A robust model of adjuvant-induced chronic unilateral arthritis in two mouse strains

Gauldie, Stephan D.; McQueen, Daniel S.; Clarke, Christopher J.; Chessell, Iain P.

doi:10.1016/j.jneumeth.2004.05.003

Cited by 50 publications

(51 citation statements)

References 25 publications

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“…Rheumatoid arthritis (RA), a common autoimmune disease, is characterized by chronic inflammation of synovial joints and progressive destruction of articular tissue (1). Cartilage destruction appears as narrowing of the joint space and is followed by destruction of the underlying bone (2,3). The disease is thought to affect approximately 1% of the population worldwide (2).…”

Section: Introductionmentioning

confidence: 99%

“…Cartilage destruction appears as narrowing of the joint space and is followed by destruction of the underlying bone (2,3). The disease is thought to affect approximately 1% of the population worldwide (2). The most commonly prescribed medication for rheumatoid arthritis is steroidal, nonsteroidal anti-inflammatory, disease-modifying anti-rheumatic, and immunosuppressant drugs (4).…”

Section: Introductionmentioning

confidence: 99%

“…Adjuvant-induced arthritis model was established for mice (2,8,9). Briefly, the animals are injected intradermally with FCA, which is composed of heat-killed Mycobacterium tuberculosis, suspended in paraffin oil, either in the tail base (10)(11)(12) or in the hind paw [plantar (13) or subplantar regions (14), tibiotarsal joint (9), or stifle joint (2)].…”

Section: Introductionmentioning

confidence: 99%

“…Briefly, the animals are injected intradermally with FCA, which is composed of heat-killed Mycobacterium tuberculosis, suspended in paraffin oil, either in the tail base (10)(11)(12) or in the hind paw [plantar (13) or subplantar regions (14), tibiotarsal joint (9), or stifle joint (2)]. Early manifestations that follow injection of FCA in the foot pads appear as swelling and edema, followed by inflammatory cell infiltration.…”

Section: Introductionmentioning

confidence: 99%

“…Hyperplasia of synovial lining cells and proliferation of granulation tissue take place later. This may be followed by damage of bone and cartilage, accompanied by rapid new bone formation in the adjacent periosteum (2,8,15).…”

Section: Introductionmentioning

confidence: 99%

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Prednisolone-Loaded PLGA Microspheres. In Vitro Characterization and In Vivo Application in Adjuvant-Induced Arthritis in Mice

et al. 2010

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Abstract. This study aimed at preparation of a sustained-release steroidal treatment for chronic inflammatory conditions, such as rheumatoid arthritis. To achieve such a goal, biodegradable polylactide-co-glycolide prednisolone-loaded microspheres were prepared using o/w emulsion solvent evaporation method. Formulation parameters were adjusted so as to optimize the microsphere characteristics. The prepared microspheres exhibited smooth and intact surfaces, with average size range not exceeding 65 µm. The encapsulation efficiency percent of most microsphere formulations fell within the range of 25-68%. Drug release from these microspheres took place over 4 weeks, with nearto-zero-order patterns. Two successful formulations were chosen for the treatment of unilateral arthritis, induced in mice using Freund's complete adjuvant (FCA). Inflammatory signs of adjuvant arthritis included severe swelling of the FCA-injected limbs, in addition to many histopathological lesions. These included inflammatory cell infiltration, synovial hyperplasia, cartilage, and bone erosion. Parenteral administration of the selected formulae dramatically reduced the swelling of the FCA-injected limbs. In addition, histological examination revealed that the microsphere treatment protocol efficiently protected cartilages and bones of mice, injected with FCA initial and booster doses, from erosion. These results could not be achieved by a single prednisolone dose of 5 mg/kg.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prednisolone-Loaded PLGA Microspheres. In Vitro Characterization and In Vivo Application in Adjuvant-Induced Arthritis in Mice

et al. 2010

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Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Shi¹,

