A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice by <i>in situ</i> bioluminescence imaging

Zhao, Ning; Liu, Jingmin; Liu, Shuang; Ji, Xiaofu; Lv, Huan; Hu, Yaozhong; Wang, Zhi-Hao; Lv, Shi‐Wen; Li, Chunyang; Wang, Shuo

doi:10.1039/d0ra01283a

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…Given the increasing interest in the potential of bacteria-mediated therapy, better analytical tools need to be developed to image the distribution of the bacteria. Bacterial luciferase reporters, , fluorescent proteins, and positron emission tomography have been used for the imaging of tumor-targeting bacteria and tumor detection, and in recent years, there has been considerable focus on using bioluminescence imaging , and fluorescence imaging methods − for bacteria in a variety of applications related to human health. Our study suggests that bioluminescence imaging is an optimal choice for the noninvasive, real-time tracing of bacteria and tumor detection in live animals.…”

Section: Discussionmentioning

confidence: 99%

Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging

et al. 2021

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The anticancer therapy strategy mediated by tumor-targeting bacteria needs better visualization tools for imaging and monitoring bacteria in vivo. The probiotic strain Escherichia coli Nissle 1917 (EcN), one of the tumor-targeting bacteria, leads to the potential application for cancer therapy. Here, we report the development and application of a live, EcN-based imageable vehicle for noninvasive in vivo bioluminescence imaging in live mice. Firefly luciferase (Fluc) and luciferin-regenerating enzyme (LRE), an enzyme that contributes to stable bioluminescence, were functionally coexpressed in EcN. The recombinant EcN strain expressing the genomically integrated Fluc-LRE cassette was demonstrated to be a valuable tool for generating robust, continuous, and red-shifted bioluminescence for bacterial tracking in vitro and in vivo, thus providing an optical tumor-targeting system for the in vivo study of bacteria-assisted cancer therapy. Additionally, in vivo imaging of the recombinant EcN strain in the mouse intestinal tract indicated the potential of this strain to be used as a tool in the study of gut.

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Section: Discussionmentioning

confidence: 99%

Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging

et al. 2021

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“…Bioluminescence labeled with luciferases is an effective tool for real-time monitoring and non-damaged detection of target bacteria, which aims to collect more accurate and specific information for further research. , In our previous work, a novel universal nanoluciferase-involved reporter (NLuc) system was established. The NLuc system exhibited various incomparable advantages, especially in the application of tracking the target bacteria’s precise localization …”

Section: Introductionmentioning

confidence: 99%

“…The NLuc system exhibited various incomparable advantages, especially in the application of tracking the target bacteria's precise localization. 19 T h i s c o n t e n t i s Based on the investigation above, we hypothesized that ironenriched probiotics may achieve both being iron supplements and alleviating the symptom of UC. In this study, a novel mediation strategy of dextran sodium sulfate (DSS)-induced colitis in mice based on an iron-enriched probiotic and bioluminescence tracing was established.…”

Section: ■ Introductionmentioning

confidence: 99%

A Novel Mediation Strategy of DSS-Induced Colitis in Mice Based on an Iron-Enriched Probiotic and In Vivo Bioluminescence Tracing

Zhao

Liu

Yang

et al. 2020

J. Agric. Food Chem.

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Iron deficiency (ID) caused by blood loss and/or reduced iron absorption is a serious problem influencing health in inflammatory bowel disease (IBD). However, traditional iron supplements may fail to meet no side effect demands for ID of IBD; thus, a new iron supplementation is highly desired to be developed. Herein, for the first time, probiotic Lactobacillus alimentarius NKU556 with an iron-enriching ability was screened from Chinese traditional fermented food then employed to intervene DSSinduced colitis with bioluminescence tracing in mice. As expected, oral administration with NKU556-Fe can remarkably enhance the expression of tight junction proteins and effectively reduce the pro-inflammatory cytokines as well as the oxidative stress on DSSinduced colitis in mice. Meanwhile, in comparison with the FeSO 4 group, the intake of NKU556-Fe could suppress the expression of hepcidin derived from the liver and reduce the degradation of FPN1, thereby leading to the increase in the iron absorption of colitis in mice. According to the bioluminescence result, it was believed that the beneficial effects of oral administration with NKU556/ NKU556-Fe on DSS-induced colitis in mice were hardly related to its metabolites but associated with its own function. These results concluded that the oral administration of NKU556-Fe could relieve colitis inflammation and increase iron absorption. In summary, current work not only proposed a novel mediation strategy for IBD but also offered some inspirations for future treatment of extraintestinal complications.

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DNA-mediated Au@Ag@silica nanopopcorn fluorescent probe for in vivo near-infrared imaging of probiotic Lactobacillus Plantarum

Gao

Zhou

et al. 2022

Biosensors and Bioelectronics

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A novel universal nano-luciferase-involved reporter system for long-term probing food-borne probiotics and pathogenic bacteria in mice by in situ bioluminescence imaging

Abstract: Schematic illustration of the preparation of bioluminescent bacteria and the experimental design of tracing of the foodborne bacteria in vivo.

Cited by 4 publications

References 42 publications

Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging

Bacteria-Based Live Vehicle for In Vivo Bioluminescence Imaging

A Novel Mediation Strategy of DSS-Induced Colitis in Mice Based on an Iron-Enriched Probiotic and In Vivo Bioluminescence Tracing

DNA-mediated Au@Ag@silica nanopopcorn fluorescent probe for in vivo near-infrared imaging of probiotic Lactobacillus Plantarum

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