A role for the collagen I/III and MMP-1/-13 genes in primary inguinal hernia?

Abstract

BackgroundAbnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered collagen metabolism and structural changes of the tissue in patients with primary inguinal hernia. However, it is still unknown whether these alterations reflect a basic dysfunction of the collagen synthesis, or of collagen degradation.MethodsIn the present study, we analysed type I and type III procollagen messenger ribonucleic acid (mRNA) and MMP-1 and MMP-13 mRNA in cultured fibroblasts from the skin of patients with primary inguinal hernia, and from patients without hernia (controls) by reverse transcription polymerase chain reaction (RT-PCR) and Northern Blot.ResultsThe results indicated that the ratio of type I to type III procollagen mRNA was decreased in patients with primary hernia, showing significant differences as compared to controls (p = 0.01). This decrease was mainly due to the increase of type III procollagen mRNA. Furthermore, RT-PCR analysis revealed that the expression of MMP-1 mRNA in patients with primary hernia is equivalent to that of controls (p > 0.05). In addition, MMP-13 mRNA is expressed neither in patients with primary hernia nor in controls.ConclusionWe concluded that abnormal change of type I and type III collagen mRNAs contribute to the development of primary inguinal hernia, whereas the expressions of MMP-1 and MMP-13 mRNA appears not to be involved in the development of primary inguinal hernia. Thus, the knowledge on the transcriptional regulation of collagen in patients with primary inguinal hernia may help to understand the pathogenesis of primary inguinal hernia, and implies new therapeutic strategies for this disease.

Quantitative and qualitative analysis of collagen types in the fascia transversalis of inguinal hernia patients

“…The genetic expression of collagen type I and III in fibroblasts derived from skin was studied by ROSH et al (23) . The measuring was carried out using the Reverse Transcriptase Polymerase Chain Reaction technique to measure the messenger RNA of the procollagen chain and it also obtained a diminished proportion of collagen types I and III.…”

Estudio de fibras colágenas y elásticas del tejido conjuntivo de pacientes con y sin hernia inguinal primaria

“…El gran reparo de este artículo es que fue también un estudio in vitro de síntesis proteica y expresión génica de los colágenos I y III que no ha sido repetido in vivo. Los hallazgos anteriormente descritos han sido confirmados recientemente, pues Rosh y cols 8 , estudiaron la expresión génica de los colágenos I/III en fibroblastos derivados de piel de 5 pacientes con hernia versus 5 controles sin hernia y edad promedio de 58 y 61 años. La medición fue realizada con técnica de RT-PCR para medir RNAm para cadenas de procolágeno, obteniendo también una disminución de la proporción de colágeno I/III.…”

“…Por otra parte, existen pocos estudios histoló-gicos que demuestren alteraciones en las fibras del tejido conjuntivo de pacientes con hernia inguinal primaria, pero parece ser que su importancia como factor etiológico es mayor del que se estimaba [4][5][6][7][8][9] . En relación a ello, nos planteamos si existiría alteración de las fibras elásticas y coláge-nas del tejido conjuntivo de pacientes portadores de hernia inguinal primaria operados en nuestro servicio al compararlos con un grupo de sujetos sin hernia, empleando histoquímica convencional en el tejido conectivo de pacientes con y sin hernia inguinal primaria para determinar las características (grosor y densidad) de fibras elásticas y colágenas de pacientes con hernia inguinal primaria y compararlos con sujetos sin hernia inguinal.…”

Técnica de Lichtenstein sob anestesia local em herniorrafias inguinais

“…O atual papel da falha do metabolismo do colágeno na patogenia das hérnias inguinais tem levado à nova compreensão da patologia e das causas de insucessos nos procedimentos cirúrgicos 34 . O defeito do metabolismo do colágeno leva a enfraquecimento do tecido fibroconectivo e, por conseguinte, ao desenvolvimento das hérnias inguinais.…”