A signal conversion system using binding‐induced strand displacement for disease biomarker assay

Jiang, Yuling; Yang, Peng; Du, Lijie; Xia, Lingying; Chen, Junbo; Hou, Xiandeng

doi:10.1002/bio.4087

Cited by 7 publications

(6 citation statements)

References 25 publications

(28 reference statements)

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“…Using this strategy, protein dimerization, such as the dimerization of the Met receptor or the transforming growth factor-β type II receptor, was clearly visualized on the surface of living cells in real-time. To date, bivalent binding-induced DNA displacement has been used to sense streptavidin on magnetic beads [56,57], thrombin [58][59][60][61][62], PDGF [63][64][65], human epidermal growth factor receptor-2 (HER2) in live cells [66], human acute lymphoblastic leukemia cells (CCRF-CEM) [67], prostate-specific antigen (PSA) [68][69][70], antibodies [71][72][73][74], cardiac troponin [75], and other, specific cells [76]. Additionally, streptavidin has been detected using chemiluminescent detection based on a DNA walker [77], and antibodies have been identified through quantum dot doping methods [78].…”

Section: Biosensors Based On Dna Displacementmentioning

confidence: 99%

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

et al. 2022

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Several biological macromolecules adopt bivalent or multivalent interactions to perform various cellular processes. In this regard, the development of molecular constructs presenting multiple ligands in a specific manner is becoming crucial for the understanding of multivalent interactions and for the detection of target macromolecules. Nucleic acids are attractive molecules to achieve this goal because they are capable of forming various, structurally well-defined 2D or 3D nanostructures and can bear multiple ligands on their structures with precisely controlled ligand–ligand distances. Thanks to the features of nucleic acids, researchers have proposed a wide range of bivalent and multivalent binding agents that strongly bind to target biomolecules; consequently, these findings have uncovered new biosensing strategies for biomolecule detection. To date, various bivalent and multivalent interactions of nucleic acid architectures have been applied to the design of biosensors with enhanced sensitivity and target accuracy. In this review, we describe not only basic biosensor designs but also recently designed biosensors operating through the bivalent and multivalent recognition of nucleic acid scaffolds. Based on these designs, strategies to transduce bi- or multivalent interaction signals into readable signals are discussed in detail, and the future prospects and challenges of the field of multivalence-based biosensors are explored.

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Section: Biosensors Based On Dna Displacementmentioning

confidence: 99%

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

et al. 2022

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“…An AuNPs-tagged probe served as the reporter which tagged the AuNPs on the 3 0 -end, and the 5 0 -end was connected with magnetic beads (MBs) with the strong anti-interference ability of magnetic separation. 33 In the presence of the target, the ternary complex of target/crRNA/Cas12a formed and activated the trans-cleavage activity of CRISPR-Cas12a for collateral ssDNA. Then, the AuNPs-tagged probe was cleaved, and AuNPs were unleashed from the surface of the MBs.…”

mentioning

confidence: 99%

Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids

Zhou

Liu

et al. 2022

Chem. Commun.

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“…This activity usually requires metal ions as cofactors [120]. The other is that DNAzyme binds to hemin to form G-quadruplexes, mimics peroxidase activity, and connects to affinity ligands for signal transduction [121][122][123]. In recent years, modified DNAzyme has found widespread application as a mediator in FNA biosensors; for example, in environmental monitoring, food safety detection, and liquid analysis [42,115,124].…”

Section: Dnazyme Based Strategies In Enzyme Mediatorsmentioning

confidence: 99%

Recent Advances on Functional Nucleic-Acid Biosensors

Zhang

Guo

et al. 2021

Sensors

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In the past few decades, biosensors have been gradually developed for the rapid detection and monitoring of human diseases. Recently, functional nucleic-acid (FNA) biosensors have attracted the attention of scholars due to a series of advantages such as high stability and strong specificity, as well as the significant progress they have made in terms of biomedical applications. However, there are few reports that systematically and comprehensively summarize its working principles, classification and application. In this review, we primarily introduce functional modes of biosensors that combine functional nucleic acids with different signal output modes. In addition, the mechanisms of action of several media of the FNA biosensor are introduced. Finally, the practical application and existing problems of FNA sensors are discussed, and the future development directions and application prospects of functional nucleic acid sensors are prospected.

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A signal conversion system using binding‐induced strand displacement for disease biomarker assay

Cited by 7 publications

References 25 publications

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

Biosensors Based on Bivalent and Multivalent Recognition by Nucleic Acid Scaffolds

Dual-amplified CRISPR-Cas12a bioassay for HIV-related nucleic acids

Recent Advances on Functional Nucleic-Acid Biosensors

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