A putative cell signal encoded by the folded gastrulation gene coordinates cell shape changes during Drosophila gastrulation

Costa, Michael A.; Wilson, Ellen T.; Wieschaus, Eric

doi:10.1016/0092-8674(94)90384-0

Cited by 294 publications

(304 citation statements)

References 46 publications

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“…Briefly, Toll signaling induces the expression of transcriptional regulators Twist (Twi) and Snail (Sna). Twi activates the expression of folded gastrulation (Fog) and T48 (Costa et al, 1994;Kolsch et al, 2007). Fog is a secreted factor that binds to an as-yet-unidentified receptor to activate the Ga protein, Concertina (Cta) at the apical surface of ventral cells.…”

Section: Developmental Dynamicsmentioning

confidence: 99%

“…This may either indicate that Sna is required for both phases of apical constriction, or that concerted apical constriction is dependent on the proper initiation of random apical constriction. twi mutant embryos have typical random apical constriction, but fail to develop concerted apical constrictions, thus random apical constriction phase appears to be Twiindependent while the concerted apical constriction phase requires Twi function (Costa et al, 1994;Ip et al, 1994;Dawes-Hoang et al, 2005;Martin et al, 2009;Pouille et al, 2009). A key downstream target of Twi is the secreted signaling protein, Fog.…”

Section: Critical Period For Rok Activity During Vffmentioning

confidence: 99%

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Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation

Krajcovic

Minden

2012

Developmental Dynamics

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Background: Drosophila ventral furrow formation (VFF), which is the first morphogenetic event during embryo development, serves as a model for epithelial sheet folding. VFF can be subdivided into five cell shape changes: apical membrane flattening, apicobasal nuclear migration, apicobasal cell shortening, random apical constriction, and concerted apical constriction. These processes are generally believed to be driven by Rho kinase (Rok) activation of myosin II to stimulate the constriction of the apical actomyosin network. To test the role of Rok and its downstream target myosin II in VFF, timed injections of the Rok inhibitor, Y-27632, were performed. Results: Embryos injected with Y-27632 before the concerted apical constriction phase of VFF were able to execute apicobasal nuclear migration and random apical constriction, but were unable to enter the concerted apical constriction phase. Embryos injected with Y-27632 during concerted apical constriction reverted to the transition point between random apical constriction and concerted apical constriction. Finally, embryos injected with Y-27632 upon the initiation of furrow ingression were able to complete VFF. Conclusions: Together these results suggest a critical period for Rok activity and presumably myosin II activation during the initiation of the concerted apical constriction phase of VFF.

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Section: Developmental Dynamicsmentioning

confidence: 99%

Section: Critical Period For Rok Activity During Vffmentioning

confidence: 99%

Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation

Krajcovic

Minden

2012

Developmental Dynamics

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“…Although there is a detailed understanding of the molecular mechanisms underlying developmental patterning (Costa et al, 1994;Leptin, 1999), little is known about the process by which genetic information is translated via mechanics into physical form. Historically, efforts to study morphogenesis have been limited by the theoretical and technical difficulties of measuring, modelling and perturbing relevant forces and material properties in living systems.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, these cells undergo a set of major changes in shape and topology, namely invagination on the ventral side and at the posterior end, which gives rise to the mesoderm and midgut (Leptin, 1995). The genetic control of the ventral invagination has been associated with the snail and twist genes that define the region involved and control the initiation of the signalling pathway Costa et al, 1994;Leptin, 1995). The kinematics of invagination is characterised by the apical flattening and subsequent apical constriction of the cells in the ventral area (Sweeton et al, 1991;Leptin and Grunewald, 1990;Kam et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

A 3D finite element model of ventral furrow invagination in the Drosophila melanogaster embryo

Conte

Muñoz²,

Miodownik

2008

Journal of the Mechanical Behavior of Biomedical Materials

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Ventral furrow formation is the first large-scale movement in the Drosophila cellular blastoderm which involves the co-ordinated shape change of cells, and as such is an ideal system to use as a proof of principle for simulation methods to study the mechanics of morphogenesis. We have developed a 3D finite element method model of ventral furrow formation by decomposing the total deformation into two parts: an imposed active deformation, and an elastic passive deformation superimposed onto the latter. The model imposes as boundary conditions (i) Vito, C., Muñoz, J.J., Miodownik M., A 3D finite element model of ventral furrow invagination in the Drosophila melanogaster embryo, Journal of the Mechanical Behavior of Biomedical Materials, Vol. 1, Issue 2, pp. 188-198, 2008 compared the model with a 2D model and shown that it exhibits a more robust invagination phenomenon. The 3D model has also revealed that invagination causes a yolk flow from the central region to the anterior and posterior ends of the embryo, causing an accordion-like global compression and expansion wave to move through the embryo. Such phenomenon cannot be described by 2D models.

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“…The first step in the EMT of Drosophila mesoderm is ventral furrow formation, which begins with an apical constriction and cell shape change similar to that seen in amphibian bottle cells. Coordination of apical constriction requires a secreted ligand, folded gastrulation (Fog; Costa et al 1994) acting upstream of concertina (Cta), which encodes a Ga protein (Parks & Wieschaus 1991;Morize et al 1998), through G-protein-coupled receptor that has not been identified. Downstream of concertina, the rho activator, DRhoGEF2, and Rho1 are essential for the accumulation of myosin II at the cell apices (Barrett et al 1997;Hacker & Perrimon 1998;Nikolaidou & Barrett 2004;Rogers et al 2004;Dawes-Hoang et al 2005).…”

Section: What Is the Molecular Mechanism Of Progressive Expression Ofmentioning

confidence: 99%

Dynamic determinations: patterning the cell behaviours that close the amphibian blastopore

Keller

Shook

2008

Phil. Trans. R. Soc. B

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We review the dynamic patterns of cell behaviours in the marginal zone of amphibians with a focus on how the progressive nature and the geometry of these behaviours drive blastopore closure. Mediolateral cell intercalation behaviour and epithelial-mesenchymal transition are used in different combinations in several species of amphibian to generate a conserved pattern of circumblastoporal hoop stresses. Although these cell behaviours are quite different and involve different germ layers and tissue organization, they are expressed in similar patterns. They are expressed progressively along presumptive lateral-medial and anterior-posterior axes of the body plan in highly ordered geometries of functional significance in the context of the biomechanics of blastopore closure, thereby accounting for the production of similar patterns of circumblastoporal forces. It is not the nature of the cell behaviour alone, but the context, the biomechanical connectivity and spatial and temporal pattern of its expression that determine specificity of morphogenic output during gastrulation and blastopore closure. Understanding the patterning of these dynamic features of cell behaviour is important and will require analysis of signalling at much greater spatial and temporal resolution than that has been typical in the analysis of patterning tissue differentiation.

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A putative cell signal encoded by the folded gastrulation gene coordinates cell shape changes during Drosophila gastrulation

Cited by 294 publications

References 46 publications

Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation

Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation

A 3D finite element model of ventral furrow invagination in the Drosophila melanogaster embryo

Dynamic determinations: patterning the cell behaviours that close the amphibian blastopore

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