A proteome-wide protein interaction map for Campylobacter jejuni

Abstract

Background: Data from large-scale protein interaction screens for humans and model eukaryotes have been invaluable for developing systems-level models of biological processes. Despite this value, only a limited amount of interaction data is available for prokaryotes. Here we report the systematic identification of protein interactions for the bacterium Campylobacter jejuni, a foodborne pathogen and a major cause of gastroenteritis worldwide.

Genome Reduction Promotes Increase in Protein Functional Complexity in Bacteria

“…We obtained information for all unique, binary PPIs in the genomes of M. tuberculosis, Synechocystis sp., Helicobacter pylori, Campylobacter jejuni, Treponema pallidum, Mycoplasma pneumonia, and Escherichia coli (Rain et al 2001;Parrish et al 2007;Sato et al 2007;Titz et al 2008;Kuhner et al 2009;Peregrin-Alvarez et al 2009;Wang et al 2010).…”

Preparation and Characterization of Bacterial Protein Complexes for Structural Analysis

“…Systematic large-scale studies of bacterial protein interaction networks at the level of whole proteomes have been initiated using the yeast 2-hybrid (Y2H) method as applied to a subset of the genome of Helicobacter pylori (Rain et al, 2001), Synechocystis sp. PCC6803 (Sato et al, 2007), and Campylobacter jejuni (Parrish et al, 2007). The agreement between different methods applied to the same genome is not particularly good, although in a favorable case of 31 proteins from the H. pylori type IV secretion system (Terradot et al, 2004) co-purification experiments authenticated 76% of the interactions predicted from large-scale Y2H experiments (Rain et al, 2001).…”

Chemotaxis inCampylobacter Jejuni

“…The varying binding strength of CheV and CheW to different chemoreceptor signalling domains suggests that both proteins may out-compete each other depending on the specific receptor and the protein concentration [33,66]. Thus, CheW preferentially binds Tlp4, whereas CheV demonstrates a high affinity to Tlp1, Tlp4, Tlp6 as well as Tlp8 [65,66].…”

“…Thus, CheW preferentially binds Tlp4, whereas CheV demonstrates a high affinity to Tlp1, Tlp4, Tlp6 as well as Tlp8 [65,66]. It is possible that this competition is part of a kind of sensory adaptation.…”