1992
DOI: 10.1128/jvi.66.6.3677-3682.1992
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Abstract: Several monoclonal antibodies (MAbs) raised against hog cholera virus (HCV) reacted with the HCV structural glycoprotein gp44/48 and neutralized the virus. The presence of HCV gp44/48 on the viral surface was directly demonstrated by immunogold electron microscopy. Eight anti-HCV gp44/48 MAbs were tested by immunoperoxidase assay against a panel of pestivirus strains. Each MAb showed a distinct pattern of reactivity with HCV * Corresponding author.

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Cited by 141 publications
(67 citation statements)
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References 26 publications
(67 reference statements)
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“…The following antibodies were used for detection of the proteins: for E rns , rabbit antiserum BaculoE0 (kindly provided by Jürgen Thiel and Robert Stark, Universität Giessen) or mouse monoclonal 24/16 (59); for the ER Golgi apparatus intermediate compartment (ERGIC), mouse monoclonal antibody against ERGIC-53 (Enzo Life Sciences/Alexis Biochemicals, Lörrach, Germany); for Golgi, mouse monoclonal antibody against 58K protein (GeneTex, San Antonio, TX, USA); for ER, mouse monoclonal antibody against calregulin (Abcam, Cambridge, UK); and for TRITC, labeled goat anti-rabbit (Abcam).…”
Section: Immunofluorescencementioning
confidence: 99%
“…While the glycoproteins E1 and E2 have their counterparts in other members of the Flaviviridae family, E rns is unique to the pestivirus genus (Lindenbach et al, 2007). It is essential for production of infectious viral particles and is targeted by neutralizing antibodies (Weiland et al, 1992;Windisch et al, 1996). E rns was also shown to play a role in virus attachment to cells via a positively charged amino acid stretch that has been suggested to interact with glycosaminoglycans such as heparan sulfate Iqbal et al, 2000;Iqbal and McCauley, 2002).…”
Section: Introductionmentioning
confidence: 99%
“…The envelope glycoproteins E rns and E2 are involved in CSFV infection. E rns and E2 are present on the outer surface of the virion (6,7) and are recognized to be the main targets for neutralizing antibodies (6,8). They are thus inferred to be responsible for the attachment and entry of CSFV.…”
mentioning
confidence: 99%
“…E rns , initially termed E0, was first detected at the surface of pestiviral particles and shortly thereafter also in the supernatant of virus infected cells [60,61]. Accordingly, E rns was detected in vivo with concentrations up to 50 ng/ml in the serum of PI animals [29].…”
Section: E Rns As Ifn Antagonistmentioning
confidence: 99%
“…Antibodies directed against Erns and E2 have been demonstrated in infected animals (18)(19)(20)(21). Monoclonal antibodies to Erns (22) or E2 (23, 24) can neutralize virus infectivity, and both proteins can confer protective immu nity to pigs (25)(26)(27). The antigenic sites or regions of the pestivirus…”
mentioning
confidence: 99%