A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

Abstract

A salt- and drought-responsive novel gene SbSDR1 is predominantly localised to the nucleus, up-regulated under abiotic stresses and is involved in the regulation of metabolic processes. SbSDR1 showed DNA-binding activity to genomic DNA, microarray analysis revealed the upregulation of host stress-responsive genes and the results suggest that SbSDR1 acts as a transcription factor. Overexpression of SbSDR1 did not affect the growth and yield of transgenic plants in non-stress conditions. Moreover, the overexpression of SbSDR1 stimulates the growth of plants and enhances their physiological status by modulating the physiology and inhibiting the accumulation of reactive oxygen species under salt and osmotic stress. Transgenic plants that overexpressed SbSDR1 had a higher relative water content, membrane integrity and concentration of proline and total soluble sugars, whereas they showed less electrolyte leakage and lipid peroxidation than wild type plants under stress conditions. In field conditions, SbSDR1 plants recovered from stress-induced injuries and could complete their life cycle. This study suggests that SbSDR1 functions as a molecular switch and contributes to salt and osmotic tolerance at different growth stages. Overall, SbSDR1 is a potential candidate to be used for engineering salt and drought tolerance in crops without adverse effects on growth and yield.

Halotolerant PGPR Stenotrophomonas maltophilia BJ01 Induces Salt Tolerance by Modulating Physiology and Biochemical Activities of Arachis hypogaea

“…In stress condition plants overproduce reactive oxygen species (ROS) which act as a signaling molecule for downstream regulation of defense mechanism; this situation called oxidative stress ( Demidchik, 2015 ). Among the ROS, hydrogen peroxide (H 2 O 2 ) is considered as the most stable molecule ( Foyer and Noctor, 2009 ) and generate high concentration under salt stress ( Singh et al, 2016 ). Lower H 2 O 2 concentration in plants with bacterial inoculation in salt stress shows the beneficial effect of S. maltophilia BJ01 on peanut under stress conditions which reduces the oxidative stress on the plant system.…”

“…The hydrogen peroxide and the superoxide radicals in stressed and unstressed plant samples were determined in vivo using a histochemical stain of 3,3- diaminobenzidine (DAB) and nitro-blue tetrazolium (NBT) respectively ( Singh et al, 2016 ). Solutions of DAB and NBT were prepared in 10 mM phosphate buffer (pH 7.8).…”

Metabolic profiling and scavenging activities of developing circumscissile fruit of psyllium (Plantago ovata Forssk.) reveal variation in primary and secondary metabolites

“…Total lipid composition was statistically analysed by principal component analysis (PCA) and a heat map was generated. Lipid data set was used to generate Pearson's correlation matrix, and PCA along with respective heat map was inferred [55,61] using different software including Sigma Plot (ver. 12), SYSTAT (ver.…”

Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses

“…Tobacco has been widely applied in plant biological research. For example, nicotine synthesis regulation19, resistance to biotic and abiotic stress2021, influence of tocopherol on leaf senescence9, molecular phenotype of lignin-modified tobacco22, etc. In this study, the early senescence of tobacco leaves was investigated under natural developmental conditions by using integrated multi-platform metabolomics based on GC-MS, CE-MS and LC-MS. Broad metabolome coverage was achieved, including pigments, lipids, sugars, sterols, amino acids, polyamines and polyphenols.…”