A photostable fluorescent probe for targeted imaging of tumour cells possessing integrin αvβ3**

Xiong, Liqin; Yu, Mengxiao; Cheng, Mingjun; Zhang, Meng; Zhang, Xiaoyan; Xu, Congjian; Li, Fuyou

doi:10.1039/b820576k

Cited by 28 publications

(20 citation statements)

References 30 publications

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“…It is known that HeLa cells have at least 2-3 RGD-specific integrins, α 5β148, α vβ54849 and α vβ34950. α vβ3 is overexpressed in HeLa cells and, being a critical factor for angiogenesis, its expression level is a general measure of the malignancy of tumorous cells51.…”

Section: Resultsmentioning

confidence: 99%

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Orgován

Péter

Bősze

et al. 2014

Sci Rep

105

192

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A novel high-throughput label-free resonant waveguide grating (RWG) imager biosensor, the Epic® BenchTop (BT), was utilized to determine the dependence of cell spreading kinetics on the average surface density (vRGD) of integrin ligand RGD-motifs. vRGD was tuned over four orders of magnitude by co-adsorbing the biologically inactive PLL-g-PEG and the RGD-functionalized PLL-g-PEG-RGD synthetic copolymers from their mixed solutions onto the sensor surface. Using highly adherent human cervical tumor (HeLa) cells as a model system, cell adhesion kinetic data of unprecedented quality were obtained. Spreading kinetics were fitted with the logistic equation to obtain the spreading rate constant (r) and the maximum biosensor response (Δλmax), which is assumed to be directly proportional to the maximum spread contact area (Amax). r was found to be independent of the surface density of integrin ligands. In contrast, Δλmax increased with increasing RGD surface density until saturation at high densities. Interpreting the latter behavior with a simple kinetic mass action model, a 2D dissociation constant of 1753 ± 243 μm−2 (corresponding to a 3D dissociation constant of ~30 μM) was obtained for the binding between RGD-specific integrins embedded in the cell membrane and PLL-g-PEG-RGD. All of these results were obtained completely noninvasively without using any labels.

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Section: Resultsmentioning

confidence: 99%

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Orgován

Péter

Bősze

et al. 2014

Sci Rep

105

192

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“…As far as the biological imaging is concerned, the nding is an interesting and useful feature of the UCNPs, 50 which has not been obtained in the traditional single-photon or two-photon uorescent imaging. [55][56][57][58][59][60][61][62][63][64][65][66] While excited with the 980 nm laser light the biological samples presented weak absorption with no luminescence in the visible region. Fig.…”

Section: View Article Onlinementioning

confidence: 99%

Upconversion fluorescence imaging of HeLa cells using ROS generating SiO₂-coated lanthanide-doped NaYF₄ nanoconstructs

et al. 2017

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Inorganic nanomaterials able to generate reactive oxygen species (ROS) are promising components for modern medical applications. Activated by near-infrared light, up-converting b-NaYF 4 doped with Er 3+ -Yb 3+ and Tm 3+ -Yb 3+ pair ions nanoparticles (UCNPs), have a wide range of applications in biological imaging as compared to traditional reagents excited by ultra-violet or visible light. We analysed the green-red and the blue-red luminescence to explain the mechanism of the upconversion depended on the surface condition. The influence of SiO 2 coating on the cytotoxicity of the as-produced UCNPs towards HeLa cancer cells was reported. We demonstrated a possibility of a direct UCNPs application to photodynamic therapy, without need to attach additional molecules to their surface. The presence of Tm 3+ -Yb 3+ pair ions, thus ROS generation capability, renders the SiO 2 shell coated nanoparticles to become potentially useful theranostic agent.

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“…The reaction of 1 with 3-thiopropionic acid has used to produce a fluorescent sensor for cys/Hcy with a 75-fold fluorescence enhancement [34]. The S N Ar H reaction of 1 with the thiolated RGD peptide cyclo(Arg-Gly_Asp-Phe-Lys(mpa))(c(RGDFK)-SH) leads to a fluorescent sensor for imaging tumor cells [37]. …”

Section: Introductionmentioning

confidence: 99%

Chromo- and fluorophoric water-soluble polymers and silica particles by nucleophilic substitution reaction of poly(vinyl amine)

Hofmann¹,

Kahle²,

Simon³

et al. 2010

Beilstein J. Org. Chem.

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SummaryNovel chromophoric and fluorescent carbonitrile-functionalized poly(vinyl amine) (PVAm) and PVAm/silica particles were synthesized by means of nucleophilic aromatic substitution of 8-oxo-8H-acenaphtho[1,2-b]pyrrol-9-carbonitrile (1) with PVAm in water. The water solubility of 1 has been mediated by 2,6-O-β-dimethylcyclodextrin or by pre-adsorption onto silica particles. Furthermore, 1 was converted with isopropylamine into the model compound 1-M. All new compounds were characterized by NMR, FTIR, UV–vis and fluorescence spectroscopy. The solvent-dependent UV–vis absorption and fluorescence emission band positions of the model compound and the carbonitrile-functionalized PVAm were studied and interpreted using the empirical Kamlet–Taft solvent parameters π * (dipolarity/polarizability), α (hydrogen-bond donating capacity) and β (hydrogen-accepting ability) in terms of the linear solvation energy relationship (LSER). The solvent-independent regression coefficients a, b and s were determined using multiple linear correlation analysis. It is shown, that the chains of the polymer have a significant influence on the solvatochromic behavior of 1-P. The structure of the carbonitrile 1-Si bound to polymer-modified silica particles was studied by means of X-ray photoelectron spectroscopy (XPS) and Brunauer–Emmett–Teller (BET) measurements. Fluorescent silica particles were obtained as shown by fluorescence spectroscopy with a diffuse reflectance technique.

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A photostable fluorescent probe for targeted imaging of tumour cells possessing integrin αvβ3**

Cited by 28 publications

References 30 publications

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Upconversion fluorescence imaging of HeLa cells using ROS generating SiO₂-coated lanthanide-doped NaYF₄ nanoconstructs

Chromo- and fluorophoric water-soluble polymers and silica particles by nucleophilic substitution reaction of poly(vinyl amine)

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A photostable fluorescent probe for targeted imaging of tumour cells possessing integrin αvβ3**

Cited by 28 publications

References 30 publications

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Upconversion fluorescence imaging of HeLa cells using ROS generating SiO2-coated lanthanide-doped NaYF4 nanoconstructs

Chromo- and fluorophoric water-soluble polymers and silica particles by nucleophilic substitution reaction of poly(vinyl amine)

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Product

Resources

About

Upconversion fluorescence imaging of HeLa cells using ROS generating SiO₂-coated lanthanide-doped NaYF₄ nanoconstructs