A practicable method for the analysis of T lymphocyte subsets in CSF lymphopleocytosis

Abstract

A practicable indirect immunofluorescence technique for the analysis of surface markers of cerebrospinal fluid (CSF) cells is described. Preliminary results in 10 patients with acute or chronic CSF lymphopleocytosis of different etiology showed an almost uniform pattern of cell subsets. The majority of CSF cells represented OKT3-positive T lymphocytes with a mean helper (OKT4-positive) to suppressor (OKT8-positive) ratio of 3,4 to 1.

A reliable method for immunocytochemical characterization of CSF cells

“…With all the mAbs used (see Materials and Methods), the immunoenzymatic staining method yielded results similar to those obtained with our previously described indirect immunofluorescence technique [4] (unpublished observations). This is in accordance with observations that immunoenzyme staining of stored prefixed preparations of peripheral blood cells gives the same results as indirect immunofluorescence staining of blood cell suspensions [7].…”