A PCR Survey of Hox Genes in the Sea Star,Asterina minor

Mito, Taro; Endo, Kazuhiko

doi:10.1006/mpev.1997.0417

Cited by 23 publications

(25 citation statements)

References 29 publications

(34 reference statements)

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“…The asteroid also has a single Hox gene cluster (Mito and Endo, 1997). These results strongly suggest that possession of a single cluster is a common feature of echinoderms.…”

Section: Introductionmentioning

confidence: 89%

PCR Survey of Hox Genes in the Crinoid and Ophiuroid: Evidence for Anterior Conservation and Posterior Expansion in the Echinoderm Hox Gene Cluster

Mito

Endo

2000

Molecular Phylogenetics and Evolution

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“…The asteroid also has a single Hox gene cluster (Mito and Endo, 1997). These results strongly suggest that possession of a single cluster is a common feature of echinoderms.…”

Section: Introductionmentioning

confidence: 89%

PCR Survey of Hox Genes in the Crinoid and Ophiuroid: Evidence for Anterior Conservation and Posterior Expansion in the Echinoderm Hox Gene Cluster

Mito

Endo

2000

Molecular Phylogenetics and Evolution

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“…Genomic DNA was extracted basically following the procedure of Mito & Endo (1997). Contamination of the DNA from extraneous material was prevented, as much as possible, by rinsing the dissected tissues several times with ultrapure water prior to DNA extraction.…”

Section: Amplification Of Hox Gene Fragmentsmentioning

confidence: 99%

“…The previously described primer pair (Mito & Endo, 1997) was used to amplify Hox-type homeobox fragments of the anterior and medial cognates in the Hox cluster; HoxME1 AAA AGG ATC CGA RCT NGA RAA RGA RTT (27mer) corresponding to amino acid sequence ELEKEF with Bam HI site, and HoxME2 AAA ACT GCA GYT TCA TNC GNC GRT TYT GRA ACC ADA TYT T corresponding to amino acid sequence IWFQNRRMK with Pst I site (40mer). Using this primer set, 143 bp of Hox-type homeobox fragments were obtained, including 76 bp of informative sequence spanning homeobox positions 60 -135 (amino acid positions 21 -45).…”

Section: Amplification Of Hox Gene Fragmentsmentioning

confidence: 99%

Evolution of Hox genes in molluscs: a comparison among seven morphologically diverse classes

Iijima

Akiba

Sarashina

et al. 2006

Journal of Molluscan Studies

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Hox genes have pivotal roles in animal body plan formation. We identified Hox genes and inferred the organizations of the Hox gene hypothetical ancestral cluster by a polymerase chain reaction (PCR) survey for the phylum Mollusca, so as to assess possible correlations with morphological diversity. All the extant classes, except for Monoplacophora, were examined, namely Solenogastres, Caudofoveata, Polyplacophora, Cephalopoda, Gastropoda, Scaphopoda, and Bivalvia. The result showed that the molluscs primarily possessed anterior, medial and posterior cognates of the Hox genes, ParaHox genes (Gsx, Xlox, and Cdx homologues), as well as non-Hox/ParaHox type homeobox genes (Gbx and Msx homologues). The last common ancestor of molluscs is inferred to have had twelve Hox genes: three anterior, seven medial and two posterior cognates. These results suggest that some of the anterior or posterior cognates have been lost in some lineages, most notably in Caudofoveata, while the medial cognates showed both losses and duplications. One of the medial cognates was found to be duplicated in all the classes examined except for Gastropoda, but not in other Metazoa, suggesting that this feature could be a synapomorphy of molluscs.

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“…Thus the relations between the developmental domains of expression of given echinoderm Hox genes and their respective genomic positions might illuminate the evolutionary transformations that led to the appearance of their unique morphological features. (iii) Though fragments of many individual Hox genes had been isolated from sea urchins (7)(8)(9)(10)(11) and starfish (12) by PCR and other methods, their specific classification (and hence the interpretation of their patterns of expression) in many cases required knowledge of their relative genomic positions. Furthermore, (iv) Strongylocentrotus purpuratus, the subject of this work, is a maximal indirect developer (13).…”

mentioning

confidence: 99%

Organization of an echinoderm Hox gene cluster

Martinez

Rast

Arenas-Mena

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

106

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The Strongylocentrotus purpuratus genome contains a single ten-gene Hox complex >0.5 megabase in length. This complex was isolated on overlapping bacterial artificial chromosome and P1 artificial chromosome genomic recombinants by using probes for individual genes and by genomic walking. Echinoderm Hox genes of Paralog Groups (PG) 1 and 2 are reported. The cluster includes genes representing all paralog groups of vertebrate Hox clusters, except that there is a single gene of the PG4-5 types and only three genes of the PG9-12 types. The echinoderm Hox gene cluster is essentially similar to those of the bilaterally organized chordates, despite the radically altered pentameral body plans of these animals.

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A PCR Survey of Hox Genes in the Sea Star,Asterina minor

Cited by 23 publications

References 29 publications

PCR Survey of Hox Genes in the Crinoid and Ophiuroid: Evidence for Anterior Conservation and Posterior Expansion in the Echinoderm Hox Gene Cluster

PCR Survey of Hox Genes in the Crinoid and Ophiuroid: Evidence for Anterior Conservation and Posterior Expansion in the Echinoderm Hox Gene Cluster

Evolution of Hox genes in molluscs: a comparison among seven morphologically diverse classes

Organization of an echinoderm Hox gene cluster

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