A preliminary study on the genetic profile of cag pathogenicity-island and other virulent gene loci of Helicobacter pylori strains from Turkey

Salih, Barik A.; Abasıyanık, M. Fatih; Ahmed, Niyaz

doi:10.1016/j.meegid.2007.03.002

Cited by 32 publications

(33 citation statements)

References 20 publications

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“…In accordance with the previous reports from Turkey, we found vacA s1a as the predominant vacA s subtype with a rate of 70.1% (7,13,24). According to the reports from East Asian countries, all H. pylori strains isolated from East Asian countries are vacA s1 and cagA-positive, where the gastric cancer incidence has been reported in higher rates (25).…”

Section: Resultssupporting

confidence: 93%

“…Thus, in the present study we investigated the cagA prevalence and vacA allelic subtypes retrospectively and evaluated their relationships with clinical outcomes in strains that had been isolated at Hacettepe University Hospital. Table 5 summarises the vacA s and m genotype rates in the present study and in other studies from Turkey (7,(12)(13)(14)(15)(16)(17). It has been known that cagA positivity rates and their association with clinical outcomes differ from region to region.…”

Section: Resultsmentioning

confidence: 65%

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Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Erdoğdu

Sarıbaş²,

Yilmaz³

2014

Turk J Med Sci

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The cagA and vacA profiles and their association with clinical findings show a distinct geographical distribution. In the present study, we aimed to determine the cagA status and vacA allelic subtypes in strains isolated from a university hospital in Ankara and to evaluate their associations with histopathological and endoscopic findings. Materials and methods:A total of 120 H. pylori strains from stock cultures positive for the ureA gene were randomly included in the present study. Of these strains, cagA and vacA allelic subtypes (s1a, s1b, s2, m1, m2) were examined by polymerase chain reaction.Results: Of the 120 strains, 64 (53.3%) were cagA-positive. However, no significant relationship was found between clinical outcomes and cagA positivity. There were 38 (33.6%) strains that had vacA m1 and 74 (65.5%) that had vacA m2 region. Overall, 75 (70.1%) samples were classified as vacA s1a, 3 (2.8%) as vacA s1b, and 29 (27.1%) as vacA s2. There was no significant relationship between vacA genotypes and endoscopic findings. The predominant vacA genotypes were s1am2 (35.6%) and s1am1 (33.6%), with almost the same rates. Furthermore, cagA positivity was found to be significantly related with the vacA s1am1 genotype. Conclusion:The cagA and vacA profiles of our study population are consistent with the Middle Eastern profile.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 65%

Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Erdoğdu

Sarıbaş²,

Yilmaz³

2014

Turk J Med Sci

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“…In the present study, the vacA gene was observed in all strains. Our finding was similar to previous reports from Turkey (10,25,26), Northern and Eastern European countries (32) where s1a gene was predominant genotype, but in contrast to a report from Korea (16). We detected a low prevalence of vacAs1b in this study which was contrast to pevious reports in Portugal, Central and South America (32).…”

Section: H Pylori Genotypessupporting

confidence: 92%

“…The prevalence (35.7%) of cagE in this study is lower than other studies conducted in USA (64%) (22), Turkey (59.3%) (10), UK (71.2%) (15) and Thailand (88.4%) (8) but higher than a previous study in Turkey (28.6%) (25). In an attempt to detect association between the cagE gene and PU, In contrast to the results reported in China, Japan, Korea and Netherlands where the iceA1 gene was predominant (16,20,31), the iceA2 gene was detected to be predominant genotype in this study.…”

Section: H Pylori Genotypescontrasting

confidence: 82%

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Ozbey¹,

Aygün²

2012

Braz. J. Microbiol.

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There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacAs1, vacAs2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacAs1m1 (65.2%) and s1m2 (53.9%) in patients with peptic ulcer and gastritis, respectively.The only vacAs1 isolate was significantly associated with gastritis and peptic ulcer (p<0.05). The vacAs1a, ml, slml and babA2 genes were significantly associated with peptic ulcer (p<0.05), whereas m2 gene was significantly associated with only gastritis (p<0.05). The difference between sex and genotypes was statistically significant among the cagA, vacAs1, iceA2 and babA2 genes. This study reported for the first time the prevalence of H. pylori genotypes in patients with gastrointestinal complaints in eastern Turkey.Further studies are needed to understand epidemiological importance of the genotypes of H. pylori isolates in this region and the association between the virulence genes and clinical outcome in different regions.

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“…It is known that H pylori infection with cytotoxin-associated (CagA) gene positive strains has been related with increased risk for development peptic ulcer and gastric carcinoma (Blaser et al, 1995;Parsonnet et al, 1997). Recent studies demonstrated the association with cagA positive H pylori and the higher rate for development gastroduodenal disease, in Turkish population (Sezikli et al, 2006;Salih et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Clinical Factors Related with Helicobacter Pylori Infection - Is there an Association with Gastric Cancer History in First-Degree Family Members?

Demirel¹,

Akkaş²,

Vural³

2013

Asian Pacific Journal of Cancer Prevention

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A preliminary study on the genetic profile of cag pathogenicity-island and other virulent gene loci of Helicobacter pylori strains from Turkey

Cited by 32 publications

References 20 publications

Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Detection of cagA and vacA genotypes of Helicobacter pylori isolates from a university hospital in Ankara region, Turkey

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Clinical Factors Related with Helicobacter Pylori Infection - Is there an Association with Gastric Cancer History in First-Degree Family Members?

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