A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook &amp; Thoms. based on enhanced axillary branching

Abraham, Jyothi; Cheruvathur, Meena K.; Mani, Bince; Thomas, T. Dennis

doi:10.1016/j.indcrop.2009.09.011

Cited by 24 publications

(7 citation statements)

References 23 publications

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“…1 B). Repeated transfer of the original explants was suggested as an efficient technique for rejuvenation and reinvigoration of in vitro cultures ( Sanchez et al 1997 ), which was further supported by subsequent reports on different plants ( Abraham et al 2010 ; Ram and Shekhawat 2011 ; Phulwaria et al . 2012 b ).…”

Section: Resultsmentioning

confidence: 74%

A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

et al. 2012

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Nodal segments, obtained from 12 years-old mature plant, were used as explants for in vitro propagation of Celastrus paniculatus, an important medicinal plant of India. Shoot multiplication was achieved by repeated transfer of mother explants and subculturing of in vitro produced shoot clumps on MS medium supplemented with various concentrations of BAP alone or in combination with auxin (IAA or NAA). In vitro raised shoots were rooted under ex vitro condition. Genetic fidelity of the regenerated plants was assessed using random amplified polymorphic DNA (RAPD).

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Section: Resultsmentioning

confidence: 74%

A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

et al. 2012

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“…Thus, alternative propagation methods especially in vitro techniques are necessary for the rapid multiplication and conservation of this plant. Plant tissue culture technique has been recognized as an alternative method of plant propagation and production of large number of uniform, elite clones in a limited period of time (Abraham et al 2010;Kumar and Thomas 2012). This method is also useful for the production, characterization and use of valuable secondary metabolites for pharmaceutical use (Murthy et al 2014;Cheruvathur and Thomas 2014).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Abraham

Thomas

2015

Physiol Mol Biol Plants

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An efficient callus induction and plant regeneration system has been standardized for an ethnomedicinal plant, Elephantopus scaber Linn. Two explants i. e. seeds and leaf segments were used for callus induction. Murashige and Skoog (MS) medium supplemented with 5.0 μM 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0.5 μM kinetin (Kn) gave the optimum frequency (89 %) of callus induction from seed explant. The results showed that the highest response in terms of percent callus regenerating (91 %) and number of shoots (56) per culture was recorded on MS medium supplemented with 6.0 μM N6-benzylaminopurine (BA) and 1.5 μM α naphthalene acetic acid (NAA). The best rooting of regenerated shoots was obtained on half strength MS medium supplemented with 6.0 μM indole-3- butyric acid (IBA). On this medium, 100 % of the shoots produced roots with a mean number of 3.2 roots per shoot. The positive role of vesicular arbuscular mycorrhizae (VAM) along with potting mix has been well established in the present study. Of the various potting mix employed for plant acclimatization, the highest response of 100 % plant survival was noticed when autoclaved garden soil, sand (2:1) and VAM was utilized as potting mix. Inter-simple sequence repeats (ISSR) were used to establish the clonal fidelity of regenerated plantlets and the banding profiles from callus derived plants were monomorphic and similar to those of mother plant, thus ascertaining the true-to-type nature of these plants.

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“…The superiority of BAP over Kin for shoot initiation has also been reported in Cyclea peltata [3] and Morinda citrifolia [9] etc. There were differences among the treatments for mean number of shoots/explant.…”

Section: Resultsmentioning

confidence: 56%

Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’

et al. 2021

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Optimization of in vitro culture conditions of grapevine phylloxera-resistant rootstock cultivar ‘Ruggeri-140’(Vitisberlandieri x Vitisrupestris) was carried out. Among the different sterilization treatments, maximum aseptic cultures were obtained for both explants apical tips and nodal segments when treated with Ca(ClO)2 at concentration of 1.5 % for 10 minutes plus 70 % ethanol for 30 s (T7). The maximum shoot proliferation was observed both in apical and nodal meristems cultured on MS medium supplemented with 1.0 mg/l BAP. MS/2 medium containing 1.0 mg/l indole-3-butric acid (IBA) gave the highest rooting percentage (100%) with the highest mean number and length of roots. The ex vitro survival of rooted micro shoots was 75.0%.

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A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook & Thoms. based on enhanced axillary branching

Cited by 24 publications

References 23 publications

A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

A genetically stable rooting protocol for propagating a threatened medicinal plant--Celastrus paniculatus

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’

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