A Self-Assembled Ratiometric Polymeric Nanoprobe for Highly Selective Fluorescence Detection of Hydrogen Peroxide

Abstract

In this study, a dual-emission fluorescence resonance energy transfer (FRET) polymeric nanoprobe by single-wavelength excitation was developed for sensitive and selective hydrogen peroxide (HO) detection. Polymeric nanoprobe was prepared by simple self-assembly of functional lipopolymers, which were 4-carboxy-3-fluorophenylboronic acid (FPBA)-modified DSPE-PEG (DSPE-PEG-FPBA) and 7-hydroxycoumarin (HC)-conjugated DSPE-PEG (DSPE-PEG-HC). Subsequent binding of alizarin red S (ARS) to FPBA endowed the nanoprobe with a new fluorescence emission peak at around 600 nm. Because of the perfect match of the fluorescence emission spectra of HC with the absorbance spectra of ARS-FPBA, FRET was achieved between them. The sensing strategy for HO was based on HO-induced deboronation reaction and boronic acid-mediated ARS fluorescence. Interaction between phenylboronic acid and ARS was revisited herein and it was found that electron-donating or -withdrawing group on phenylboronic acid (PBA) has significant influence on the fluorescence property of ARS, which enabled sensitive and selective HO sensing. The nanoprobe displayed two well-separated emission bands (150 nm), providing high specificity and sensitivity for ratiometric detection of HO. Further application was exploited for the determination of glucose and the results demonstrated that the proposed strategy showed ratiometric response capability for glucose detection. The current method does not involve complicated organic synthesis and opens a new avenue for the construction of multifunctional polymeric fluorescent nanoprobe.

Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H2O2 biosensor applications

“…Therefore, sensitive and reliable detection of H2O2 is drawing much attention in numerous fields. Over the past decades, a wide range of sensitive methods has been used for reliable detection of H2O2 such as fluorescence, chemiluminescence, chromatography, spectrophotometry and electrochemical techniques (Feng, Wang, Dang, Xu, Yu, & Zhang, 2017.;Yuan & Shiller, 1999;Almuaibed & Townshend, 1994;Sherino, Mohamad, Nadiah Halim, Suhana & Manan, 2018.). Compared to available traditional chromatographic, spectrophotometric and fluorescence methods, the electrochemical techniques are widely used for the detection of H2O2 due to their high sensitivity, portability, and cost-effectiveness (Rismetov, Ivandini, Saepudin, & Einaga, 2014.).…”

Water‐Soluble Polymeric Probes for the pH‐Tunable Fluorometric Detection of Hydrogen Peroxide

“…A good linear relationship was observed between the (I 0 / I) and concentration of H 2 O 2 ; the detection limit of P2 to H 2 O 2 was calculated to be 0.18 M (Figure S8). Due to the bulky polymer probes, the sensitivity of this system is not good as reported previously …”

“…Hydrogen peroxide (H 2 O 2 ) is a reactive oxygen species (ROS) that is used widely in many areas, including food manufacturing applications, industrial chemical, clinical, industrial wastewater and effluent treatment, textiles, bleaching of wood pulp and paper, mining, and pharmaceutical industry . In addition, H 2 O 2 plays an essential role in many biological systems, such as signal transduction, immune‐cell activation, cell differentiation, apoptosis, vascular re‐modeling, stomatal closure, and root growth . Therefore, the efficient detection of H 2 O 2 is highly desirable.…”

“…Compared to other methods, the fluorometric detection method has several advantages, including high selectivity, good sensitivity, invasiveness, speed, operational simplicity, and useful applications in chemistry, biology, and medicine . The design strategies are based on a specific reaction between recognition groups, such as boronate groups (boronic acid or boronate ester), and H 2 O 2 , which give products with different photophysical properties .…”

Development of Ratiometric Fluorescence Sensors Based on CdSe/ZnS Quantum Dots for the Detection of Hydrogen Peroxide

“…Therefore, the detection of H 2 O 2 and its control in each field are needed for safe human life. Among numerous analytical methods published for the detection of H 2 O 2 , fluorescence methods are the most preferred ones due to their high sensitivity and selectivity [1,10]. Many fluorescence probes showed strong affinity to react with H 2 O 2 and they were applied for H 2 O 2 detection in living cells [11,12,13,14,15].…”