A sensitive transgenic plant system to detect toxic inorganic compounds in the environment

Kovalchuk, Olga; Титов, В. А.; Höhn, Barbara; Kovalchuk, Igor

doi:10.1038/89327

Cited by 104 publications

(51 citation statements)

References 30 publications

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“…Similarly, in contrast with Molinier et al (2005) and Yao et al (2011), who found a seven-or threefold increase in recombination frequency, respectively, as a result of UV-C irradiation in Arabidopsis, Van der Auwera et al (2008) did not find a significant effect of UV-C in their SHR lines containing GUS recombination reporter (Van der Auwera et al, 2008). Furthermore, in contrast with earlier results (Kovalchuk et al, 2001b;Boyko et al, 2005), Van der Auwera et al (2008) reported that the presence of heavy metals, such as lead or cadmium ions, a heat shock of 50˚C, or growth at elevated temperatures and increased daylength had no measurable effect on the mutation or recombination frequencies . In contrast with the reported 1.5-fold to sevenfold increased recombination level in two tested Arabidopsis lines as a reaction to spraying plants with the salicylic acid analogs INA and benzothiadiazole (Lucht et al, 2002), or the results of Yao et al (2011) who used methyl salicylate, Van der Auwera et al (2008) did not find a significant difference when they added sodium salicylate to the growth medium (Van der Auwera et al, 2008).…”

Section: Conflicting Results From Shr Reporter Linescontrasting

confidence: 55%

“…Analysis of several independent reporter lines carrying these marker genes indicated that the average somatic recombination frequency detected with the LUC transgene was ;10-fold higher (10 25 ) in Arabidopsis thaliana and tobacco (Nicotiana tabacum) plants compared with GUS recombination frequencies (Ilnytskyy et al, 2004). Using such GUS-or LUC-based SHR reporter lines, it was shown that abiotic and especially biotic stresses lead to enhanced somatic recombination (Kovalchuk et al, 1998(Kovalchuk et al, , 2001a(Kovalchuk et al, , 2001b(Kovalchuk et al, , 2003Lucht et al, 2002;Filkowski et al, 2004;Ilnytskyy et al, 2004;Molinier et al, 2004Molinier et al, , 2005Molinier et al, , 2006Schuermann et al, 2005). Using such a system, it was also discovered that plants can remember a stress perceived in one generation for several following generations.…”

Section: Somatic Homologous Recombination Reporter Lines: Lack Of Sufmentioning

confidence: 99%

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Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

et al. 2012

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A widely used approach for assessing genome instability in plants makes use of somatic homologous recombination (SHR) reporter lines. Here, we review the published characteristics and uses of SHR lines. We found a lack of detailed information on these lines and a lack of sufficient evidence that they report only homologous recombination. We postulate that instead of SHR, these lines might be reporting a number of alternative stress-induced stochastic events known to occur at transcriptional, posttranscriptional, and posttranslational levels. We conclude that the reliability and usefulness of the somatic homologous recombination reporter lines requires revision. Thus, more detailed information about these reporter lines is needed before they can be used with confidence to measure genome instability, including the complete sequences of SHR constructs, the genomic location of reporter genes and, importantly, molecular evidence that reconstituted gene expression in these lines is indeed a result of somatic recombination.

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Section: Conflicting Results From Shr Reporter Linescontrasting

confidence: 55%

Section: Somatic Homologous Recombination Reporter Lines: Lack Of Sufmentioning

confidence: 99%

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

et al. 2012

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“…These can be exploited as bioindicators of environmental pollution (Kovalchuk et al, 1998(Kovalchuk et al, , 2001a(Kovalchuk et al, , 2001b or as monitors of mutation frequencies resulting from mutant backgrounds or stress conditions in a laboratory setting (Lebel et al, 1993;Puchta et al, 1995;Kovalchuk et al, 2000Kovalchuk et al, , 2001bKovalchuk et al, , 2003Lucht et al, 2002;Leonard et al, 2003;Hoffman et al, 2004;Yoshihara et al, 2006). They provide either a direct or indirect way to determine the number of certain types of genome alterations.…”

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confidence: 99%

Development and Application of Novel Constructs to Score C:G-to-T:A Transitions and Homologous Recombination in Arabidopsis

et al. 2007

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We report on the development of five missense mutants and one recombination substrate of the b-glucuronidase (GUS)-encoding gene of Escherichia coli and their use for detecting mutation and recombination events in transgenic Arabidopsis (Arabidopsis thaliana) plants by reactivation of GUS activity in clonal sectors. The missense mutants were designed to find C:Gto-T:A transitions in a symmetrical sequence context and are in that respect complementary to previously published GUS point mutants. Small peptide tags (hemagglutinin tag and Strep tag II) and green fluorescent protein were translationally fused to GUS, which offers possibilities to check for mutant GUS production levels. We show that spontaneous mutation and recombination events took place. Mutagenic treatment of the plants with ethyl methanesulfonate and ultraviolet-C increased the number of mutations, validating the use of these constructs to measure mutation and recombination frequencies in plants exposed to biotic or abiotic stress conditions, or in response to different genetic backgrounds. Plants were also subjected to heavy metals, methyl jasmonate, salicylic acid, and heat stress, for which no effect could be seen. Together with an ethyl methanesulfonate mutation induction level much higher than previously described, the need is illustrated for many available scoring systems in parallel. Because all GUS missense mutants were cloned in a bacterial expression vector, they can also be used to score mutation events in E. coli.

