A quadruple wavelength IR sensor system for label-free tumour screening

Driesche, Sander van den; Witarski, Wojciech; Pastoreková, Silvia; Vellekoop, Michael J.

doi:10.1088/0957-0233/20/12/124015

Cited by 9 publications

(9 citation statements)

References 8 publications

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“…[15][16][17] To distinguish normal from malignant cells we propose a sensing principle that is based on a four-wavelength mid-infrared absorbance measurement, to derive the CH 2 -symmetric/CH 2 -antisymmetric stretch ratio. 18 The monitoring of CH 2 -stretches of phospholipid acyl enables to look at changes in molecular interactions and it inherently compensates for differences in the cell amount and measurement spot size. Instead of measuring the frequency shifts of either the CH 2 -symmetric or CH 2 -antisymmetric stretch caused by trans/ gauche conformation changes of the acyl chain, 19 the cell type discrimination method depends on the ratio change between the two CH 2 -stretches.…”

Section: Introductionmentioning

confidence: 99%

A label-free indicator for tumor cells based on the CH2-stretch ratio

Driesche

Witarski

Pastoreková

et al. 2011

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In this paper, we assess the potential of a label-free infrared absorbance based measurement method for determination of the CH(2)-symmetric to CH(2)-antisymmetric stretch ratio, to aid in the detection of the presence of cancer cells and to differentiate between various cancer cells. For this study a normal epithelial kidney cell line, two carcinoma epithelial kidney cell lines, an adult primary human melanocyte cell line, and three human melanoma cell lines were investigated. For the measurements we used a self-designed IR sensor which has the potential to be further developed in a point-of-care instrument. To investigate the mechanism influencing the CH(2)-stretch ratio of mammalian cell membranes, a normal epithelial kidney cell line was exposed to the plasma membrane bound cholesterol reducing agent methyl-β-cyclodextrin. This methodology yielded statistically significant CH(2)-stretch ratio differences between the individual cell lines, normal and tumorous, of both epithelial kidney and melanocyte origin. Measurement results of normal epithelial kidney cells exposed to methyl-β-cyclodextrin indicate that an increase in the CH(2)-stretch ratio arises when there is a decrease in, or redistribution of, the membrane stabilizing agent cholesterol. This study proves that the proposed cell type discrimination method, based on the CH(2)-symmetric to CH(2)-antisymmetric stretch ratio, allows the discrimination between normal and tumor cells. In addition, the method shows high potential for improvement of staging of suspicious tissues.

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Section: Introductionmentioning

confidence: 99%

A label-free indicator for tumor cells based on the CH2-stretch ratio

Driesche

Witarski

Pastoreková

et al. 2011

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“…In contrast, signal in infrared spectroscopy originates with the most intrinsic part of a sample—its molecular structure. Infrared measurements can thus be leveraged for automated tissue classification and cancer identification5678. Most significant is their ability to record the conformational changes of proteins that elucidate the molecular mechanisms of their function9101112.…”

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confidence: 99%

In-situ ultra-sensitive infrared absorption spectroscopy of biomolecule interactions in real time with plasmonic nanoantennas

2013

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Infrared absorption spectroscopy is a powerful biochemical analysis tool as it extracts detailed molecular structural information in a label-free fashion. Its molecular specificity renders the technique sensitive to the subtle conformational changes exhibited by proteins in response to a variety of stimuli. Yet, sensitivity limitations and the extremely strong absorption bands of liquid water severely limit infrared spectroscopy in performing kinetic measurements in biomolecules’ native, aqueous environments. Here we demonstrate a plasmonic chip-based technology that overcomes these challenges, enabling the in-situ monitoring of protein and nanoparticle interactions at high sensitivity in real time, even allowing the observation of minute volumes of water displacement during binding events. Our approach leverages the plasmonic enhancement of absorption bands in conjunction with a non-classical form of internal reflection. These features not only expand the reach of infrared spectroscopy to a new class of biological interactions but also additionally enable a unique chip-based technology.

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“…In the case of cancer, a reduction in the cholesterol concentration leads to a molecular disorder with a consequent increase of the CH 2 symmetric stretch vibration [48]. Our previous studies on melanoma cell lines showed an increase in the CH 2 stretch ratio when cholesterol is sequestered from the plasma membrane [43]. …”

Section: Discussionmentioning

confidence: 99%

“…Indeed, anomalies in the infrared absorption of methylene (CH 2 ) groups have been reported in the literature in the occurrence of cancer [41,42]. We realized an infrared quadruple sensor for the CH 2 stretch ratio determination of different melanoma cell lines [43,44]. Increased CH 2 stretch ratio values were observed for melanoma cells compared to the healthy reference.…”

Section: Introductionmentioning

confidence: 99%

An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies

Fioravanti

Brandhoff

Driesche

et al. 2016

Sensors

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An infrared (IR) absorbance sensor has been designed, realized and tested with the aim of detecting malignant melanomas in human skin biopsies. The sensor has been designed to obtain fast measurements (80 s) of a biopsy using a small light spot (0.5 mm in diameter, typically five to 10 times smaller than the biopsy size) to investigate different biopsy areas. The sensor has been equipped with a monochromator to record the whole IR spectrum in the 3330–3570 nm wavelength range (where methylene and methyl stretching vibrations occur) for a qualitative spectral investigation. From the collected spectra, the CH2 stretch ratio values (ratio of the absorption intensities of the symmetric to asymmetric CH2 stretching peaks) are determined and studied as a cancer indicator. Melanoma areas exhibit different spectral shapes and significantly higher CH2 stretch ratios when compared to healthy skin. The results of the infrared investigation are compared with standard histology. This study shows that the IR sensor is a promising supportive tool to improve the diagnosis of melanoma during histopathological analysis, decreasing the risk of misdiagnosis.

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A quadruple wavelength IR sensor system for label-free tumour screening

Cited by 9 publications

References 8 publications

A label-free indicator for tumor cells based on the CH2-stretch ratio

A label-free indicator for tumor cells based on the CH2-stretch ratio

In-situ ultra-sensitive infrared absorption spectroscopy of biomolecule interactions in real time with plasmonic nanoantennas

An Infrared Absorbance Sensor for the Detection of Melanoma in Skin Biopsies

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