A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species and tissues

Hertzberg, Elliot L.; Skibbens, Robert V.

doi:10.1016/0092-8674(84)90191-0

Cited by 146 publications

(70 citation statements)

References 43 publications

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“…Cell lysates were prepared by alkaline treatment to enrich for integral membrane proteins according to the procedure as described (29). One microgram of cell lysate proteins was separated by 10% SDS-PAGE and transferred onto an Immobilon membrane (Millipore Corp., Bedford, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

Ito¹,

Katoh²,

Kataoka³

et al. 2000

J. Clin. Invest.

170

188

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Section: Methodsmentioning

confidence: 99%

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

Ito¹,

Katoh²,

Kataoka³

et al. 2000

J. Clin. Invest.

170

188

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“…Conditions for SDS-PAGE and Western blots were as described previously. 24,25 Samples were resolved on 10% gels and electrophoretically transferred to nitrocellulose membranes. The rabbit anti-Cx43 antibody used [20][21][22] was generated to a sequence corresponding to amino acids 346-360 of Cx43.…”

Section: Methodsmentioning

confidence: 99%

Deficient assembly and function of gap junctions in Trf1, a trafficking mutant of the human liver-derived cell line HuH-7

et al. 1999

Self Cite

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Gap junctions are complexes between adjacent cells that are responsible for direct intercellular communication via passive diffusion of low-molecular-weight hydrophilic molecules. The proteins present in gap junctions are termed connexins, of which at least 16 distinct gene products are known to occur in vertebrate species. 1,2 Connexin proteins differ notably from other membrane proteins in lacking glycosylation and being localized primarily at appositional regions between coupled cells. While the pathways of biogenesis, trafficking, assembly, and turnover have not yet been completely elucidated, key features appear to be more similar than not to ''typical'' plasma membrane proteins. [3][4][5][6][7][8][9][10][11] In previous studies, biochemical analysis of secretory or trafficking mutants 12-14 has elucidated many features of these pathways. This approach for analysis of gap junction biology has not been pursued in vertebrate cell lines harboring similar mutations. Characterization of connexin trafficking in the membrane protein trafficking mutant, Trf1, was undertaken as a first step in combining biochemical and mutational approaches to analysis of connexin trafficking and assembly.The Trf1 cell line was isolated as a result of a dual selection protocol. 15 Endocytic activity of 2 different carbohydratespecific receptors of the human hepatoma cell line HuH-7 were simultaneously selected against using gelonin, an inhibitor of protein synthesis, conjugated to galactose-and mannoseterminating glycoproteins. Because there was no significant reciprocal inhibition between the mannose-and galactoseterminating glycoproteins and their respective gelonin conjugates, the mutation harbored by Trf1 cells appeared to affect a common step in their endocytic pathway. This pleiotropic phenotype was extended to other cell-surface membrane proteins, including the transferrin receptor and the phenylalanine transporter.The Trf1 mutation provided the first genetic evidence for the existence of different subpopulations (States 1 and 2) of the asialoglycoprotein receptor (ASGPR) as described by Weigel and Oka. 16 Originally based on the biphasic kinetics of ligand-receptor complex dissociation, numerous characteristics have since been described that differentiate the 2 ASGPR subpopulations and the proposed endocytic pathways that they mediate. 17 While no biochemical basis for this receptor dichotomy has been established, only State 2 receptors were shown to be susceptible to modulation by a wide variety of cell perturbants and altered metabolic states of the liver. 17 The proposed existence of 2 subpopulations of receptors is not limited to ASGPR and has been suggested for a wide variety of cell-surface receptors on different cell types. The low-density lipoprotein and mannose-6-P receptors on fibroblasts, the mannose receptor and ␣ 2 macroglobulin receptor on alveolar macrophages, and the transferrin receptor on K562 and HuH-7 cells all respond to cell perturbants by a partial reduction in their surface expression, suggesting Abb...

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“…They belong to the most conserved structures in multicellular metazoans and can be found from hydra to man (Filshie and Flower 1977;Hertzberg and Skibbens 1984;Bauer et al 2005;Fraser et al 1987). Gap junctions are present between most cell types and actively participate in many aspects of the cellular life cycle, ranging from cell growth to cell death (for recent reviews see Vinken et al 2006;Meier and Dermietzel 2006;Leithe et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Molecular Diversity of Connexin and Pannexin Genes in the Retina of the ZebrafishDanio rerio

Zoidl

Kremer

Zoidl

et al. 2008

Cell Communication & Adhesion

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Gap junctions are among the most widely distributed cell structures involved in cell-to-cell communication. Recently completed genome sequencing projects including species from all major phyla have demonstrated the existence of three distinct gene families, the connexins, pannexins, and innexins, as molecular building blocks of gap junctional communication. In the present study, the authors have addressed the molecular complexity of gap junction gene expression in the zebrafish retina, a remarkably complex sensory organ built by diverse neuronal subtypes. Using a combination of cDNA library and genomic DNA library screening and/or RACE technology, the authors have cloned, in addition to the four previously reported connexins, seven novel connexins and four pannexin transcripts resembling two pannexin genes. This result demonstrates the presence of two distinct gap junction type gene families and indicates a remarkable molecular and functional diversity of gap junction-mediated coupling in the fish retina.

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A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species and tissues

Cited by 146 publications

References 43 publications

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

Deficient assembly and function of gap junctions in Trf1, a trafficking mutant of the human liver-derived cell line HuH-7

Molecular Diversity of Connexin and Pannexin Genes in the Retina of the ZebrafishDanio rerio

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