A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line

Hoek, Angela H.A.M. van; Jonge, R. de; Overbeek, Wendy M. van; Bouw, El; Pielaat, Annemarie; Smid, Joost; Malorny, Burkhard; Junker, Ernst; Löfström, Charlotta; Pedersen, Karl; Aarts, H.J.M.; Heres, L.

doi:10.1016/j.ijfoodmicro.2011.10.013

Cited by 66 publications

(63 citation statements)

References 22 publications

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“…In addition, identification of specific serotypes (Table 3) in ABF carcass and processing plant environmental samples, which were not detected at the farm level, suggests the role of the slaughter environment as a source for cross-contamination. Identification of serotypes, including S. Agona, S. Braenderup, S. Derby, S. Inverness, S. Muenchen, and S. Newport, which were not found in farm and slaughter environments on ABF carcass (Table 3) is attributed to the slaughter robots, including the carcass splitter and other instruments used for processing the carcass as a possible source of contamination during the production chain as described previously (39). We observed some common serotypes in the ABF and conventional production systems, including S. Anatum, S. Infantis, and S. Typhimurium.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Keelara

Scott

Morrow

et al. 2013

Appl Environ Microbiol

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The aim of this longitudinal study was to determine and compare the prevalences and genotypic profiles of antimicrobial-resistant (AR) Salmonella isolates from pigs reared in antimicrobial-free (ABF) and conventional production systems at farm, at slaughter, and in their environment. We collected 2,889 pig fecal and 2,122 environmental (feed, water, soil, lagoon, truck, and floor swabs) samples from 10 conventional and eight ABF longitudinal cohorts at different stages of production (farrowing, nursery, finishing) and slaughter (postevisceration, postchill, and mesenteric lymph nodes [MLN]). In addition, we collected 1,363 carcass swabs and 205 lairage and truck samples at slaughter. A total of 1,090 Salmonella isolates were recovered from the samples; these were isolated with a significantly higher prevalence in conventionally reared pigs (4.0%; n ‫؍‬ 66) and their environment (11.7%; n ‫؍‬ 156) than in ABF pigs (0.2%; n ‫؍‬ 2) and their environment (0.6%; n ‫؍‬ 5) (P < 0.001). Salmonella was isolated from all stages at slaughter, including the postchill step, in the two production systems. Salmonella prevalence was significantly higher in MLN extracted from conventional carcasses than those extracted from ABF carcasses (P < 0.001). We identified a total of 24 different serotypes, with Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Anatum, Salmonella enterica serovar Infantis, and Salmonella enterica serovar Derby being predominant. The highest frequencies of antimicrobial resistance (AR) were exhibited to tetracycline (71%), sulfisoxazole (42%), and streptomycin (17%). Multidrug resistance (resistance to >3 antimicrobials; MDR) was detected in 27% (n ‫؍‬ 254) of the Salmonella isolates from the conventional system. Our study reports a low prevalence of Salmonella in both production systems in pigs on farms, while a higher prevalence was detected among the carcasses at slaughter. The dynamics of Salmonella prevalence in pigs and carcasses were reciprocated in the farm and slaughter environment, clearly indicating an exchange of this pathogen between the pigs and their surroundings. Furthermore, the phenotypic and genotypic fingerprint profile results underscore the potential role played by environmental factors in dissemination of AR Salmonella to pigs.

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Section: Discussionmentioning

confidence: 99%

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Keelara

Scott

Morrow

et al. 2013

Appl Environ Microbiol

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“…In industrialized countries, meat samples, if contaminated, often harbor very low numbers of Salmonella (11,12); therefore, an enrichment step is an inevitable part of molecular detection methods. Ideally, this enrichment should maximize the number of Salmonella organisms while limiting the background flora.…”

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confidence: 99%

“…Omitting the time-consuming enrich-ment step would therefore be optimal, and several direct detection methods have also been published (9,(18)(19)(20). However, most of these methods are complex, not high throughput, and have detection limits of Ͼ50 CFU/25 g, i.e., above the reported levels of Salmonella in meat (12). Moreover, the legislative demand of absence of 1 CFU/25 g (21) cannot currently be met by the available direct detection technologies, making some sort of short-culture enrichment step necessary.…”