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Heavy metals are highly toxic for living organisms when excess amounts exist in the environment (Salt et al 1998;Kovalchuk et al 2001). Soils and water contaminated with heavy metals cause major environmental and human health problems.

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confidence: 99%

“…During the last few decades, phytoremediation and plant-based assays for cleanup and monitoring of these contaminated environments have been developed (Salt et al 1998;Kovalchuk et al 2001;Krizek et al 2003;Pilon-Smits 2005). These methods take advantage of the sedentary nature of plants, providing inexpensive and nontechnical means of environmental analysis and restoration (Kovalchuk et al 2001;Krizek et al 2003).Among heavy metals, selenium and chromium function as toxic analogs of sulfur, and they exist in nature mainly as selenate (SeO 4 …”

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confidence: 99%

Sulfur-responsive promoter of sulfate transporter gene is potentially useful to detect and quantify selenate and chromate

Maruyama‐Nakashita

Inoue

Saito

et al. 2007

Plant Biotechnology

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Heavy metals are highly toxic for living organisms when excess amounts exist in the environment (Salt et al. 1998;Kovalchuk et al. 2001). Soils and water contaminated with heavy metals cause major environmental and human health problems. During the last few decades, phytoremediation and plant-based assays for cleanup and monitoring of these contaminated environments have been developed (Salt et al. 1998;Kovalchuk et al. 2001;Krizek et al. 2003;Pilon-Smits 2005). These methods take advantage of the sedentary nature of plants, providing inexpensive and nontechnical means of environmental analysis and restoration (Kovalchuk et al. 2001;Krizek et al. 2003).Among heavy metals, selenium and chromium function as toxic analogs of sulfur, and they exist in nature mainly as selenate (SeO 4 2Ϫ) and chromate (CrO 4 2Ϫ ) anions at ϩ6 oxidation states. Because of their structural similarities to sulfate (SO 4 2Ϫ ), selenate and chromate are readily imported into plant cells through the activities of sulfate transporters. In fact, selenate competitively inhibits the influx of sulfate, and after incorporation into the cells, it is subsequently assimilated into selenocysteine and seleno-methionine by sharing the pathways of sulfur metabolism; ultimately, these seleniumcontaining amino acids are incorporated into proteins (Terry et al. 2000;Ellis and Salt 2003). Chromate also has an inhibitory effect on sulfate uptake, but is mainly reduced to ϩ3 oxidation states, Cr 3ϩ or Cr 2 O 3 , presumably by using reducing cofactors. Accordingly, excess amounts of selenate and chromate taken up from the environment can affect the cellular function of living organisms and sometimes have lethal effects. Indeed, several mutants lacking sulfate transporter genes have been isolated from yeast and plants by using the toxic effects of selenate and chromate as sulfate analogs (Smith et al. 1995a, b;Cherest et al. 1997;Shibagaki et al. 2002).In Arabidopsis, two high-affinity sulfate transporters, SULTR1;1 and SULTR1;2, facilitate the initial uptake of sulfate into the roots (Takahashi et al. 2000;Vidmar et al. 2000;Shibagaki et al. 2002; Yoshimoto et al. 2002). These transporters are expressed in the epidermis and cortex of roots, and their transcripts can accumulate through sulfur depletion to maximize sulfate uptake for efficiently using limited amounts of sulfur in the soil environment (Takahashi et al. 2000;Vidmar et al. 2000;Shibagaki et al. 2002; Yoshimoto et al. 2002). The expression of these SULTRs depends on the promoter activities of their 5Ј-regions controlled by sulfur deficiency (Maruyama-Nakashita et al. 2004a, b, 2005. The presence of a specific sulfur-responsive cis-element in the SULTR1;1 promoter region further elaborates the Abstract Plant-based assays for monitoring contaminated environments provide inexpensive and nontechnical means of environmental analysis. Here we report a model system for monitoring selenium and chromium, which are highly toxic heavy metals for living organisms. The major forms of selenium and chromium ...

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A sensitive transgenic plant system to detect toxic inorganic compounds in the environment

Cited by 104 publications

References 30 publications

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

Development and Application of Novel Constructs to Score C:G-to-T:A Transitions and Homologous Recombination in Arabidopsis

Sulfur-responsive promoter of sulfate transporter gene is potentially useful to detect and quantify selenate and chromate

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