Gemella haemolysans is an uncommon but described cause of invasive disease in humans. We report a case of meningitis due to G. haemolysans that did not grow in cerebrospinal fluid culture, demonstrating a potential role for direct 16S rRNA gene PCR and sequencing in culture-negative cerebrospinal fluid when bacterial meningitis is suspected.
Case reportA 71-year-old man with hypertension, chronic obstructive pulmonary disease, pre-diabetes and chronic alcohol use presented from home to the Emergency Department with decreased responsiveness, nausea, vomiting, diarrhoea and subjective fever without headache. On presentation, his temperature was 97.8 u F, his heart rate was 150 min 21 and his blood pressure was 160/94 mmHg. He subsequently suffered a grand-mal seizure necessitating intubation and initiation of intravenous lorazepam. Post-ictal laboratory examination was noteworthy for a serum bicarbonate of 17.4 mmol l
21, lactic acid of 12 mmol l 21 and a white blood cell count of 20 700 ml 21 with 24 % bands. A computed tomography scan of the head was negative for acute stroke and he was admitted to the medical intensive care unit. In the Emergency Department, he had received ceftriaxone, vancomycin and clindamycin. During intensive care unit admission, the acid-base disturbances resolved, as did his leukocytosis. Electroencephalography did not demonstrate seizure activity. He was extubated on the second day of intensive care hospitalization and was alert and oriented but intermittently agitated and not at his baseline mental status off sedation. Lumbar puncture was performed the following day, which revealed an opening pressure of 44 cmH 2