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confidence: 99%

Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method

Fachmann

Löfström

Hoorfar

et al. 2017

Appl Environ Microbiol

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Salmonella is recognized as one of the most important foodborne bacteria and has wide health and socioeconomic impacts worldwide. Fresh pork meat is one of the main sources of Salmonella, and efficient and fast methods for detection are therefore necessary. Current methods for Salmonella detection in fresh meat usually include Ͼ16 h of culture enrichment, in a few cases Ͻ12 h, thus requiring at least two working shifts. Here, we report a rapid (Ͻ5 h) and high-throughput method for screening of Salmonella in samples from fresh pork meat, consisting of a 3-h enrichment in standard buffered peptone water and a real-time PCR-compatible sample preparation method based on filtration, centrifugation, and enzymatic digestion, followed by fast-cycling real-time PCR detection. The method was validated in an unpaired comparative study against the Nordic Committee on Food Analysis (NMKL) reference culture method 187. Pork meat samples (n ϭ 140) were either artificially contaminated with Salmonella at 0, 1 to 10, or 10 to 100 CFU/25 g of meat or naturally contaminated. Cohen's kappa for the degree of agreement between the rapid method and the reference was 0.64, and the relative accuracy, sensitivity, and specificity for the rapid method were 81.4, 95.1, and 97.9%, respectively. The 50% limit of detections (LOD 50 s) were 8.8 CFU/25 g for the rapid method and 7.7 CFU/25 g for the reference method. Implementation of this method will enable faster release of Salmonella low-risk meat, providing savings for meat producers, and it will help contribute to improved food safety.IMPORTANCE While the cost of analysis and hands-on time of the presented rapid method were comparable to those of reference culture methods, the fast product release by this method can provide the meat industry with a competitive advantage. Not only will the abattoirs save costs for work hours and cold storage, but consumers and retailers will also benefit from fresher meat with a longer shelf life. Furthermore, the presented sample preparation might be adjusted for application in the detection of other pathogenic bacteria in different sample types.KEYWORDS Salmonella, food safety, foodborne pathogens, fresh meat, rapid tests, real-time PCR, sample preparation, validation S almonella is a global foodborne pathogen responsible for approximately 30% of foodborne outbreaks in the United States and 23% in the European Union (1, 2). Pork meat remains an important source of human salmonellosis, which is why the absence of Salmonella is confirmed at critical control points at abattoirs (2). However, the reference culture-based methods require several days to obtain a test result (3, 4), thereby postponing the market release of fresh meat.

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“…Numerous studies have identified S. Rissen in slaughtered pigs in Portugal [4] and within pig fattening units and from slaughter-age pigs and Spain [5] [6] [7] [8] [9]. There have been further reports of S. Rissen in pig abattoir studies in Italy [10], Belgium [11], the Netherlands [12], Portugal [13] as well as in breeding herds in the UK [14] and in finishing pigs in Northern Ireland [15], thus demonstrating its' increase in European pig herds.…”

Section: Introductionmentioning

confidence: 99%

Detection of <i>Salmonella enterica</i> Serovar Rissen in Slaughter Pigs in Northern Ireland

Egan¹,

Naughton²,

Dooley³

et al. 2017

AiM

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Salmonella enterica serovar Rissen has been recognised as a common serovar in humans and pigs around the world. This study investigated S. Rissen prevalence in pigs slaughtered in Northern Ireland additionally looking at antibiotic susceptibility profiles, genetic profiles and plasmid profiles to provide information on an emerging non-typhoid Salmonella serotype with the potential to cause disease in humans. S. Rissen were isolated on five separate sampling occasions from both the boning hall and slaughter line of a randomly selected single pig abattoir in Northern Ireland (NI). Following antibiotic susceptibility testing against 16 antibiotics, all S. Rissen isolates were identified as susceptible to 15 antibiotics but resistant to tetracycline (R-type: Te).Of the 29 S. Rissen, 27 were isolated from pigs originating in NI and two S.Rissen were isolated from pigs originating in the Republic of Ireland (RoI). The combined results of the PFGE and plasmid profiling analyses were capable of subdividing the S. Rissen isolates into three distinct groups. The data suggests that S. Rissen is an emerging serovar in Northern Ireland and continued surveillance of this serovar is warranted as it has the potential to cause disease in humans.

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A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line

Abstract: 26Pork contributes significantly to the public health disease burden caused by Salmonella

Cited by 66 publications

References 22 publications

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method

Detection of <i>Salmonella enterica</i> Serovar Rissen in Slaughter Pigs in Northern Ireland

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A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line

Abstract: 26Pork contributes significantly to the public health disease burden caused by Salmonella

Cited by 66 publications

References 22 publications

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Longitudinal Study of Distributions of Similar Antimicrobial-Resistant Salmonella Serovars in Pigs and Their Environment in Two Distinct Swine Production Systems

Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method

Detection of &lt;i&gt;Salmonella enterica&lt;/i&gt; Serovar Rissen in Slaughter Pigs in Northern Ireland

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Detection of <i>Salmonella enterica</i> Serovar Rissen in Slaughter Pigs in Northern Ireland