A preliminary study of the anti-inflammatory and anti-apoptotic effects of crocin against gastric ischemia-reperfusion injury in rats

Abstract

The aim of the present study was to investigate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats. Thirty-two male rats were randomly divided into sham, I/R, I/R + crocin pretreatment and crocin alone groups. To induce I/R lesions, the celiac artery was clamped for 30 min, and the clamp was then removed to allow reperfusion for 3 h. Crocin-pretreated rats received crocin (15 mg/kg, i.p.) 30 min prior to the induction of I/R injury. Samples of gastric mucosa were collected to quantify the protein expression of caspase-3, an apoptotic factor, and inducible nitric oxide synthase (iNOS), a pro-inflammatory protein, by Western blot. Pretreatment with crocin decreased the total area of gastric lesions and decreased the protein expression levels of caspase-3 and iNOS induced by I/R injury. Our findings showed a protective effect of crocin in gastric mucosa against I/R injury. This effect of crocin was mainly mediated by reducing the protein expression of iNOS and caspase-3. Uniterms:Crocin/protective effect. Gastric mucosal/lesions/ ischemia-reperfusion. Gastric lesions/ treatment/experimental study. Caspase-3. Inducible nitric oxide synthase.O objetivo do presente estudo foi investigar o efeito protetor da crocina em lesões da mucosa gástrica causadas por isquemia-reperfusão (I/R) em ratos. Trinta e dois ratos machos aleatoriamente divididos em grupos de ratos normais, operados como controle, I/R. I/R + pré-tratamento com crocina e crocina sozinha. Para induzir lesões I/R, a artéria celíaca foi grampeada durante 30 minutos e, em seguida, o grampo foi removido para permitir a reperfusão por 3 h. Ratos com pré-tratamento com crocina receberam crocina (15 mg/kg, ip) 30 minutos antes da indução do dano I/R. Amostras de mucosa gástrica foram coletadas para qiuantificar a expressão da proteína da caspase-3, o fator apoptótico, e óxido nítrico sintase induzível (iNOS), uma proteína anti-inflamatória, pela técnica de Western Blot. O pré-tratamento com crocina diminuiu a área total de lesões gástricas e a expressão de níveis de caspase-3 e iNOS induzidas pelo dano I/R. Nossos resultados mostraram o efeito protetor da crocina na mucosa gástrica contra o dano I/R. Este efeito foi mediado, principalmente, por diminuição da expressão das proteínas iNOS e caspase-3.Unitermos: Crocina/efeito protetor. Mucosa gástrica/lesão/isquemia-reperfusão. Lesões gástricas/ tratamento/estudo experimental. Caspase-3. Óxido nítrico sintase induzível.

The effect of metyrosine on oxidative gastric damage induced by ischemia/reperfusion in rats. Biochemical and histopathological evaluation

“…Previous studies have shown that microscopic changes such numerous erosions, spillage of superficial cells, necrosis, hemorrhages in the mucosal layers, inflammation, and neutrophil aggregation occur in gastric tissue with the I/R procedure, and that administration of gallic acid reduces these symptoms 28 . In our study, metyrosine had a protective effect on gastric tissue by preventing histopathological symptoms such as necrosis, hemorrhage, and edema that developed in the stomach due to I/R injury.…”

The effect of hydroalcoholic Saffron (Crocus sativus L.) extract on fasting plasma glucose, HbA1c, lipid profile, liver, and renal function tests in patients with type 2 diabetes mellitus: A randomized double‐blind clinical trial

“…Various pharmacological effects of saffron and its components have been published in some studies, such as antidepressant (Akhondzadeh et al, 2005;Moshiri et al, 2006), Alzheimer's treatment (Akhondzadeh et al, 2010) anticancer (Abdullaev & Espinosa-Aguirre, 2004;Bolhassani, Khavari, & Bathaie, 2014), anti-ischemic on the kidneys (Hosseinzadeh & Nassiri-Asl, 2013), brain (Naghizadeh, Mansouri, Ghorbanzadeh, Farbood, & Sarkaki, 2013), heart (Bharti, Golechha, Kumari, Siddiqui, & Arya, 2012), and muscle (Hosseinzadeh, Modaghegh, & Saffari, 2009). In addition, analgesic and antiinflammatory effects have been reported in various studies (Mard, Nikraftar, Farbood, & Mansouri, 2015;Nasri, Hosseini, Sahraei, & Zardooz, 2011;Rezaee & Hosseinzadeh, 2013).…”

Therapeutic potency of crocin in the treatment of inflammatory diseases: Current status and perspective

“…L E 1 Modulatory effects of crocin on inflammatory diseases Decreasing the mRNA expression of IFN-γ, TNFα, IL-1β, COX-2, NF-κB Mard et al (2015). …”