A rapid, eco-friendly, and reliable microplate method for determination of Cr(VI)

Coreño-Alonso, Alejandro; Jiménez, Gerardo; López-Martínez, Leticia; López, G.E. Reyna; Aguilar, Francisco Javier Acevedo

doi:10.3906/kim-1609-53

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…After 24 h, K 2 Cr 2 O 7 was added to each of the cultures ([Cr(VI)] 0 = 70 μM) and Fe(II) concentrations were analyzed. Cr(VI) concentration in the supernatant was analyzed using the DPC assay 63 . The DPC assay was performed by mixing 15 μL of sample with 235 μL of DPC solution in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Microbial reduction of metal-organic frameworks enables synergistic chromium removal

Springthorpe¹,

Dundas²,

Keitz³

2019

Nat Commun

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Redox interactions between electroactive bacteria and inorganic materials underpin many emerging technologies, but commonly used materials (e.g., metal oxides) suffer from limited tunability and can be challenging to characterize. In contrast, metal-organic frameworks exhibit well-defined structures, large surface areas, and extensive chemical tunability, but their utility as microbial substrates has not been examined. Here, we report that metal-organic frameworks can support the growth of the metal-respiring bacterium Shewanella oneidensis, specifically through the reduction of Fe(III). In a practical application, we show that cultures containing S. oneidensis and reduced metal-organic frameworks can remediate lethal concentrations of Cr(VI) over multiple cycles, and that pollutant removal exceeds the performance of either component in isolation or bio-reduced iron oxides. Our results demonstrate that frameworks can serve as growth substrates and suggest that they may offer an alternative to metal oxides in applications seeking to combine the advantages of bacterial metabolism and synthetic materials.

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Section: Methodsmentioning

confidence: 99%

Microbial reduction of metal-organic frameworks enables synergistic chromium removal

Springthorpe¹,

Dundas²,

Keitz³

2019

Nat Commun

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“…Samples that were collected from solution at regular time intervals were immediately centrifuged (×10000 g , 10 min) to stop further reaction. The supernatant was collected and the Cr concentration monitored using the 1,5-diphenylcarbazide assay (DPC) reaction [ 33 ], against a standard calibration curve for known Cr(VI) concentrations measured at a wavelength λ =540 nm.…”

Section: Methodsmentioning

confidence: 99%

Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

et al. 2021

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Biomineralization is a ubiquitous process in organisms to produce biominerals, and a wide range of metallic nanoscale minerals can be produced as a consequence of the interactions of micro-organisms with metals and minerals. Copper-bearing nanoparticles produced by biomineralization mechanisms have a variety of applications due to their remarkable catalytic efficiency, antibacterial properties and low production cost. In this study, we demonstrate the biotechnological potential of copper carbonate nanoparticles (CuNPs) synthesized using a carbonate-enriched biomass-free ureolytic fungal spent culture supernatant. The efficiency of the CuNPs in pollutant remediation was investigated using a dye (methyl red) and a toxic metal oxyanion, chromate Cr(VI). The biogenic CuNPs exhibited excellent catalytic properties in a Fenton-like reaction to degrade methyl red, and efficiently removed Cr(VI) from solution due to both adsorption and reduction of Cr(VI). X-ray photoelectron spectroscopy (XPS) identified the oxidation of reducing Cu species of the CuNPs during the reaction with Cr(VI). This work shows that urease-positive fungi can play an important role not only in the biorecovery of metals through the production of insoluble nanoscale carbonates, but also provides novel and simple strategies for the preparation of sustainable nanomineral products with catalytic properties applicable to the bioremediation of organic and metallic pollutants, solely and in mixtures.

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“…After 24 h, K 2 Cr 2 O 7 was added to each of the cultures ([Cr(VI)]0=70 μM) and Fe(II) concentrations were analyzed. Cr(VI) concentration in the supernatant was analyzed using the DPC assay (50) . The DPC assay was performed by mixing 15 μL of sample with 235 μL of DPC solution in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Microbial Reduction of Metal-Organic Frameworks Enables Synergistic Chromium Removal

Springthorpe

Dundas

Keitz

2018

Preprint

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9Microbial interactions with redox-active materials are ubiquitous in geochemical cycling and 10 bioelectrochemical devices, but the biotic-abiotic interface has proven challenging to study due to 11 the structural complexity of mineral substrates. In contrast, metal-organic frameworks are a class Cr(VI) adsorption capacity, demonstrating that the framework confers protection to the bacteria 22 and that no regenerative step is needed for continued bioremediation. In sum, our results show 23 that the study of microbial-material interactions can be extended to metal-organic frameworks and 24 suggest that these materials may offer a promising alternative to metal oxides in applications 25 seeking to combine the advantages of bacterial metabolism and synthetic materials.

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A rapid, eco-friendly, and reliable microplate method for determination of Cr(VI)

Cited by 5 publications

References 23 publications

Microbial reduction of metal-organic frameworks enables synergistic chromium removal

Microbial reduction of metal-organic frameworks enables synergistic chromium removal

Application of fungal copper carbonate nanoparticles as environmental catalysts: organic dye degradation and chromate removal

Microbial Reduction of Metal-Organic Frameworks Enables Synergistic Chromium Removal

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