A Pattern Recognition Based Fluorescence Quenching Assay for the Detection and Identification of Nitrated Explosive Analytes

Abstract

Herein we report a differential array of micelle-solubilized fluorophores for the detection and identification of small nitrated analytes, such as the explosives TNT, tetryl, RDX and HMX. The quenching ability of the analytes can be used to correlate their analyte identity, wherein the quenching patterns generated from the differential array are used in linear discriminant analysis (LDA). LDA results in a well-clustered two-dimensional plot, and a jack-knife analysis of the data suggests that this system can be used to identify unknown samples of analyte with 96 % accuracy and with a detection limit of 19 muM.

Amphiphilic metalloaggregates: Catalysis, transport, and sensing

“…14a) using pyrene solubilized in a micellar Tween 80 solution [73]. These aromatic compounds are known to quench the fluorescence of pyrene as well as other polyaromatic hydrocarbons, 15.…”

Exploitation of Guest Accessible Aliphatic Amine Functionality of a Metal–Organic Framework for Selective Detection of 2,4,6-Trinitrophenol (TNP) in Water

“…2,24 On the flipside, fluorescent probe based method harnessed from photoluminescence (PL)-based chemosensors possess unparalleled sensitivity, selectivity, low instrumentation cost, portability, short response time, and dual compatibility in solid and solution media. 5,[30][31][32] Several luminescent probes have been explored as fluorescence based explosive-sensors till date, 12,22,[33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] but their multi-step processing, toxicity and lack of control over molecular organization limits their wide use. 8,51 In fact, literature reports on fluorophore probe-based explosive sensing in water have been indeed scarce.…”

Fluorescent Nanofilms for Nitroaromatic Explosives Detection-Promises and Challenges

“…A crucial example is the detection of trace amount of 2,4,6-trinitrotoluene (TNT), which is a typical nitro-aromatic explosives [4][5][6][7][8]. Various TNT detection methods are currently available, such as, the fluorescence quenching method, micromechanical sensors based on micro cantilever, the electrochemical method, ion mobility s pectroscopy (IMS), the surface-enhanced Raman spectroscopy ( SERS) and semiconductor based gas sensors [7,[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. Among t he above-mentioned methods used for TNT detection, the fluorescence quenching method is considered to be the most effective tool for sensing nitro-aromatic explosives in recent years owing to its high sensitivity, convenience, easy visualization, and short response t ime for detection [24].…”