A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus

Batten, Carrie; Banyard, Ashley C.; King, Donald P.; Henstock, Mark; Edwards, L.; Sanders, Anna; Buczkowski, Hubert; Oura, Chris C.L.; Barrett, Tom

doi:10.1016/j.jviromet.2010.11.022

Cited by 96 publications

(108 citation statements)

References 20 publications

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“…For the antigen detection on challenged animals, we used real time qPCR as described by Batten [11] for PPR and by Bowden [12] for SGP. DNA extraction was performed using isolate genomic DNA/RNA Mini kit (Bioline ® BIO-52066 & BIO-52075) and amplification done with the Kit superscript Tm III Platinum R one step qRt-PCR system ® (Cat.…”

Section: Materiel and Methodsmentioning

confidence: 99%

Field Application of a Combined Vaccine against Peste Des Petits Ruminants and Sheep Pox

Fakri¹,

Ghzal²,

Daouam³

et al. 2015

J Vaccines Vaccin

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A combined vaccine against peste des petits ruminants (PPR) and sheep/goat pox (SGP), based on Nigeria 75 PPR strain and sheep pox Romania strain, was developed and applied in the field. Safety and efficacy were evaluated on goats and sheep in comparison with monovalent PPR and SGP vaccines. Goats were challenged by PPR virulent strain and sheep by SP virulent strain. The result showsthat the combined PPR/SGP vaccine confers a good protection against both PPR and SGP infection with no significant difference with monovalent vaccines. The combined vaccine was used in Morocco and other African countries on small ruminant flocks with good safety and satisfactory sero-conversion detected against both PPR and SGP viruses, as soon as 14 days post vaccination. It's the first time where a combined vaccine was applied in large vaccination campaigns to prevent in one shot the two diseases.

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Section: Materiel and Methodsmentioning

confidence: 99%

Field Application of a Combined Vaccine against Peste Des Petits Ruminants and Sheep Pox

Fakri¹,

Ghzal²,

Daouam³

et al. 2015

J Vaccines Vaccin

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“…Peste des petits ruminants virus (PPRV) is a highly contagious, acute, febrile illness causing major economic losses small ruminant production and threatening agricultural balance in developing countries, including those in Africa, the Arabian Peninsula, the Middle East and Southeast Asia (Kwiatek et al 2010; Batten et al 2011). PPRV affects two small ruminants, namely sheep and goats, as a main host, but a large variety of ruminant species can also be susceptible to the infection (Albayrak and Gur 2010).…”

Section: Introductionmentioning

confidence: 99%

“…PPRV has a close antigenic relationship with rinderpest virus (RPV) in cattle, canine distemper virus (CDV) in dogs and phocine distemper virus (PDV) in seals (Kwiatek et al 2010). Mortality and morbidity is very high in naive animal populations, sometimes reaching rates of 90-100% (Albayrak and Gur 2010; Batten et al 2011;Gurcay et al 2013). PPRV infection is characterized by increased body temperature, anorexia, necrotic stomatitis, gastrointestinal symptoms like diarrhea, and inflammation of the respiratory tract (nasal and ocular discharges) (Ozkul et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…protein (M), fusion protein (F), haemagglutinin protein (H), large polymerase protein (L) and two nonstructural accessory proteins (C and L), which are produced by the RNA editing mechanisms of the P gene (Batten et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…A variety of diagnostic laboratory methods are used for confirming the presence of PPRV. For example, serologic detection methods, such as ELISA for PPRV, can be used to obtain antiviral antibodies, although this assay is laborious and time-consuming (Grant et al 2009;Batten et al 2011). Cell culture is also labor intensive and troublesome, rendering it unsuitable for routine diagnostic purposes.…”

Section: Introductionmentioning

confidence: 99%

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Development of real-time reverse transcriptase-polymerase chain reaction (rt RT- PCR) targeting four genes of peste des petits ruminants virus

ÖZKUL¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, one-step real-time quantitative reverse transcription PCR (qRT-PCR) was developed for the first time and evaluated for detection of peste des petits ruminants virus (PPRV) in field samples obtained from distinct geographical areas of Turkey. Primers and probes targeting four PPRV genes (namely L, M, N and P) were designed based on full genome sequence (AJ849636) of the local isolate (Tu00). The detection limits of the assays were found to be 7 copies/µL RNA for L, 6 copies/µL RNA for P, 10 copies/µL RNA for M and 700 copies/µL RNA for N, respectively. Besides, the detection ratio for PPRV in 45 field samples was 100% (45) for L, 88.9% (40) for M, 77.8% (35) for P and 22.3% (10) for N, respectively. In conclution, onestep real-time quantitative reverse transcription PCR (qRT-PCR) assay reported here for L gene design provides rapid, specific and sensitive detection of PPRV in tissue samples obtained from field cases.Key words: Peste des petits ruminants, Real-time qRT-PCR, virus detection. Küçük ruminant vebasının dört genini hedefleyen gerçek zamanlı reverz -transkriptaz polimeraz zincir reaksiyonu (rtRT -PZR)' nun geliştirilmesiÖzet: Bu çalışmada, tek basamaklı gerçek zamanlı kantitatif reverz transkripsiyon polimerize zincir reaksiyonu tekniği (qRT-PCR) geliştirildi ve Türkiye'nin farklı coğrafik alanlarından elde edilen saha örneklerinde küçük ruminant vebası virusunun (PPRV) tespiti için karşılaştırıldı. Yerel PPRV (Tu00)' nün dört genini (L, M, N ve P) hedefleyen primer ve problar ifade edilen virusun tüm genom dizini (AJ849636) temel alınarak tasarlandı. Çalışma kapsamında gerçekleştirilen tasarımlar ile her testin tespit sınırları, sırasıyla L geni için 7 kopya/µL RNA; P geni için 6 kopya/µL RNA; M geni için 10 kopya/µL RNA ve N geni için 700 kopya/µL RNA olarak bulundu. Bunun yanında, 45 saha örneği kullanılarak yapılan tanısal değerlendirmelerde ise virus genomunun tespit oranları sırasıyla L geni için %100 (45); M geni için %88.9 (40); P geni için %77.8 (35) ve N geni için %22.3 (10) olarak bulundu. Sonuç olarak, L geni dizaynı için bildirilen tek basamaklı gerçek zamanlı kantitatif reverz transkripsiyon polimerize zincir reaksiyonu deneyi (qRT-PCR) saha vakalarından elde edilen doku örneklerinde hızlı, spesifik ve hassas PPRV tespitini saplamıştır. Anahtar sözcükler: Küçük ruminant vebası, Gerçek zamanlı RT-PZR, virus tespiti.

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Peste des Petits Ruminants

Diallo¹,

Bataillé²,

Lancelot³

et al. 2019

Transboundary Animal Diseases in Sahelian Africa and Connected Regions

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A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus

Cited by 96 publications

References 20 publications

Field Application of a Combined Vaccine against Peste Des Petits Ruminants and Sheep Pox

Field Application of a Combined Vaccine against Peste Des Petits Ruminants and Sheep Pox

Development of real-time reverse transcriptase-polymerase chain reaction (rt RT- PCR) targeting four genes of peste des petits ruminants virus

Peste des Petits Ruminants

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