A obesidade é um determinante da resistência à insulina mais importante do que os níveis circulantes de citocinas pró‐inflamatórias em pacientes com artrite reumatoide

Castillo-Hernández, Jesús; Maldonado‐Cervantes, Martha Imelda; Reyes, Juan Pablo; Patiño-Marín, Nuria; Maldonado‐Cervantes, Enrique; Solorzano‐Rodriguez, Claudia; Mendoza, Esperanza de la Cruz; Alvarado‐Sánchez, Brenda

doi:10.1016/j.rbr.2016.10.005

Cited by 4 publications

(3 citation statements)

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“…corticoid use), play important roles in body weight . These individuals present increased or unchanged body weight, contrary to what is observed in cancer cachexia . Concerning the mentioned information, here we have not studied any pharmacological treatment in the CIA model and still the animals had not lost weight.…”

Section: Discussionmentioning

confidence: 86%

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Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Alabarse

Lora

Silva

et al. 2018

J cachexia sarcopenia muscle

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BackgroundRheumatoid arthritis is characterized by chronic polyarticular synovitis and presents systemic changes that impact quality of life, such as impaired muscle function, seen in up to 66% of the patients. This can progress to severely debilitating state known as rheumatoid cachexia—without loss of fat mass and body weight—for which there is little consensus in terms of diagnosis or treatment. This study aims to evaluate whether the collagen‐induced arthritis (CIA) animal model also develops clinical and functional features characteristic of rheumatoid cachexia.MethodsMale DBA1/J mice were randomly divided into 2 groups: healthy animals (CO, n = 11) and CIA animals (n = 13). The clinical score and edema size, animal weight and food intake, free exploratory locomotion, grip strength, and endurance exercise performance were tested 0, 18, 35, 45, 55, and 65 days after disease induction. After euthanasia, several organs, visceral and brown fat, and muscles were dissected and weighed. Muscles were used to assess myofiber diameter. Ankle joint was used to assess arthritis severity by histological score. Statistical analysis were performed using one‐way and two‐way analyses of variance followed by Tukey's and Bonferroni's test or t‐test of Pearson and statistical difference were assumed for a P value under 0.05.ResultsThe CIA had significantly higher arthritis scores and larger hind paw edema volumes than CO. The CIA had decreased endurance exercise performance total time (fatigue; 23, 22, 24, and 21% at 35, 45, 55, and 65 days, respectively), grip strength (27, 55, 63, 60, and 66% at 25, 35, 45, 55, and 65 days, respectively), free locomotion (43, 57, 59, and 66% at 35, 45, 55, and 65 days, respectively), and tibialis anterior and gastrocnemius muscle weight (25 and 24%, respectively) compared with CO. Sarcoplasmic ratios were also reduced in CIA (TA: 23 and GA: 22% less sarcoplasmic ratio), confirming the atrophy of skeletal muscle mass in these animals than in CO. Myofiber diameter was also reduced 45% in TA and 41% in GA in CIA when compared with the CO. Visceral and brown fat were lighter in CIA (54 and 39%, respectively) than CO group.ConclusionsThe CIA model is a valid experimental model for rheumatoid cachexia given that the clinical changes observed were similar to those described in patients with rheumatoid arthritis.

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Section: Discussionmentioning

confidence: 86%

“…Several studies have demonstrated that the increased TNFα and IL‐6 levels observed in RA patients, in conjunction with certain pharmacological treatments (e.g. corticoid use), play important roles in body weight . These individuals present increased or unchanged body weight, contrary to what is observed in cancer cachexia .…”

Section: Discussionmentioning

confidence: 99%

Collagen‐induced arthritis as an animal model of rheumatoid cachexia

Alabarse

Lora

Silva

et al. 2018

J cachexia sarcopenia muscle

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“…Isso mostra que, possivelmente, as alterações fisiopatológicas desta doença estejam presentes vários anos antes de sua expressão clínica (Gaydoso-Diz et al, 2013). O estresse oxidativo gerado pela dislipidemia (Sarto et al, 2018), assim como a liberação de várias citocinas pró-inflamatórias (Castillo-Hernandez et al, 2017) estão envolvidas na patogênese da resistência insulínica e muitas estratégicas anti-inflamatórias demonstraram melhorar a sensibilidade à insulina (Oliveira et al, 2016).…”

Section: Introductionunclassified

Efeito do extrato das folhas de amoreira (Morus nigra L.) no processo inflamatório cardiovascular e na resistência insulínica de camundongos dislipidêmicos

Pereira

Faria

Carvalho

et al. 2022

RSD

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Objetivo: Avaliar o efeito do extrato das folhas da amoreira na inflamação cardiovascular, na resistência insulínica e na hipertrofia ventricular esquerda de camundongos dislipidêmicos. Metodologia: Camundongos knockout para o gene do receptor LDL foram divididos em quatro grupos experimentais (n=20): grupo S (Standard), alimentados com dieta padrão; grupo SA (Standard /Amoreira), alimentados com dieta padrão e extrato das folhas da amoreira; grupo HL (Hyperlipidic), alimentados com dieta hiperlipídica e grupo HLA (Hyperlipidic/Amoreira), alimentados com dieta hiperlipídica e extrato das folhas da amoreira. Após 15 dias de experimento, foi coletado plasma e quantificado os níveis plasmáticos de colesterol total e suas frações, triglicerídeos, proteína C reativa, insulina, glicose e calculado o índice de Homa. Foram coletados tecidos cardíacos e da parede da aorta para quantificar a expressão do CD40L pelo método de western blotting. Lâminas histológicas com tecido cardíaco foram preparadas e coradas com hematoxilina e eosina, para avaliação histológica geral, e coradas com picrosírius red para avaliar a área de depósito de colágeno. Resultados: O extrato das folhas de amoreira elevou o HDL plasmático, preveniu moderadamente a hipercolesterolemia e não reduziu a hipertrigliceridemia nos camundongos do grupo HLA, preveniu a resistência à insulina e, consequentemente a hiperinsulinemia, reduziu o nível plasmático de PCR e preveniu o aumento da expressão do CD40L. Conclusão: O tratamento com o extrato das folhas de amoreira preveniu a inflamação cardiovascular e a resistência insulínica, reduzindo desta forma, o desenvolvimento da hipertrofia ventricular esquerda.

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