A polycaprolactone/fish collagen/alginate biocomposite supplemented with phlorotannin for hard tissue regeneration

Abstract

In tissue engineering, scaffolds have been widely studied for the purpose of regenerating damaged tissues or organs. Biocompatibility of materials, pore structure, and even growth factors are effective for enhancing cell growth and differentiation. In particular, mammalian collagen is biocompatible and hydrophilic, and provides good cell attachment due to interactions between Arg-Gly-Asp (RGD) domains and cell membranes. However, there have been some limitations on the use of mammalian collagen. In this study, we present a biocomposite scaffold supplemented with collagen extracted from fish skin and phlorotannin from brown algae. The effect of these biocomponents on various physical characteristics, including water wetting ability and tensile properties, was observed. The in vitro cellular activities of the biocomposites were examined using osteoblast-like cells (MG63) to assess cell proliferation and mineralization. The results show that the cells were more highly proliferated on the phlorotannin/fish collagen/alginate biocomposite compared to the control (collagen/alginate). Furthermore, the biocomposite showed significantly higher in vitro calcium deposition (38.37%) and osteogenesis (43.2%), determined by histomorphometric analysis, than the control. Based on these results, the biocomposite supplemented with fish collagen/phlorotannin was demonstrated to be a good potential biomaterial for enhancing bone tissue growth.

Seaweed Phenolics: From Extraction to Applications

“…Tissue and bone engineering is a new biomedical area where the phlorotannins are recently being tested [321]. This area focuses on the regeneration of damaged tissues, bones, or organs where biomedical scaffolds, cells, and growth factors are combined to obtain success [322].…”

“…A study by Im et al [321] demonstrated that the insertion of phlorotannins on the fish collagen/alginate biocomposite obtained a better result in the cell proliferation. In the in vitro assay, this new mixture showed better results in calcium deposition and osteogenesis, demonstrating that this product with phlorotannins is a potential biomaterial for the bone tissue growth.…”

In Situ Cross-Linked Collagen-Based Biological Patch Integrating Anti-Infection and Anti-Calcification Properties

“…Biological materials with proper hydrophilicity would be more favorable for cell growth. 44,45 From Figure 2D, the WCA of pADM was about 65°, and the WCA of 8MA−pADM was not significantly different from that of pADM. However, with the increase of DMC dosage, the contact angle of the material gradually decreased, that is, the hydrophilicity gradually increased.…”

Natural Phenol Polymers: Recent Advances in Food and Health Applications

“…A biomedical scaffold should have a three-dimensional shape that is structurally and mechanically equivalent to the replaced tissue and should provide attractive sites for the injected cells to attach, proliferate and differentiate. Biocomposite scaffolds based on polycaprolactone supplemented with hydrophilic collagen extracted from fish skin and phlorotannins from brown algae exhibited marked calcium deposition and osteogenesis ability compared to the materials not including the polyphenols [ 180 ]. Micro-/nano-fibrous substrates were also widely used for tissue regeneration because of their similarities to extracellular matrix components and their high surface area, which facilitates cell attachment and proliferation.…”