A revision of current data and views on membrane hydrolysis and transport in the mammalian small intestine based on a comparison of techniques of chronic and acute experiments: Experimental re-investigation and critical review

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

“…It means that surgery leads to increasing of the muscle tissue (possibly because of the partial replacing with the connective tissue as a result of operation). It comports to the data obtained by Ugolev et al [20,21]. …”

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

“…It means that surgery leads to increasing of the muscle tissue (possibly because of the partial replacing with the connective tissue as a result of operation). It comports to the data obtained by Ugolev et al [20,21]. …”

The active and passive components of glucose absorption in rat jejunum under low and high perfusion stress

“…The inference that both carrier‐mediated processes (SGLT1 and GLUT2) are diminished by anaesthesia and surgery in rat is strongly supported by the experiments described in Table 2 of Ugolev et al (1986). These workers measured rates of glucose absorption in chronic, unanaesthetised rats implanted with jejunal perfusion cannulae and compared them with rates measured in chronic, anaesthetised rats and acute, anaesthetised rats which had just undergone laparotomy (the same as in our experiments).…”

“…Thus in both our work and their work the ratio of the ‘passive’ to ‘active’ components was just over 2 in low stress perfusions. Table 7 of Ugolev et al (1986) also shows that phloridzin inhibits the rate of absorption of 110 m m glucose by 75 %; this value compares with the 71 % inhibition at 100 m m glucose (Kellett & Helliwell, 2000) and 79 % inhibition at 100 m m 3‐ O ‐methylglucose (Uhing & Kimura, 1995). Thus three different laboratories have obtained similar data in rather different ways over a 20 year time scale.…”

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

“…Parameters of the model with approximation in the form of a smooth surface are shown in Table 1. Their values were generally in the range of values that were determined previously in chronic experiments on rats [14,16]. The value of K t was between 0.2 -1.0 mM [2,5] and 2.0 -5.0 mmol/l [6,7,9].…”

“…Nevertheless, we [11,14,16] and other researchers [15] have previously shown that anesthesia and surgical trauma significantly impairs functional characteristics of the small intestine. The results of chronic experiments on nonanesthetized animals [11,14,16] have shown that values of the maximal rate of the secondary active glucose transport under physiological conditions are significantly higher than the former estimates, based on the acute experiments in vivo.…”

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

“…It means that surgery leads to increasing of the muscle tissue (possibly because of the partial replacing with the connective tissue as a result of operation). It comports to the data obtained by Ugolev et al [20,21]. …”

The active and passive components of glucose absorption in rat jejunum under low and high perfusion stress

“…The inference that both carrier‐mediated processes (SGLT1 and GLUT2) are diminished by anaesthesia and surgery in rat is strongly supported by the experiments described in Table 2 of Ugolev et al (1986). These workers measured rates of glucose absorption in chronic, unanaesthetised rats implanted with jejunal perfusion cannulae and compared them with rates measured in chronic, anaesthetised rats and acute, anaesthetised rats which had just undergone laparotomy (the same as in our experiments).…”

“…Thus in both our work and their work the ratio of the ‘passive’ to ‘active’ components was just over 2 in low stress perfusions. Table 7 of Ugolev et al (1986) also shows that phloridzin inhibits the rate of absorption of 110 m m glucose by 75 %; this value compares with the 71 % inhibition at 100 m m glucose (Kellett & Helliwell, 2000) and 79 % inhibition at 100 m m 3‐ O ‐methylglucose (Uhing & Kimura, 1995). Thus three different laboratories have obtained similar data in rather different ways over a 20 year time scale.…”

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

“…Parameters of the model with approximation in the form of a smooth surface are shown in Table 1. Their values were generally in the range of values that were determined previously in chronic experiments on rats [14,16]. The value of K t was between 0.2 -1.0 mM [2,5] and 2.0 -5.0 mmol/l [6,7,9].…”

“…Nevertheless, we [11,14,16] and other researchers [15] have previously shown that anesthesia and surgical trauma significantly impairs functional characteristics of the small intestine. The results of chronic experiments on nonanesthetized animals [11,14,16] have shown that values of the maximal rate of the secondary active glucose transport under physiological conditions are significantly higher than the former estimates, based on the acute experiments in vivo.…”

Absorption of Glycine in the Small Intestine of Rats Under Physiological Condition

“…It means that surgery leads to increasing of the muscle tissue (possibly because of the partial replacing with the connective tissue as a result of operation). It comports to the data obtained by Ugolev et al [20,21]. …”

The active and passive components of glucose absorption in rat jejunum under low and high perfusion stress

“…The inference that both carrier‐mediated processes (SGLT1 and GLUT2) are diminished by anaesthesia and surgery in rat is strongly supported by the experiments described in Table 2 of Ugolev et al (1986). These workers measured rates of glucose absorption in chronic, unanaesthetised rats implanted with jejunal perfusion cannulae and compared them with rates measured in chronic, anaesthetised rats and acute, anaesthetised rats which had just undergone laparotomy (the same as in our experiments).…”

“…Thus in both our work and their work the ratio of the ‘passive’ to ‘active’ components was just over 2 in low stress perfusions. Table 7 of Ugolev et al (1986) also shows that phloridzin inhibits the rate of absorption of 110 m m glucose by 75 %; this value compares with the 71 % inhibition at 100 m m glucose (Kellett & Helliwell, 2000) and 79 % inhibition at 100 m m 3‐ O ‐methylglucose (Uhing & Kimura, 1995). Thus three different laboratories have obtained similar data in rather different ways over a 20 year time scale.…”

Kinetics and mechanisms of glucose absorption in the rat small intestine under physiological conditions

“…Parameters of the model with approximation in the form of a smooth surface are shown in Table 1. Their values were generally in the range of values that were determined previously in chronic experiments on rats [14,16]. The value of K t was between 0.2 -1.0 mM [2,5] and 2.0 -5.0 mmol/l [6,7,9].…”

“…Nevertheless, we [11,14,16] and other researchers [15] have previously shown that anesthesia and surgical trauma significantly impairs functional characteristics of the small intestine. The results of chronic experiments on nonanesthetized animals [11,14,16] have shown that values of the maximal rate of the secondary active glucose transport under physiological conditions are significantly higher than the former estimates, based on the acute experiments in vivo.…”