A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulated pathway.

Possenti, Roberta; Eldridge, Juanita; Paterson, Bruce M.; Grasso, A.; Levi, Andrea

doi:10.1002/j.1460-2075.1989.tb08345.x

Cited by 102 publications

(97 citation statements)

References 35 publications

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“…The present result provides a further clear-cut example of a differential regulation of these peptides costored within the same organelle [30]. An analogous behaviour to secretogranin II has recently been found for another protein apparently stored in large dense core vesicles of PC12 cells [31].…”

Section: Discussionsupporting

confidence: 80%

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

et al. 1990

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Human neuroblastoma cells were cultured either in the absence or presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) known to induce neuronal differentiation. This treatment led to a marked increase in the concentration of secretogranin II but to a decrease of chromogranin A. Analogous changes were observed for the respective mRNAs. Thus during differentiation of these cells the biosynthesis of two vesicle constituents of large dense core vesicles is differentially regulated as determined both at the mRNA and the protein level. Levels of both synaptin/synaptophysin and SV2 were also elevated but to a smaller degree than that of secretogranin II.

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Section: Discussionsupporting

confidence: 80%

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

et al. 1990

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“…This result suggests that di erentiation is dependent upon reasonable amounts of trk being expressed by the cells. To determine if the morphological di erentiation observed at 328C in PC12trk ts p53 cells in the absence of NGF stimulation had characteristics similar to NGF mediated di erentiation, the induction of a speci®c protein marker for di erentiation, VGF, was examined (Possenti et al, 1989). Double transfectants grown at 328C were positive for VGF-antigen immunostaining in a pattern similar to that induced by NGF treatment (Possenti et al, 1989) in control PC12trk cells (data not shown).…”

Section: Analysis Of Pc12 Cells Expressing Trk and Ts P53mentioning

confidence: 95%

p53 associates with trk tyrosine kinase

Montano

1997

Oncogene

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The p53 tumor suppressor gene encodes a phosphoprotein which when overexpressed can induce growth arrest at the G1 and G2/M phases of the cell cycle, promote di erentiation and apoptosis. This paper demonstrates that p53 can associate with trk tyrosine kinase. Expression of a murine temperature-sensitive (ts) p53 mutant in PC12 cells overexpressing trk (a model system to analyse cellular di erentiation and signal transduction induced by NGF) induces morphological changes in the absence of NGF stimulation at 328C but not at 378C. In cells di erentiated by p53, trk, but not EGFr, was hyperphosphorylated on tyrosine. Furthermore trk was not phosphorylated when expressed in Saos-2 cells (human osteosarcoma cells that lack expression of both endogenous trk and p53) at either temperature. However, transfection of ts p53 into these cells induces trk phosphorylation at 328C in the absence of NGF stimulation. Association of trk and p53 can be detected in NIH3T3 and PC12 cells co-expressing trk and the ts p53 mutant, in NIH3T3 and PC12 cells transfected with trk alone, and in untransfected PC12 cells, showing that overexpressed and/or endogenous trk associates with endogenous, low levels of p53. These data suggest a novel function for p53 which involves the stimulation of signal transduction pathways (mediating morphological properties of cells), possibly through association with and hyperphosphorylation of trk.

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“…Chromogranin B is detectable in many of the cells (70%) and shows an even distribution over the cytoplasm. Another secretory protein, (VGF8) (Possenti et al, 1989) was instead absent.…”

mentioning

confidence: 99%

Immortalization of neuro-endocrine cells from adrenal tumors arising in SV40 T-transgenic mice

Cairns¹,

Crotta

Minuzzo

et al. 1997

Oncogene

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A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulated pathway.

Cited by 102 publications

References 35 publications

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

p53 associates with trk tyrosine kinase

Immortalization of neuro-endocrine cells from adrenal tumors arising in SV40 T-transgenic mice

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