Wang²,

Prescott³

et al. 2011

Arthritis & Rheumatism

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Objective. Assessment of infection with helminth parasites in murine models of disease could identify antiinflammatory mechanisms that translate into treatments for arthritic disease. The aim of this study was to test the ability of infection with the tapeworm Hymenolepis diminuta to ameliorate Freund's complete adjuvant (CFA)-induced monoarthritis in mice.Methods. Mice received CFA with or without H diminuta, and knee swelling, pain, and measures of inflammation were assessed.Results. Injection of CFA resulted in rapid (within 24 hours) and sustained (lasting 20 days) knee swelling, a decreased pain threshold, increased blood flow to the knee, and increased production of tumor necrosis factor ␣ and interleukin-12p40 (IL-12p40). In mice that were infected with H diminuta 8 days prior to receiving CFA, the severity of arthritis was reduced as assessed by these indices of inflammation and infection 2 days after CFA injection and resulted in more rapid resolution of knee swelling. This antiarthritic effect required a viable infection and was dependent on adaptive immunity, because infection with H diminuta did not protect mice lacking T cells and B cells or the IL-4 receptor ␣ chain from CFA-induced inflammation. Interleukin-10 was of prime importance in the antiarthritic effect, because IL-10-knockout mice were not protected by infection, the antiarthritic effect was ablated by use of neutralizing IL-10 antibodies, and transfer of CD4؉ cells from infected wild-type mice but not IL-10-knockout mice significantly reduced CFAinduced knee swelling.Conclusion. In mice, the adaptive immune response to infection with H diminuta involves mobilization of IL-10, which has the concomitant advantage of dampening the innate immune responses that drive CFA-induced joint inflammation.

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Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint

Ghilardi

Freeman

Jiménez-Andrade

et al. 2012

Arthritis & Rheumatism

133

129

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Objective Many forms of arthritis are accompanied by significant chronic joint pain. Here we studied whether there is significant sprouting of sensory and sympathetic nerve fibers in the painful arthritic knee joint and whether nerve growth factor (NGF) drives this pathological reorganization. Methods A painful arthritic knee joint was produced by injection of complete Freund’s adjuvant (CFA) into the knee joint of young adult mice. CFA-injected mice were then treated systemically with vehicle or anti-NGF antibody. Pain behaviors were assessed and at 28 days following the initial CFA injection, the knee joints were processed for immunohistochemistry using antibodies raised against calcitonin gene-related peptide (CGRP; sensory nerve fibers), neurofilament 200 kDa (NF200; sensory nerve fibers), growth associated protein-43 (GAP43; sprouted nerve fibers), tyrosine hydroxylase (TH; sympathetic nerve fibers), CD31 (endothelial cells) or CD68 (monocytes/macrophages). Results In CFA-injected mice, but not vehicle-injected mice, there was a significant increase in the density of CD68+ macrophages, CD31+ blood vessels, CGRP+, NF200+, GAP43+, and TH+ nerve fibers in the synovium as well as joint pain-related behaviors. Administration of anti-NGF reduced these pain-related behaviors and the ectopic sprouting of nerve fibers, but had no significant effect on the increase in density of CD31+ blood vessels or CD68+ macrophages. Conclusions Ectopic sprouting of sensory and sympathetic nerve fibers occurs in the painful arthritic joint and may be involved in the generation and maintenance of arthritic pain.

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A robust model of adjuvant-induced chronic unilateral arthritis in two mouse strains

Cited by 50 publications

References 25 publications

Prednisolone-Loaded PLGA Microspheres. In Vitro Characterization and In Vivo Application in Adjuvant-Induced Arthritis in Mice

Prednisolone-Loaded PLGA Microspheres. In Vitro Characterization and In Vivo Application in Adjuvant-Induced Arthritis in Mice

Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Neuroplasticity of sensory and sympathetic nerve fibers in a mouse model of a painful arthritic joint

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