2016
DOI: 10.1016/j.bjm.2016.01.026
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A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri

Abstract: Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is usually achieved by the elimination of affected plants along with that of asymptomatic neighbors. An in depth understanding of the pathogen is the keystone for understanding of the disease; to this effect we are committed to the development of strategies to ease the study… Show more

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Cited by 3 publications
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“…Subsequently, the culture was centrifuged for 15 min at 4,500 rpm at room temperature and the bacterial pellet was resuspended in 25 mL of lysis buffer (10 mM Tris base, 1 M NaCl, Triton X-100 (0.05%, pH 8.0)). Cellular contents were lysed on ice through 2 cycles of ultrasonication (Vibracell ® VC100, Sonic and Materials Inc.) with an interval of 30 s, adapted from Dantas et al [ 37 ]. Bacterial lysates were centrifuged for 15 min at 10,500 rpm in order to separate soluble contents from insoluble cell debris.…”
Section: Methodsmentioning
confidence: 99%
“…Subsequently, the culture was centrifuged for 15 min at 4,500 rpm at room temperature and the bacterial pellet was resuspended in 25 mL of lysis buffer (10 mM Tris base, 1 M NaCl, Triton X-100 (0.05%, pH 8.0)). Cellular contents were lysed on ice through 2 cycles of ultrasonication (Vibracell ® VC100, Sonic and Materials Inc.) with an interval of 30 s, adapted from Dantas et al [ 37 ]. Bacterial lysates were centrifuged for 15 min at 10,500 rpm in order to separate soluble contents from insoluble cell debris.…”
Section: Methodsmentioning
confidence: 99%