A rapid method for the isolation of kidney brush border membranes

Malathi, P.; Preiser, H.; Fairclough, P. D.; Mallett, P.; Crane, Robert K.

doi:10.1016/0005-2736(79)90023-3

Cited by 186 publications

(57 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Brush border membranes (BBMs) from rat kidney and jejunum were isolated by a calcium precipitation method [15]. The final BBM pellet was suspended in 0.01 M phosphate buffer/0.15M NaCI, pH 7.4, and stored as 50 ,ul aliquots (approx.…”

Section: Methodsmentioning

confidence: 99%

Oligomeric structure of a renal cystine transporter: implications in cystinuria

Tate

1995

FEBS Letters

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Oligomeric structure of a renal cystine transporter: implications in cystinuria

Tate

1995

FEBS Letters

View full text Add to dashboard Cite

“…The brush border membrane vesicles were prepared essentially as previously described with a little modification (18). Transport studies were performed by rapid filtration technique at 25°C.…”

Section: Transport Studies In Renal Brush Border Membrane Vesiclesmentioning

confidence: 99%

Gene Knockout and Metabolome Analysis of Carnitine/Organic Cation Transporter OCTN1

et al. 2010

View full text Add to dashboard Cite

Purpose: Solute carrier, OCTN1 (SLC22A4) is an orphan transporter, the physiologically important substrate of which is still unidentified. The aim of the present study was to examine physiological roles of OCTN1. Methods:We first constructed octn1 gene knockout (octn1 -/-) mice. Metabolome analysis was then performed to identify substrates in vivo. The possible association of the substrate identified with diseased conditions was further examined. Results:The metabolome analysis of blood and several organs indicated complete deficiency of a naturally occurring potent antioxidant ergothioneine in octn1 -/-mice among 112 metabolites examined.Pharmacokinetic analyses after oral administration revealed the highest distribution to small intestines and extensive renal reabsorption of [ 3 H]ergothioneine, both of which were much reduced in octn1 -/-mice.The octn1 -/-mice exhibited greater susceptibility to intestinal inflammation under the ischemia and reperfusion model. The blood ergothioneine concentration was also much reduced in Japanese patients with Crohn's disease, compared with healthy volunteers and patients with another inflammatory bowel disease, ulcerative colitis. 4Conclusions: These results indicate that OCTN1 plays a pivotal role for maintenance of systemic and intestinal exposure of ergothioneine which could be important for protective effect against intestinal tissue injuries, providing a possible diagnostic tool to distinguish the inflammatory bowel diseases.

show abstract

“…Semeriva, personal communication). By contrast, when the bruh border membrane is purified according to [6] or [7], its active transport function is maintained, due to the fact that the overall procedure is accomplished in <3 h. However, from a chemical point of view and due to its very high level of purification the rat brush border membrane (this paper) appears well suited to perform lipid and protein analyses.…”

Section: Resultsmentioning

confidence: 97%