A novel trap design for non-lethal monitoring of dung beetles using eDNA metabarcoding

Leandro, Camila; Déjean, Tony; Valentini, Alice; Jean, Pauline; Jay‐Robert, Pierre

doi:10.1007/s10841-021-00329-4

Cited by 3 publications

(7 citation statements)

References 79 publications

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“…In comparison, the method proposed by Leandro et al (2021) showed congruence with the results of improved traditional trapping but was restricted to Scarabaeoidea dung beetle species.…”

Section: Diversity Of Materials and Methods In Edna Studiesmentioning

confidence: 82%

“…Despite some previous potential drawbacks on the use of soil for eDNA (Harrison et al, 2019;Prosser & Hedgpeth, 2018;Taberlet et al, 2018;Valentin et al, 2021), it was the dominant used matrix with 19 mentions, followed by faecal material with 13 mentions (i.e., dung, guano), plant material with eight mentions (flowers, litter or directly the plants) and arthropod material (honey, spider webs, honeydew, etc.). A methodological path that seems promising is the use of water or other liquid solutions as physiological serum with other media, such as vegetation (washed fruits [Valentin et al, 2018[Valentin et al, , 2020 or leaves with rain [Macher et al, 2023]) or in non-destructive DNA collection devices (Leandro et al, 2021: their 'Non Destructive Collector' (NDC) device imitates a small pond). Although water is an environmental matrix that comes with its own particular challenges for eDNA studies (Hinlo et al, 2017;Majaneva et al, 2018), it is a collecting substance that can help bypass the homogenization problem of terrestrial matrices such as soil, litter or vegetation, where vertical stratification or the use by targeted taxa might render the localization of DNA more random (Barnes & Turner, 2016).…”

Section: Diversity Of Materials and Methods In Edna Studiesmentioning

confidence: 99%

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eDNA for monitoring and conserving terrestrial arthropods: Insights from a systematic map and barcode repositories assessments

Leandro,

Jay‐Robert,

Pétillon

2024

Insect Conserv Diversity

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In the past decade, environmental DNA (eDNA) assays have become an essential tool to investigate species presence with samples from the environment instead of collected specimens. eDNA sampling techniques have proved their worth in freshwater and marine studies; now, some trends emerge for their use in terrestrial habitats and particularly to study arthropods. After a systematic review of the literature, we illustrate and analyse the diversity of such studies and discuss their benefits and drawbacks. We identified the most relevant research themes and focused on (i) the taxa and environmental sample types targeted and (ii) the details of the survey scheme. In parallel, we also assessed the available number of sequences from cytochrome c oxidase subunit I (COI), 16S and 18S barcode regions for four major taxa (spiders, centipedes, springtails and insects) in relation to their diversity. We found strong taxonomic and geographic biases regarding coverage per barcode. eDNA research on terrestrial arthropods mainly focuses on insect species that affect humanity in a positive or negative way, and the availability of sequences is much higher for species from temperate‐developed countries than from tropical ones. Moreover, although a high variety of environmental samples are being used, most studies do not assess the barcode completeness of the target taxa nor compare the efficacy of eDNA monitoring technique to other well established and known traditional techniques. Careful workflow designs and comparisons are needed before giving any management or conservation advice as eDNA monitoring does not come without error. Strengths and weaknesses of eDNA assays for conservation are discussed.

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“…In comparison, the method proposed by Leandro et al (2021) showed congruence with the results of improved traditional trapping but was restricted to Scarabaeoidea dung beetle species.…”

Section: Diversity Of Materials and Methods In Edna Studiesmentioning

confidence: 82%

Section: Diversity Of Materials and Methods In Edna Studiesmentioning

confidence: 99%

eDNA for monitoring and conserving terrestrial arthropods: Insights from a systematic map and barcode repositories assessments

Leandro,

Jay‐Robert,

Pétillon

2024

Insect Conserv Diversity

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“…Molecular methods identify the presence of species from their molecular traces (typically DNA or RNA), which can be extracted from collected insects [23], from a trapping medium (e.g. [24]), or from the wider environment (eDNA; [25][26][27]). The widespread use of such methods was kickstarted by the seminal contributions of Hebert et al [23], who proposed that short pieces of standardized marker genes could serve as reliable keys to species identification.…”

