volume 16, issue 10, P912-917 1994
DOI: 10.1016/0141-0229(94)90067-1
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Khawar Sohail Siddiqui, Muhammad Hamid Rashid, Ahsan Mushir Shemsi, Muhammad Ibrahim Rajoka

Abstract: An apparatus based on electrophoresis has been devised that removes noncovalently bound polysaccharides from extracellular proteins of Aspergillus niger with concomitant partial beta-glucosidase purification and concentration. The apparatus consists of a series of three chambers separated by polyacrylamide gels. Dialyzed and concentrated crude extract of Aspergillus niger containing beta-glucosidase was poured into the middle chamber, while smaller anodic and cathodic chambers contained buffer. When electric c…

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