volume 48, issue 4, P405-414 2007
DOI: 10.1002/jctb.280480403
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Abstract: Abstract A fed‐batch culture of Leuconostoc mesenteroides has been operated from an initial volume of 4 dm3 using a sucrose/NaOH solution feed on demand with pH control. An enzyme concentration of greater than 300 DSU cm−3 was achieved in 15 h. A yield of 0.107 kg cells kg−1 sucrose feed was determined. The enzyme production was growth associated at 2.67 × 107 DSU kg−1 cells produced. Growth was shown to be linear and attributed to an unknown conservative trace nutrient limitation associated with the yeast ex…

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