A Partial Denaturation Map of Herpes Simplex Virus Type 1 DNA: Evidence for Inversions of the Unique DNA Regions

Delius, H.; Clements, J. B.

doi:10.1099/0022-1317-33-1-125

Cited by 150 publications

(97 citation statements)

References 14 publications

(5 reference statements)

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“…This is found in regions thought to be polypeptide-coding, as well as in non-coding sequences. Examination of the partial denaturation maps of HSV-1 DNA produced by Delius & Clements (1976) shows that this region is one of the most G + C-rich in the HSV genome. The nature of the evolutionary forces producing this result is unknown.…”

Section: Discussionmentioning

confidence: 99%

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

Murchie¹,

Mcgeoch²

1982

Journal of General Virology

113

122

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SUMMARYThe nucleotide sequence of 4 kilobases of DNA from within the short region of the genome of herpes simplex virus type 1 has been determined. This portion of DNA contains the junctions of the terminal and internal inverted repeat sequence components with the unique sequence component and the 5' regions of the genes which encode the Vmw 12, Vmw 68 and Vmw 175 immediate-early polypeptides. The transcription and translation initiation sites of all three genes and the 5' and 3' boundaries of the Vmw 12 and Vmw 68 gene introns have been localized on the DNA sequence and shown to be flanked by sequences which resemble those found in similar positions in other eukaryotic genes. For the Vmw 12 and Vmw 68 genes the promoters, the 5'-non-coding regions of the mRNAs, and the introns lie within the terminal and internal inverted repeats respectively while the polypeptide-coding regions lie in the short unique component. The introns consist largely of tandemly reiterated copies of a 22-nucleotide sequence: the Vmw 12 gene intron contains seven of these and the Vmw 68 gene intron five. The Vmw 175 gene is located entirely within the short repeats, of which those regions sequenced here have the extremely high G + C content of 78 %, in marked contrast to the value of 66 % obtained for the adjacent region of the unique sequence component. Prediction of the complete amino acid sequence of the Vmw 12 polypeptide, accounting for a mol. wt. of 9830, and of the first 523 amino-terminal amino acids of the Vmw 175 polypeptide has been made from the DNA sequence. The polypeptide-coding region of the Vmw 175 gene has an average G + C content of 80% but nevertheless specifies a wide range of amino acid types because of the maximal assignment of G and C residues to codon third base positions.

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Section: Discussionmentioning

confidence: 99%

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

Murchie¹,

Mcgeoch²

1982

Journal of General Virology

113

122

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“…J. Davison & N. M. Wilkie, unpublished results). The L and S segments usually invert freely about the joint, resulting in equal amounts of both orientations of each segment in virion DNA (Hayward et al, 1975 b;Clements et aL, 1976: Delius & Clements, 1976.…”

Section: Introductionmentioning

confidence: 99%

One Functional Copy of the Long Terminal Repeat Gene Specifying the Immediate-early Polypeptide IE 110 Suffices for a Productive Infection of Human Foetal Lung Cells by Herpes Simplex Virus

Davison¹,

Marsden²,

Wilkie³

1981

Journal of General Virology

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SUMMARYThe HSV-1/HSV-2 intertypic recombinant Bxl (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (IR L and TR L) and expresses both the immediate-early polypeptide IE 110 of HSV-1 and its functionally equivalent polypeptide IE 118 of HSV-2. The genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. Subclone 14 lacked most of the IR r sequence, including the region from which part of the mRNA for IE 110 is transcribed, and expressed only the HSV-2 IE 118. Subclone 22 lacked almost all of TR r including the gene for IE 118 and induced only the HSV-1 IE 110. Since both subclones produced viable progeny in HFL cells it follows that expression of only one copy of the equivalent genes in TR L and IR L, here coding for the distinguishable polypeptides IE 110 or IE 118, is sufficient for successive complete cycles of virus replication.

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“…27 EndoG may be the nuclease responsible for the generation of double-stranded breaks at the HSV-1a sequence, which initiates the recombinational event that triggers the HSV-1a segment inversion. 28,29 Here, we demonstrate that yeast cells disrupted in EndoG show no polyploidization, which is in striking contrast to isogenic yeast controls in which up to 20% of the cells are polyploid. Consistently, depletion of mammalian EndoG selectively kills tetraploid human cells.…”

Section: Introductionmentioning

confidence: 63%

Depletion of Endonuclease G Selectively Kills Polyploid Cells

Büttner

Carmona-Gutierrez

Vitale³

et al. 2007

Cell Cycle

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Endonuclease G is a mitochondrio-nuclear located nuclease with dual-vital and lethal-functions. Besides its role in apoptosis execution, we have recently shown that depletion of endonuclease G leads to necrotic cell death in yeast. Here, we present further mechanistic elucidation of endonuclease G's vital functions. The deletion of the yeast Endonuclease G gene causes the complete elimination of tetraploid cells during exponential growth. Consistently, conditional knockdown of mammalian endonuclease G selectively kills tetraploid but not diploid clones of the human HCT116 colon carcinoma cell line. We conclude that endonuclease G is important for the viability of polyploid mammalian and yeast cells.

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A Partial Denaturation Map of Herpes Simplex Virus Type 1 DNA: Evidence for Inversions of the Unique DNA Regions

Cited by 150 publications

References 14 publications

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

DNA Sequence Anaylysis of an Immediate-Early Gene Region of the Herpes Simplex Virus Type 1 Genome (Map Coordinates 0.950 to 0.978)

One Functional Copy of the Long Terminal Repeat Gene Specifying the Immediate-early Polypeptide IE 110 Suffices for a Productive Infection of Human Foetal Lung Cells by Herpes Simplex Virus

Depletion of Endonuclease G Selectively Kills Polyploid Cells

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