A potential probiotic- Lachnospiraceae NK4A136 group: Evidence from the restoration of the dietary pattern from a high-fat diet

Abstract

Background: High-fat diet (HFD) that contributes to obesity is one of the pivotal risk factors for metabolic syndrome and cancers. The dietary pattern can shape the intestinal bacterial community and influence the physiological parameters. This study aimed to investigate whether the short-term dietary pattern shift from HFD to a balanced chow diet (CD) could correct HFD-induced colonic dysbiosis and reverse adverse health effects and identify the specific bacteria that changed by dietary patterns. Results: C57BL/6 mice fed with an HFD for 10 months, followed by a CD for 3 months, served as the dietary shift model. Stool samples were collected for bacterial analysis. Physiological parameters, such as serum adipokines, blood lipid levels, and hepatic function, were monitored in control and dietary shift groups. HFD-induced weight gain was mitigated by the dietary shift. A highly similar structure at the phylum, genus, and species levels was observed in the beta diversity of colonic bacteria in mice that underwent the dietary shift as compared to those in the control group. Notably, the abundance of Peptococcaceae and Akkermansiaceae in HFD-fed mice reduced after the dietary shift; whereas the diminished amount of probiotic Lachnospiraceae NK4A136 group in HFD-fed mice was restored to the level comparable to those in controls after the dietary shift. Conclusions: Our finding suggests that a dietary switch from a long-term HFD to a short-term balanced diet has the potential to correct colonic dysbiosis and restore physiological homeostasis. The Lachnospiraceae NK4A136 group has the potential to be a probiotic.

Altering Methane Emission, Fatty Acid Composition, and Microbial Profile during In Vitro Ruminant Fermentation by Manipulating Dietary Fatty Acid Ratios

“…In addition, the HN6 group displayed the lowest relative abundance of Lachnospiraceae NK4A136 and the highest relative abundance of Ruminiclostridium_6. Lachnospiraceae NK4A136 is considered to be a potential probiotic and exerts anti-inflammation effects [87]. Higher Ruminiclostrid-ium_6 abundance was positively correlated with cytokine levels [88].…”

Gut microbiota-based vaccination engages innate immunity to improve blood glucose control in obese mice

“…The abundance of Lachnospiraceae.NK4a136.group was significantly increased in the ileum and colon of vaccinated mice. Lachnospiraceae.NK4a136.group is a potential butyrate-producer associated with metabolic fitness and gut homeostasis [ 60 , 61 ] and probiotic activity in mice [ 62 ]. We found that Lachnospiraceae.NK4a136.group constituted ∼20% of the sequenced bacterial taxa in vaccinated mice, which makes it a strong candidate for future testing related to glycemic control.…”

Black Tea Alleviates Particulate Matter-Induced Lung Injury via the Gut-Lung Axis in Mice

“…52 Furthermore, the Lachno-spiraceae_NK4A136_group was supposed to play a probiotic role in the mouse obesity model. 53,54 Therefore, the Lachnospiraceae_NK4A136_group, the core beneficial bacteria regulated by EP, is suggested to play a crucial role in protecting mice from PM-induced lung injury. Besides, it can be observed that the genera of bacteria regulated by TI and ES were different from those regulated by EP, which may partially explain why TI (a combination of ES and EP) showed weaker protection than EP or ES, but further investigation on the interaction between microbiota modulated by ES and EP is needed to confirm this hypothesis.…”