A Parallel Synthesis Scheme for Generating Libraries of DNA Polymerase Substrates and Inhibitors

New phosphoramidite derivatives for the preparation of oligonucleotides containing a hydrazide group in any specified position of the oligonucleotide chain

“…The reaction requires no activating reagents; it occurs rapidly under mild conditions and gives products in high yields. [11][12][13][14][15] This method has a number of advan tages over the reaction of amines with aldehydes, which yields Schiff´s bases. 11 The latter are less stable than hydrazones, and additional reduction for the formation of more stable secondary amines is often required.…”

“…The existing methods are not versatile and suffer from some drawbacks. [11][12][13][14][15]20 Oligonucleotides containing the hy drazide group at the 5´ or 3´ end are commercially avaible from Metabion. 13 Their synthesis is based on phos phoramidite derivative 1 developed by the Nanogen Recognomics company.…”

New phosphoramidite derivatives for the preparation of oligonucleotides containing a hydrazide group in any specified position of the oligonucleotide chain

“…The reaction requires no activating reagents; it occurs rapidly under mild conditions and gives products in high yields. [11][12][13][14][15] This method has a number of advan tages over the reaction of amines with aldehydes, which yields Schiff´s bases. 11 The latter are less stable than hydrazones, and additional reduction for the formation of more stable secondary amines is often required.…”

“…The existing methods are not versatile and suffer from some drawbacks. [11][12][13][14][15]20 Oligonucleotides containing the hy drazide group at the 5´ or 3´ end are commercially avaible from Metabion. 13 Their synthesis is based on phos phoramidite derivative 1 developed by the Nanogen Recognomics company.…”

New phosphoramidite derivatives for the preparation of oligonucleotides containing a hydrazide group in any specified position of the oligonucleotide chain

“…The reaction requires no activating reagents; it occurs rapidly under mild conditions and gives products in high yields. [11][12][13][14][15] This method has a number of advan tages over the reaction of amines with aldehydes, which yields Schiff´s bases. 11 The latter are less stable than hydrazones, and additional reduction for the formation of more stable secondary amines is often required.…”

“…The existing methods are not versatile and suffer from some drawbacks. [11][12][13][14][15]20 Oligonucleotides containing the hy drazide group at the 5´ or 3´ end are commercially avaible from Metabion. 13 Their synthesis is based on phos phoramidite derivative 1 developed by the Nanogen Recognomics company.…”

New phosphoramidite derivatives for the preparation of oligonucleotides containing a hydrazide group in any specified position of the oligonucleotide chain

“…The reaction requires no activating reagents; it occurs rapidly under mild conditions and gives products in high yields. [11][12][13][14][15] This method has a number of advan tages over the reaction of amines with aldehydes, which yields Schiff´s bases. 11 The latter are less stable than hydrazones, and additional reduction for the formation of more stable secondary amines is often required.…”

“…The existing methods are not versatile and suffer from some drawbacks. [11][12][13][14][15]20 Oligonucleotides containing the hy drazide group at the 5´ or 3´ end are commercially avaible from Metabion. 13 Their synthesis is based on phos phoramidite derivative 1 developed by the Nanogen Recognomics company.…”