Section: (A) Molecular Methodsmentioning

confidence: 99%

Towards a toolkit for global insect biodiversity monitoring

van Klink,

Sheard,

Høye

et al. 2024

Phil. Trans. R. Soc. B

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Insects are the most diverse group of animals on Earth, yet our knowledge of their diversity, ecology and population trends remains abysmally poor. Four major technological approaches are coming to fruition for use in insect monitoring and ecological research—molecular methods, computer vision, autonomous acoustic monitoring and radar-based remote sensing—each of which has seen major advances over the past years. Together, they have the potential to revolutionize insect ecology, and to make all-taxa, fine-grained insect monitoring feasible across the globe. So far, advances within and among technologies have largely taken place in isolation, and parallel efforts among projects have led to redundancy and a methodological sprawl; yet, given the commonalities in their goals and approaches, increased collaboration among projects and integration across technologies could provide unprecedented improvements in taxonomic and spatio-temporal resolution and coverage. This theme issue showcases recent developments and state-of-the-art applications of these technologies, and outlines the way forward regarding data processing, cost-effectiveness, meaningful trend analysis, technological integration and open data requirements. Together, these papers set the stage for the future of automated insect monitoring. This article is part of the theme issue ‘Towards a toolkit for global insect biodiversity monitoring’.

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“…However, eDNA adsorbed to soil particles may remain preserved from degradation and not represent the current occupancy of a species (Cai et al, 2006; Demanèche et al, 2001; Kirtane et al, 2020). Recently, species from Diptera and Coleoptera orders were identified using eDNA metabarcoding via non‐invasive baited traps (Camila et al, 2021). However, few studies have focused on eDNA analysis directly from plant surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, species from Diptera and Coleoptera orders were identified using eDNA metabarcoding via non-invasive baited traps (Camila et al, 2021). However, few studies have focused on eDNA analysis directly from plant surfaces.…”

Section: Introductionmentioning

confidence: 99%

Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

et al. 2022

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Environmental DNA (eDNA) analysis can be a powerful tool for the early detection of invasive organisms. However, research on terrestrial eDNA detection from foliage surfaces has been limited. In this study, we developed methods to capture and detect eDNA using qPCR from an invasive forest pest, hemlock woolly adelgid (Adelges tsugae), and three of its biological control predators Leucotaraxis piniperda, Leucotaraxis argenticollis, and Laricobius nigrinus. We designed four highly efficient qPCR assays with a low limit of detection (1-10 copies/reaction). The assay targeting A. tsugae was species-specific. The assays targeting Le. piniperda, and Le. argenticollis were biotype-specific in addition to being species-specific demonstrating applications of eDNA analysis beyond species-level detection. The La. nigrinus assay also detected DNA from closely related and hybridizing Laricobius rubidus. The eDNA methods were evaluated against traditional detection methods. We collected foliage samples from three strata (bottom, middle, and top) of eastern hemlock trees to detect the presence of A. tsugae. The detection of the biological control predators was evaluated using western hemlock foliage samples collected from the predators' native range in western Washington. The eDNA methods had significantly higher positive detection rates (2.8-4.5 times) than conventional methods of all target species. The strata of sampling were not significant in determining the presence of A. tsugae infestation.The eDNA concentration positively correlated with the observed density for all species. This study demonstrates the efficacy of eDNA analysis as a more sensitive tool for early detection of A. tsugae and to track the establishment of its biological control predators.

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A novel trap design for non-lethal monitoring of dung beetles using eDNA metabarcoding

Cited by 3 publications

References 79 publications

eDNA for monitoring and conserving terrestrial arthropods: Insights from a systematic map and barcode repositories assessments

eDNA for monitoring and conserving terrestrial arthropods: Insights from a systematic map and barcode repositories assessments

Towards a toolkit for global insect biodiversity monitoring

Sensitive environmental DNA (eDNA) methods to detect hemlock woolly adelgid and its biological control predators Leucotaraxis silver flies and a Laricobius beetle

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