A role for the regulator PsrA in the polyhydroxyalkanoate metabolism of Pseudomonas putida KT2440

Fonseca, Pilar; Peña, Fernando de la; Prieto, María Auxiliadora

doi:10.1016/j.ijbiomac.2014.04.014

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…P . putida S12 does not harbour a fadR homologue, but the gene psrA is responsible for the regulation of part of the fatty acid degradation pathway [ 18 , 19 ]. In toluene-exposed P .…”

Section: Resultsmentioning

confidence: 99%

Dynamic Response of Pseudomonas putida S12 to Sudden Addition of Toluene and the Potential Role of the Solvent Tolerance Gene trgI

et al. 2015

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Pseudomonas putida S12 is exceptionally tolerant to various organic solvents. To obtain further insight into this bacterium’s primary defence mechanisms towards these potentially harmful substances, we studied its genome wide transcriptional response to sudden addition of toluene. Global gene expression profiles were monitored for 30 minutes after toluene addition. During toluene exposure, high oxygen-affinity cytochrome c oxidase is specifically expressed to provide for an adequate proton gradient supporting solvent efflux mechanisms. Concomitantly, the glyoxylate bypass route was up-regulated, to repair an apparent toluene stress-induced redox imbalance. A knock-out mutant of trgI, a recently identified toluene-repressed gene, was investigated in order to identify TrgI function. Remarkably, upon addition of toluene the number of differentially expressed genes initially was much lower in the trgI-mutant than in the wild-type strain. This suggested that after deletion of trgI cells were better prepared for sudden organic solvent stress. Before, as well as after, addition of toluene many genes of highly diverse functions were differentially expressed in trgI-mutant cells as compared to wild-type cells. This led to the hypothesis that TrgI may not only be involved in the modulation of solvent-elicited responses but in addition may affect basal expression levels of large groups of genes.

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“…P . putida S12 does not harbour a fadR homologue, but the gene psrA is responsible for the regulation of part of the fatty acid degradation pathway [ 18 , 19 ]. In toluene-exposed P .…”

Section: Resultsmentioning

confidence: 99%

Dynamic Response of Pseudomonas putida S12 to Sudden Addition of Toluene and the Potential Role of the Solvent Tolerance Gene trgI

et al. 2015

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“…In general, the presented results indicated the difference in the transcriptional response to nitrogen limitation during microbial mcl-PHAs synthesis. Although Pseudomonas putida KT2440 has been used as a model microorganism in different studies including those looking at molecular machinery [5, 8, 13, 39], its nitrogen and substrate response transcriptome has not yet been analyzed and compared. To better understand the process, a time course study of the Pseudomonas putida KT2440 response transcriptome was carried out.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

Możejko-Ciesielska

Pokój

Ciesielski

2018

Journal of Industrial Microbiology and Biotechnology

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Bacterial response to environmental stimuli is essential for survival. In response to fluctuating environmental conditions, the physiological status of bacteria can change due to the actions of transcriptional regulatory machinery. The synthesis and accumulation of polyhydroxyalkanoates (PHAs) are one of the survival strategies in harsh environments. In this study, we used transcriptome analysis of Pseudomonas putida KT2440 to gain a genome-wide view of the mechanisms of environmental-friendly biopolymers accumulation under nitrogen-limiting conditions during conversion of metabolically different carbon sources (sodium gluconate and oleic acid). Transcriptomic data revealed that phaG expression is associated with medium-chain-length-PHAs’ synthesis not only on sodium gluconate but also on oleic acid, suggesting that PhaG may play a role in this process, as well. Moreover, genes involved in the β-oxidation pathway were induced in the PHAs production phase when sodium gluconate was supplied as the only carbon and energy source. The transition from exponential growth to stationary phase caused a significant expression of genes involved in nitrogen metabolism, energy supply, and transport system. In this study, several molecular mechanisms, which drive mcl-PHAs synthesis, have been investigated. The identified genes may provide valuable information to improve the efficiency of this bioprocess and make it more economically feasible.Electronic supplementary materialThe online version of this article (10.1007/s10295-018-2042-4) contains supplementary material, which is available to authorized users.

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“…In P. putida , the nature of the substrate used as carbon source and PHA precursor has an impact on PHA metabolism. Fatty acids, which are structurally related to PHAs, are incorporated into them during the exponential phase of growth, driven by the global regulator PsrA (Fonseca et al ., ). In contrast, a biomass build‐up phase is required before structurally unrelated compounds such as carbohydrates, glycerol and aromatic molecules become involved in PHA accumulation.…”

Section: Metabolic and Regulatory Network Wiring The Pha Cycle A Crmentioning

confidence: 97%

“…This extends the role of the Crc protein in the optimization of metabolism beyond that of the hierarchical utilization of carbon sources, a facet that has not been studied in other regulatory circuits (La Rosa et al ., ). Other global transcriptional factors such as RpoS, PsrA and the GacS/GacA system are involved in the regulation of PHA production (Ruiz et al ., ; de Eugenio, ; Fonseca et al ., ). A reduction in PHA content linked to GacS sensor kinase disruption has been seen in the CA‐3 and KT2442 strains of P. putida (de Eugenio, ; Ryan et al ., ).…”

Section: Metabolic and Regulatory Network Wiring The Pha Cycle A Crmentioning

confidence: 97%

“…By controlling the carbon flow through the β‐oxidation pathway, PHAs can be rationally designed to have shorter or longer monomer chain lengths [e.g. by deleting the repressor PsrA (Fonseca et al ., ) and by slowing down the β‐oxidation route respectively] (Olivera et al ., ; Liu et al ., ). A β‐oxidation‐impaired mutant, with deletions concerning the last two steps of this pathway (i.e.…”

Section: Systemic Metabolic Modulation: Broadening the Pha Applicatiomentioning

confidence: 99%

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A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida

Prieto

Escapa

Martínez

et al. 2015

Environmental Microbiology

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A role for the regulator PsrA in the polyhydroxyalkanoate metabolism of Pseudomonas putida KT2440

Cited by 20 publications

References 31 publications

Dynamic Response of Pseudomonas putida S12 to Sudden Addition of Toluene and the Potential Role of the Solvent Tolerance Gene trgI

Dynamic Response of Pseudomonas putida S12 to Sudden Addition of Toluene and the Potential Role of the Solvent Tolerance Gene trgI

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida

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