A sensitive HPLC-FL method to simultaneously determine febuxostat and diclofenac in rat plasma: assessment of metabolic drug interactions <i>in vitro</i> and <i>in vivo</i>

Han, Dong-Gyun; Kim, Kyu-Sang; Seo, Seong-Wook; Baek, Young Mee; Jung, Yunjin; Kim, Dae‐Duk; Yoon, In‐Soo

doi:10.1039/d0ay00471e

Cited by 13 publications

(7 citation statements)

References 40 publications

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“…The reported methods involve LC-MS/MS [10,11], HPLC-UV detection [12,13], CZE [14], and spectrofluorimetry [15,16]. Concerning FEB, a number of HPLC methods had been reported using photo diode array [8,17], fluorescence [18,19], and mass detection [20][21][22]. It was also investigated by high-performance TLC [23,24], GC [25], MEKC [26], and spectrofluorimetric methods [15,27].…”

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confidence: 99%

A novel quality by design approach for development and validation of a green reversed‐phase HPLC method with fluorescence detection for the simultaneous determination of lesinurad, febuxostat, and diflunisal: Application to human plasma

Magdy

Hakiem

Belal

et al. 2021

J of Separation Science

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A novel and eco‐friendly reversed‐phase HPLC method with fluorescence detection was developed for simultaneous estimation of two co‐administered antigout drugs (lesinurad and febuxostat) with diflunisal as a nonsteroidal anti‐inflammatory drug. Unlike routine methodology, the developed method was optimized using analytical quality by design approach. A full factorial design was applied to optimize the effect of variable factors on chromatographic responses. The chromatographic separation was performed using isocratic elution on the Hypersil BDS C18 column at 40°C. The mobile phase consisted of acetonitrile:potassium phosphate buffer (30.0 mM; pH 5.5, 32.2:67.8% v/v) pumped at a flow rate of 1.0 mL/min and injection volume of 20.0 μL was employed. The proposed method was able to separate the ternary mixture in <10 min. The calibration curves of diflunisal, lesinurad, and febuxostat were linear over concentration ranges of 50.0–500.0, 50.0–700.0, and 20.0–700.0 ng/mL, respectively. Recovery percentages ranging from 98.1 to 101.3% with % relative standard deviation of <2% were obtained upon spiking to human plasma samples, indicating high bioanalytical applicability. Furthermore, the method was found to be excellent green when it was assessed according to Green Analytical Procedure Index and analytical Eco‐Scale guidelines.

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confidence: 99%

A novel quality by design approach for development and validation of a green reversed‐phase HPLC method with fluorescence detection for the simultaneous determination of lesinurad, febuxostat, and diflunisal: Application to human plasma

Magdy

Hakiem

Belal

et al. 2021

J of Separation Science

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“…However, it appears that this result can be changed when either other dose levels vehicle systems, or both, are used, which warrants further investigation. Additionally, the total blood clearance (calculated as CL/R B ) of acacetin is 191 ± 34 mL/min/kg, which far exceeds the hepatic blood flow rate of 50–80 mL/min/kg in rats [ 12 , 22 ]. As the liver exhibited the highest metabolic activity among various organs ( Figure 5 ), it is speculated that acacetin is a drug with a high hepatic extraction ratio of nearly 1.…”

Section: Discussionmentioning

confidence: 99%

“…Eight-week-old male Sprague-Dawley rats weighing approximately 250 g were purchased from DBL Co. (Chungcheongbuk-do, Korea). The rats were housed and acclimatized before use, as reported previously [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Investigation of the Factors Responsible for the Poor Oral Bioavailability of Acacetin in Rats: Physicochemical and Biopharmaceutical Aspects

et al. 2021

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Acacetin, an important ingredient of acacia honey and a component of several medicinal plants, exhibits therapeutic effects such as antioxidative, anticancer, anti-inflammatory, and anti-plasmodial activities. However, to date, studies reporting a systematic investigation of the in vivo fate of orally administered acacetin are limited. Moreover, the in vitro physicochemical and biopharmaceutical properties of acacetin in the gastrointestinal (GI) tract and their pharmacokinetic impacts remain unclear. Therefore, in this study, we aimed to systematically investigate the oral absorption and disposition of acacetin using relevant rat models. Acacetin exhibited poor solubility (≤119 ng/mL) and relatively low stability (27.5–62.0% remaining after 24 h) in pH 7 phosphate buffer and simulated GI fluids. A major portion (97.1%) of the initially injected acacetin dose remained unabsorbed in the jejunal segments, and the oral bioavailability of acacetin was very low at 2.34%. The systemic metabolism of acacetin occurred ubiquitously in various tissues (particularly in the liver, where it occurred most extensively), resulting in very high total plasma clearance of 199 ± 36 mL/min/kg. Collectively, the poor oral bioavailability of acacetin could be attributed mainly to its poor solubility and low GI luminal stability.

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“…From the literature survey, the analysis of FEB was performed using different methods including spectrofluorimetry [10][11][12][13], spectrophotometry [14,15], HPLC [9,[16][17][18][19], LC-MS [20][21][22], micellar electrokinetic capillary chromatography [23], HPTLC [24][25][26][27] and GC [28]. Similarly, the assay of IBU has been achieved applying different methods including spectrofluorimetry [29][30][31][32], spectrophotometry [33,34], HPLC [35][36][37], LC-MS [38,39], HPTLC [37], capillary zone electrophoresis [40,41], potentiometry [42] and GC [43,44].…”

Section: Introductionmentioning

confidence: 99%

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

et al. 2021

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Two green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respectively, using Δ λ of 40 nm. Method II is constant-energy synchronous spectrofluorimetry using a wavenumber interval of −4000 cm −1 . All measurements were carried out in a borate buffer of pH 7 and distilled water for dilution which increased the methods' greenness. The two methods were rectilinear over concentration ranges of 30.0–700.0 ng ml −1 and 0.5–9.0 µg ml −1 in the first method and 20.0–500.0 ng ml −1 and 0.1–8.0 µg ml −1 in the second method for FEB and IBU, respectively. High sensitivity was attained for the two drugs with limits of quantitations (LODs) down to 0.41 and 5.51 ng ml −1 in the first method and 0.25 and 3.32 ng ml −1 in the second method for FEB and IBU, respectively. Recovery percentages were in the range of 97.3–101.9% after extraction from spiked human plasma samples, demonstrating high bioanalytical applicability. The two methods were further applied to tablet dosage forms with good recovery results. The methods' greenness was assessed according to the analytical Eco-Scale and Green Analytical Procedure Index guidelines.

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A sensitive HPLC-FL method to simultaneously determine febuxostat and diclofenac in rat plasma: assessment of metabolic drug interactions in vitro and in vivo

Abstract: We developed a sensitive, simple and validated HPLC-FL method for simultaneous determination of FEB and DIC in rat plasma. The method requires a relatively small volume of sample, has simple sample preparation and excellent sensitivity.

Cited by 13 publications

References 40 publications

A novel quality by design approach for development and validation of a green reversed‐phase HPLC method with fluorescence detection for the simultaneous determination of lesinurad, febuxostat, and diflunisal: Application to human plasma

A novel quality by design approach for development and validation of a green reversed‐phase HPLC method with fluorescence detection for the simultaneous determination of lesinurad, febuxostat, and diflunisal: Application to human plasma

Investigation of the Factors Responsible for the Poor Oral Bioavailability of Acacetin in Rats: Physicochemical and Biopharmaceutical Aspects

Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

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