A rapid and simple method to isolate pure alpha-cellulose

Abstract

A protocol is described to isolate small quantities of highly purified cellulose for isotopic analysis of 10–100 mg samples of secondary (Pinus sylvestris L.) and primary (Rubus idaeus L.) plant cell wall material. Elemental analysis of 350 cellulose samples isolated from pine wood samples estimated the relative carbon content to be ca. 43.7% ± 1.2%. This value indicates that the cellulose quality is high and that the protocol is highly reproducible. High‐performance anion exchange chromatography with pulsed amperometric detection of hydrolysis products quantified the purity of the cellulose as ca. 99% for wood cellulose and primary cell wall cellulose. DRIFT spectroscopy corroborated this purity and found no evidence of cellulose degradation. Carbon isotopic composition of the purified cellulose using mass spectrometry was measured with an accuracy of 0.11‰ (standard deviation). The method is rapid (56 samples may be routinely processed within 8 h) and requires only standard laboratory equipment and chemicals. Copyright © 2000 John Wiley & Sons, Ltd.

Comparative Study of Crude and Purified Cellulose from Wheat Straw

“…However, it should be noted that the spectra provides evidence of slight acetylation by showing the presence of the three acetyl ester bands at 1745 (CdO ester), 1387 (-C-CH 3 ), and -C-Ostretching band at 1261 cm -1 (44), indicating that acetylation occurred during the final stage treatment with 80% acetic acid-70% nitric acid under the conditions used. The current findings were not consistent with the conclusions obtained by Brendel et al (19). The authors presumed that these acetyl esters would be derived from arabino-4-O-methylglucoronoxylans reported to be predominant in pine wood.…”

Determination of Bovine Serum Albumin Adsorption Capacity of Newly Obtained Cellulose extracted from Glycyrrhiza glabra (Licorice)

“…The cellulose content of G. glabra roots were estimated as 48.51%. Yield of pure cellulose from 7.77 g of G. glabra sample was recorded as 4.0 g. This value was found close to the values reported in previous cellulose isolation literature; 29–30% for hard wood, 32.8% for raw rice husk, 33.98% for capimmombaca ( Panicum maximum ), 49.43% for blue agave bagasse fibers, and 21.4% to 44.7% for pumpkins ( Cucurbitas P .) .…”

Consequences of a Rapid Cellulose Extraction Technique for Oxygen Isotope and Radiocarbon Analyses

“…In agreement with the paired t -test reported above, an analysis of variance (ANOVA) on unprocessed, Brendel, and Leavitt-Danzer extracted SAC also shows no significant difference in the measured mean at the nominal instrument precision (0.3‰). These findings are consistent with previously reported results 18 for a small set of δ 18 O samples, as well as for δ 13 C as reported by Brendel et al, but differs from the conclusions of Gaudinski et al . This discrepancy may be the result of comparing different techniques across tissue types, (leaves, wood, roots), instead of considering xylem wood separately.…”