beans (Phaseolus vulgaris L.)

Component Analysis of Acorns of Quercus mongolica and Quercus Variabilis

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