A self-contained microfluidic in-gel loop-mediated isothermal amplification for multiplexed pathogen detection

Chen, Chen; Liu, Pian; Zhao, Xing; Du, Wei; Feng, Xiaojun; Liu, Bi‐Feng

doi:10.1016/j.snb.2016.07.164

Cited by 52 publications

(30 citation statements)

References 40 publications

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“…Microfluidic technology is a new approach of POC that can overcome the limitation of conventional POC by providing improvements of fewer reagents consumption, increased portability, simplicity, and reduced reaction time [61]. It involves the preloading of the master mix containing all the reaction components in microliter volumes into a sample loading well where it moves into the reaction chamber and the amplicon detected at another segment of the setup.…”

Section: Microfluidic Platformmentioning

confidence: 99%

“…It involves the preloading of the master mix containing all the reaction components in microliter volumes into a sample loading well where it moves into the reaction chamber and the amplicon detected at another segment of the setup. Many studies reported different setup, for example, Chen et al [61] used in-gel microfluidic LAMP for multiplex detection of foodborne pathogens. Chung et al [62] designed a microfluidic chip module for detecting murine norovirus in oysters using charge switchable micro-bead beating.…”

Section: Microfluidic Platformmentioning

confidence: 99%

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Lassa Virus Diagnostic Platforms: Limitations and Prospects

Hassan

Kumurya

Aminu

et al. 2020

AJRID

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Background: Lassa virus (LASV) is the cause of lassa fever (LF) belonging to the Arenaviridae family. Clinical diagnosis is often difficult because of symptoms commonality with other infectious diseases. Early and rapid diagnosis is critical for therapy initiation and LF transmission prevention and control. Aims: This review aims to highlight current diagnostic platforms and prospects of new emerging sensitive platforms. Methodology: Available published articles on LASV diagnostics with a focus on current methods: virus culture, enzyme-linked immunosorbent assay (ELISA), reverse transcriptase-polymerase chain reaction (RT – PCR) and rapid diagnostic tests (RDT) were reviewed based on their performances and limitations. Prospects of new diagnostic platforms: mobile health, microfluidic, clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas), Loop-mediated isothermal amplification (LAMP) for LASV diagnosis were also reviewed. Results: Low sensitivity of the ELISA platform during the window period of LASV infection was observed. Moreover, RT – PCR findings indicated limitation of expertise necessity, cost of thermal cycler, and dedicated facility. Molecular-based point-of-care (POC) diagnostic development should be prioritized to increase speed and sensitivity. Conclusion: The integration of POC device into molecular isothermal method against LASV scourge will be a success story in curving intermittent outbreaks in endemic areas and prompt clinical management.

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Section: Microfluidic Platformmentioning

confidence: 99%

Section: Microfluidic Platformmentioning

confidence: 99%

Lassa Virus Diagnostic Platforms: Limitations and Prospects

Hassan

Kumurya

Aminu

et al. 2020

AJRID

View full text Add to dashboard Cite

show abstract

“…To facilitate their deployment in POC settings, LAMP reagents can be stored in dried form with a shelf life of up to 30 days at 56 °C [ 151 ]. Chen et al [ 152 ] were able to store reagents within a low-melting-point agarose gel maintained at 4 °C for long-term storage.…”

Section: Amplificationmentioning

confidence: 99%

“…The sensitivity of the multiplex device was found to be 100 orders of magnitude better than typical PCR and is comparable to the sensitivity of real-time PCR. Chen [ 152 ] also demonstrated multiplex detection of four bacterial DNA targets with a detection sensitivity of three copies/μL with agarose gel prestoring.…”

Section: Amplificationmentioning

confidence: 99%

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Basha

Yousuff

et al. 2017

Micromachines

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Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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“…It takes up to one week to determine the specific pathogen microorganisms. Therefore, different methods of pathogen detections are in high demand in the food industry to avoid the spread of any bacterial diseases from food poisoning [ 18 , 19 ]. The detection methods can be categorized into the following groups: conventional methods and various sensor-based methods.…”

Section: Introductionmentioning

confidence: 99%

Detection Methodologies for Pathogen and Toxins: A Review

Alahi

Mukhopadhyay

2017

Sensors

140

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Pathogen and toxin-contaminated foods and beverages are a major source of illnesses, even death, and have a significant economic impact worldwide. Human health is always under a potential threat, including from biological warfare, due to these dangerous pathogens. The agricultural and food production chain consists of many steps such as harvesting, handling, processing, packaging, storage, distribution, preparation, and consumption. Each step is susceptible to threats of environmental contamination or failure to safeguard the processes. The production process can be controlled in the food and agricultural sector, where smart sensors can play a major role, ensuring greater food quality and safety by low cost, fast, reliable, and profitable methods of detection. Techniques for the detection of pathogens and toxins may vary in cost, size, and specificity, speed of response, sensitivity, and precision. Smart sensors can detect, analyse and quantify at molecular levels contents of different biological origin and ensure quality of foods against spiking with pesticides, fertilizers, dioxin, modified organisms, anti-nutrients, allergens, drugs and so on. This paper reviews different methodologies to detect pathogens and toxins in foods and beverages.

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A self-contained microfluidic in-gel loop-mediated isothermal amplification for multiplexed pathogen detection

Cited by 52 publications

References 40 publications

Lassa Virus Diagnostic Platforms: Limitations and Prospects

Lassa Virus Diagnostic Platforms: Limitations and Prospects

Towards Multiplex Molecular Diagnosis—A Review of Microfluidic Genomics Technologies

Detection Methodologies for Pathogen and Toxins: A Review

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