A point-of-care rapid HIV-1 test using an isothermal recombinase-aided amplification and CRISPR Cas12a-mediated detection

Evaluation of an automated CRISPR-based diagnostic tool for rapid detection of COVID-19

“…For example, CRISPR-Cas12a technology was evaluated in the diagnosis of human papillomavirus, and the data suggested that this tool could achieve results within 35 min with a low detection limit [ 33 ]. An integrated assay including RT-RPA and CRISPR Cas12a-mediated detection was evaluated in the diagnosis of HIV-1 infection, showing a sensitivity of 98.95% and specificity of 100%, respectively [ 34 ]. Similarly, this combination showed a high sensitivity (93.75%) and specificity (90.63%) for the detection of Yersinia pestis , as reported by You et al [ 35 ].…”

Recent HIV Infection: Diagnosis and Public Health Implications

“…Other diverse approaches are also being investigated, including circulating cellular microRNAs in plasma as biomarkers of HIV infection, the detection of recent HIV infection based on naturally inspired synthetic oligomers, antibody reactivity to a panel of different HIV peptides that can better predict the duration of HIV infection, studying the genetic diversity of HIV during the early period of infection, which is expected to be less than that in later stages, POC rapid testing of HIV-1 RNA or DNA using new technologies, the use of gold nanocluster immunoassays, etc. [ 31 , 34 , 67 , 68 , 69 , 70 , 145 , 146 , 147 , 148 , 149 , 150 ]. The World Health Organization (WHO) long ago set up a technical group on HIV incidence assays.…”

The CRISPR-Cas system as a tool for diagnosing and treating infectious diseases

“…The regulation of CRISPR-Cas9 genome editing on HIV-1 genome has become an effective tool for CRISPR-Cas9 system to treat HIV-1 [ 79 ]. Using a combination of isothermal amplification and CRISPR-Cas12a-mediated detection, HIV-1 genotypes were detected in 30 min under non-laboratory conditions [ 80 ]. Tuberculosis (TB) is difficult to diagnose quickly.…”