A role of <i>miR‐27</i> in the regulation of adipogenesis

Lin, Qun; Gao, Zhan‐Guo; Alarcón, Rodolfo M.; Ye, Jianping; Yun, Zhong

doi:10.1111/j.1742-4658.2009.06967.x

Cited by 407 publications

(356 citation statements)

References 33 publications

(61 reference statements)

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“…111 The expression of miR-27 decreases during adipogenesis and, interestingly, was found to be increased in adipose tissue of obese mouse. Finally, significant correlation of the levels of differents miRNAs with metabolic parameters such as fasting plasma glucose or circulating leptin and adiponectin has also been reported, 112 highlighting the importance of these factors in the regulation of metabolism.…”

Section: Differentiation Of Preadipocytesmentioning

confidence: 99%

A chromatin perspective of adipogenesis

2010

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Section: Differentiation Of Preadipocytesmentioning

confidence: 99%

A chromatin perspective of adipogenesis

2010

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“…It has been reported that miR-27 enhances differentiation of osteoblasts and strongly inhibits adipogenesis (Lin et al, 2009;Wang and Xu, 2010). MiR-27b is a regulatory hub in lipid metabolism through repressing critical regulators of lipid homeostasis, such as PPARα, HMGCR (Kida et al, 2011, Vickers et al, 2013.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

Yang

Chen

Huang

et al. 2014

Animal

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MicroRNAs are endogenous~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3′-untranslated region (3′UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3′UTR in mouse. In this study, we cloned the full-length 3′UTR of porcine myostatin by rapid amplification of 3′-cDNA ends (3′-RACE) and demonstrated that the 3′UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.

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“…또한, miR-26도 지방분화를 촉진한다고 알려져 있다 [14]. 이와는 반대로, let-7a와 miR-27은 C/EBPα와 PPARγ의 발현을 제어하여 지방분화를 억제시킨다 [11,15] (Fig. 1A).…”

Section: 서 론unclassified

Transcriptional Regulation of MicroRNA-17 by PPARγ in Adipogenesis

Bae¹,

Kim²,

Chung³

et al. 2014

Journal of Life Science

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MicroRNAs comprise a family of small noncoding RNAs that modulate physiological processes, including adipogenesis. promotes adipocyte differentiation and enhances lipid accumulation. The transcriptional regulation of miR-17 during adipogenesis remains unknown. In this study, we investigated whether miR-17 is a target of peroxisome proliferator-activated receptor γ (PPARγ), which is a key regulator of adipogenesis. The levels of miR-17 and the expression of PPARγ increased after the induction of adipocyte differentiation. Three putative peroxisome proliferator response elements (PPREs) were identified in the miR-17 promoter region. Using chromatin immunoprecipitation and luciferase reporter assays, we observed the interaction of PPARγ with the miR-17 promoter. Mutagenesis experiments showed that the -677/-655 region of the miR-17 promoter could function as a PPRE site. These results suggest that PPARγ is essential for transcriptional activation of the miR-17 gene, thereby contributing to understanding the molecular mechanism of adipogenesis in adipocytes.Key words : Adipogenesis, microRNA, peroxisome proliferator-activated receptor γ, 3T3-L1 cells *Corresponding author *Tel : +82-2-961-9208, Fax : +82-2-964-1079 *E-mail : shkim@khu.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A role of miR‐27 in the regulation of adipogenesis

Cited by 407 publications

References 33 publications

A chromatin perspective of adipogenesis

A chromatin perspective of adipogenesis

MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

Transcriptional Regulation of MicroRNA-17 by PPARγ in Adipogenesis

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