A regulatory element affects the activity and chromatin structure of the chicken α-globin 3′ enhancer

García-González, Estela G.; Recillas‐Targa, Félix

doi:10.1016/j.bbagrm.2014.09.009

Cited by 4 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ChIP primers are listed in Supplementary Table 2. EMSA. Electrophoretic Mobilitiy Shift Assay (EMSA) was performed as previously described with minor modifications 70 . In particular, nuclear protein was extracted using~40 × 10 6 Drosophila S2R+ cells.…”

Section: Methodsmentioning

confidence: 99%

In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

et al. 2020

Self Cite

View full text Add to dashboard Cite

Chromosomes are organized into high-frequency chromatin interaction domains called topologically associating domains (TADs), which are separated from each other by domain boundaries. The molecular mechanisms responsible for TAD formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by architectural proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as defects in transcription. Together, our results provide compelling evidence of the contribution of discrete genetic sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in the regulation of gene expression in Drosophila.

show abstract

Section: Methodsmentioning

confidence: 99%

In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Chromatin remodeling effects on enhancer activity

García-González

Escamilla-Del-Arenal

Arzate-Mejía

et al. 2016

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

During organism development, a diversity of cell types emerges with disparate, yet stable profiles of gene expression with distinctive cellular functions. In addition to gene promoters, the genome contains enhancer regulatory sequences, which are implicated in cellular specialization by facilitating cell-type and tissue-specific gene expression. Enhancers are DNA binding elements characterized by highly sophisticated and various mechanisms of action allowing for the specific interaction of general and tissue-specific transcription factors (TFs). However, eukaryotic organisms package their genetic material into chromatin, generating a physical barrier for TFs to interact with their cognate sequences. The ability of TFs to bind DNA regulatory elements is also modulated by changes in the chromatin structure, including histone modifications, histone variants, ATP-dependent chromatin remodeling, and the methylation status of DNA. Furthermore, it has recently been revealed that enhancer sequences are also transcribed into a set of enhancer RNAs with regulatory potential. These interdependent processes act in the context of a complex network of chromatin interactions, which together contributes to a renewed vision of how gene activation is coordinated in a cell-type-dependent manner. In this review, we describe the interplay between genetic and epigenetic aspects associated with enhancers and discuss their possible roles on enhancer function.

show abstract

In situdissection of domain boundaries affect genome topology and gene transcription inDrosophila

Arzate-Mejía

Cerecedo-Castillo

Guerrero

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

26The molecular mechanisms responsible for Topologically Associated Domains (TADs) 27 formation are not yet fully understood. In Drosophila, it has been proposed that 28 transcription is fundamental for TAD organization while the participation of genetic 29 sequences bound by Architectural Proteins (APs) remains controversial. Here, we 30 investigate the contribution of domain boundaries to TAD organization and the regulation 31 of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain 32 boundaries results in TAD fusion and long-range topological defects that are accompanied 33 by loss of APs and RNA Pol II chromatin binding as well as defects in transcription. 34 Together, our results provide compelling evidence on the contribution of discrete genetic 35 sequences bound by APs and RNA Pol II in the partition of the genome into TADs and in 36 the regulation of gene expression in Drosophila. 37 38

show abstract

A regulatory element affects the activity and chromatin structure of the chicken α-globin 3′ enhancer

Cited by 4 publications

References 36 publications

In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila

Chromatin remodeling effects on enhancer activity

In situdissection of domain boundaries affect genome topology and gene transcription inDrosophila

Contact Info

Product

Resources

About