A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses

Abstract

Adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. The salt concentration of media is an important parameter controlling microbial adhesion. Salinity varies from 0.97% NaCl equivalents in the open eye to 0.89% in the closed eye state. In this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) NaCl on adhesion of ocular bacteria to soft contact lenses was investigated using a static adhesion assay. Pseudomonas aeruginosa was found to adhere to lenses in significantly greater amounts than Serratia marcescens, Flavobacterium meningosepticum, Stenotrophomonas maltophilia and Staphylococcus intermedius. Increasing NaCl from 0.8% to 1.0% (w/v) increased adhesion of all bacteria tested. This adhesion was strong since the organisms could not be removed by washing in low ionic buffer. Adhesion of these organisms did not correlate with their cell surface properties as determined by bacterial adhesion to hydrocarbons (BATH) and retention on sepharose columns.

Spoliation of fluoroperm rigid gas permeable contact lens by sodium chloride: A positron annihilation study

“…So these localized spots of drying may result in hydrophobic sites that attract lipids, hence leading to spoliation of the lens. This rationalization is in conformity with the work of Cowell et al, 8 who observed an increase in adhesion with increase in sodium chloride concentration.…”

Spoliation of fluoroperm rigid gas permeable contact lens by sodium chloride: A positron annihilation study

“…So these localized spots of drying may result in hydrophobic sites that attract lipids, hence leading to spoliation of the lens. This rationalization is in conformity with the work of Cowell et al, 8 who observed an increase in adhesion with increase in sodium chloride concentration.…”

Applications of ophthalmic biomaterials embedded with fucoidan

“…This result agrees with reports that Staphylococcus epidermidis and P. aeruginosa more effectively attached to contact lens in the presence of only HEMA compared with when both HEMA and MAA were present [50][51][52]. Antibacterial activity depends on hydrogels' water contents and charges [53,54].…”

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

“…Opportunistic pathogens like S. maltophilia are frequently encountered in hospitalized patients implanted with indwelling medical devices like catheters, prosthetic heart valves, contact lenses, and so forth [25–27]. Both clinical and environmental isolates of S. maltophilia are capable of adhering to biotic and abiotic surfaces such as glass, plastic materials, polyvinyl chloride, and Teflon [28, 29]. This study is one of the first reports demonstrating the antibacterial and antibiofilm activities against S. maltophilia .…”

Spoliation of fluoroperm rigid gas permeable contact lens by sodium chloride: A positron annihilation study

“…So these localized spots of drying may result in hydrophobic sites that attract lipids, hence leading to spoliation of the lens. This rationalization is in conformity with the work of Cowell et al, 8 who observed an increase in adhesion with increase in sodium chloride concentration.…”

Applications of ophthalmic biomaterials embedded with fucoidan

“…This result agrees with reports that Staphylococcus epidermidis and P. aeruginosa more effectively attached to contact lens in the presence of only HEMA compared with when both HEMA and MAA were present [50][51][52]. Antibacterial activity depends on hydrogels' water contents and charges [53,54].…”

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

“…Opportunistic pathogens like S. maltophilia are frequently encountered in hospitalized patients implanted with indwelling medical devices like catheters, prosthetic heart valves, contact lenses, and so forth [25–27]. Both clinical and environmental isolates of S. maltophilia are capable of adhering to biotic and abiotic surfaces such as glass, plastic materials, polyvinyl chloride, and Teflon [28, 29]. This study is one of the first reports demonstrating the antibacterial and antibiofilm activities against S. maltophilia .…”

Spoliation of fluoroperm rigid gas permeable contact lens by sodium chloride: A positron annihilation study

“…So these localized spots of drying may result in hydrophobic sites that attract lipids, hence leading to spoliation of the lens. This rationalization is in conformity with the work of Cowell et al, 8 who observed an increase in adhesion with increase in sodium chloride concentration.…”

Applications of ophthalmic biomaterials embedded with fucoidan

“…This result agrees with reports that Staphylococcus epidermidis and P. aeruginosa more effectively attached to contact lens in the presence of only HEMA compared with when both HEMA and MAA were present [50][51][52]. Antibacterial activity depends on hydrogels' water contents and charges [53,54].…”

In VitroAntibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates ofStenotrophomonas maltophiliaincluding the Trimethoprim/Sulfamethoxazole Resistant Strain

“…Opportunistic pathogens like S. maltophilia are frequently encountered in hospitalized patients implanted with indwelling medical devices like catheters, prosthetic heart valves, contact lenses, and so forth [25–27]. Both clinical and environmental isolates of S. maltophilia are capable of adhering to biotic and abiotic surfaces such as glass, plastic materials, polyvinyl chloride, and Teflon [28, 29]. This study is one of the first reports demonstrating the antibacterial and antibiofilm activities against S. maltophilia .…”