A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis

Abstract

Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods.

The Pseudomonas aeruginosa Complement of Lactate Dehydrogenases Enables Use of d - and l -Lactate and Metabolic Cross-Feeding

“…Similarly, other common CF pathogens encode l -lactate dehydrogenases homologous to LldD and LldA. Using the P. aeruginosa PA14 LldD, LldA, and LldE protein sequences, we searched for homologues in representative strains of Achromobacter xylosoxidans , Burkholderia multivorans , Haemophilus influenzae , and Stenotrophomonas maltophilia ( 33 – 35 ). We found that H. influenzae and S. maltophilia contain an l -lactate dehydrogenase homolog but that A. xylosoxidans and B. multivorans , like P. aeruginosa , contain multiple homologs.…”