PLoS ONE volume 9, issue 6, Pe98282 2014 DOI: 10.1371/journal.pone.0098282 View full text
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Chuan Yu, Yaguang Zhang, Shaohua Yao, Yuquan Wei

Abstract: Genome editing techniques such as the zinc-finger nucleases (ZFNs), transcription activator-like effecter nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system Cas9 can induce efficient DNA double strand breaks (DSBs) at the target genomic sequence and result in indel mutations by the error-prone non-homologous end joining (NHEJ) DNA repair system. Several methods including sequence specific endonuclease assay, T7E1 assay and high resolution me…

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